Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	99,367	C→T	G397D (GGC→GAC)	SAUSA300_RS00475 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	99,367	0	C	T	100.0%	14.4 / NA	7	G397D (GGC→GAC)	SAUSA300_RS00475	MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/2);  total (5/2)

TGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGAT  >  NC_007793/99270‑99411                                                                                                  |                                             gtggccgtgGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGTCt                                            >  1:2460013/7‑100 (MQ=255)     ctgtgGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGTCTGttt                                        >  1:5941262/2‑100 (MQ=255)      tgtgGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGTCTGtttt                                       >  1:3208560/1‑100 (MQ=255)       gtgGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGTCTGTTTTg                                      >  1:831977/1‑100 (MQ=255)         gggTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGTCTGTTTTGtt                                    >  1:2917941/1‑100 (MQ=255)             gCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGTCTGTTTTGTTAACa                                <  1:3353061/100‑1 (MQ=255)                                           tCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGTCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGAt  <  1:4031670/100‑1 (MQ=255)                                                                                                  |                                             TGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGAT  >  NC_007793/99270‑99411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑