Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,058,202	T→C	intergenic (‑621/+184)	SAUSA300_RS05185 ← / ← SAUSA300_RS05190	hypothetical protein/bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase/5,10‑methylene‑tetrahydrofolate cyclohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,058,202	0	T	C	100.0%	225.9 / NA	81	intergenic (‑621/+184)	SAUSA300_RS05185/SAUSA300_RS05190	hypothetical protein/bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase/5,10‑methylene‑tetrahydrofolate cyclohydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/80);  total (1/80)

CTACTTTGAAGGGAGCATTTGATCGATTAATGTCCCAGATTTTTCTTGTTATTCATTGCAATTGCAAAATAATATTTAATGTGGGTTAAAAGACTTTATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACTAGAATTGAAAA  >  NC_007793/1058106‑1058297                                                                                                 |                                                                                                cTACTTTGAAGGGAGCATTTGATCGATTAATGTCCCAGATTTTTCTTGTTATTCATTGCAATTGCAAAATAATATTTAATGTGGGTTAAAAGACTtcatc                                                                                              <  1:3145144/100‑2 (MQ=255)                   ttGATCGATTAATGTCCCAGATTTTTCTTGTTATTCATTGCAATTGCAAAATAATATTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAggg                                                                            <  1:5682091/100‑1 (MQ=255)                     tcttccgatctGTCCCAGATTTTTCTTGTTATTCATTGCAATTGCAAAATAATATTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAg                                                                          <  1:1680897/90‑1 (MQ=255)                      cttccgatctGTCCCAGATTTTTCTTGTTATTCATTGCAATTGCAAAATAATATTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAgg                                                                         <  1:2683163/91‑2 (MQ=255)                       ttccgatctGTCCCGGATTTTTCTTGTTATTCATTGCAATTGCAAAATAATATTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTg                                                                        <  1:3806814/92‑1 (MQ=255)                        tccgatctGTCCCGGATTTTTCTTGTTATTCATTGCAATTGCAAAATAATATTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTgg                                                                       <  1:6551292/93‑1 (MQ=255)                        tccgatctGTCCCAGATTTTTCTTGTTATTCATTGCAATTGCAAAATAATATTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTgg                                                                       <  1:1355302/93‑1 (MQ=255)                        tccgatctGTCCCAGATTTTTCTTGTTATTCATTGCAATTGCAAAATAATATTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTgc                                                                       <  1:74861/93‑2 (MQ=255)                         ccgatctGTCCCAGATTTTTCTTGTTATTCATTGCAATTGCAAAATAATATTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTggg                                                                      <  1:6082445/94‑1 (MQ=255)                              aagTCCCAGATTTTTCTTGTTATTCATTGCAATTGCAAAATAATATTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGa                                                                 >  1:756279/3‑100 (MQ=255)                                gTCCCAGATTTTTCTTGTTATTCATTGCAATTGCAAAATAATATTTAATGTGGGTTAAAAGACTTCATTAAAGCTCAGTTATTAGGGAGTGGGACAGacc                                                               <  1:1791080/100‑3 (MQ=255)                                              ttGTTATTCATTGCAATTGCAAAATAATATTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTag                                                 <  1:2463045/100‑2 (MQ=255)                                                                         tatTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCTCCCCGGCa                      <  1:4454213/100‑1 (MQ=255)                                                                         tatTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCCCCCCGGCa                      <  1:846656/100‑1 (MQ=255)                                                                         tatTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCa                      <  1:3036037/100‑1 (MQ=255)                                                                         tatTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCa                      <  1:4735356/100‑1 (MQ=255)                                                                         tatTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCa                      <  1:269472/100‑1 (MQ=255)                                                                         tatTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCa                      <  1:1406345/100‑1 (MQ=255)                                                                         tatTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCa                      <  1:5625331/100‑1 (MQ=255)                                                                         tatTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCa                      <  1:3387359/100‑1 (MQ=255)                                                                         tatTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCa                      <  1:3787926/100‑1 (MQ=255)                                                                         tatTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCa                      <  1:4212215/100‑1 (MQ=255)                                                                         tatTTAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCa                      <  1:195488/100‑1 (MQ=255)                                                                           tttAATGTGGGTTAAAAGATTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAg                    <  1:2788698/100‑1 (MQ=255)                                                                           tttAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAg                    <  1:4650463/100‑1 (MQ=255)                                                                           cgatctgtgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAg                    <  1:6018348/95‑1 (MQ=255)                                                                            ttAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCaatg                   <  1:4554886/100‑3 (MQ=255)                                                                            ttAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAgg                   <  1:5333930/100‑1 (MQ=255)                                                                             tAATGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAgag                  <  1:5349103/100‑3 (MQ=255)                                                                              ctcgtgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGtc                 <  1:1626904/97‑2 (MQ=255)                                                                              aaTGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGtt                 <  1:5404552/100‑1 (MQ=255)                                                                              aaTGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGtt                 <  1:963115/100‑1 (MQ=255)                                                                              aaTGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGtt                 <  1:3729909/100‑1 (MQ=255)                                                                               aTGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTg                <  1:6154746/100‑1 (MQ=255)                                                                               aTGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTg                <  1:744426/100‑1 (MQ=255)                                                                               aTGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTg                <  1:1662433/100‑1 (MQ=255)                                                                               aTGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTg                <  1:6541436/100‑1 (MQ=255)                                                                               aTGTGGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTg                <  1:303345/100‑1 (MQ=255)                                                                                tgtgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTgg               <  1:3777572/100‑2 (MQ=255)                                                                                tgtgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGCGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTgg               <  1:4619560/100‑2 (MQ=255)                                                                                  tgGGTTAAAAGGCTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTGTTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:3270786/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTGAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:943114/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCTCCCCGGCAAGGTTGACt             <  1:6500336/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACTCCGGCAAGGTTGACt             <  1:3215715/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGAcc             <  1:3528600/100‑2 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGAcc             <  1:4036488/100‑2 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGAca             <  1:3012506/100‑2 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:5092528/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:5107937/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:5212344/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:575771/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:5715789/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:6350754/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:3067472/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:176223/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:2267795/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:829859/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:2342845/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:237238/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:4171928/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:1693964/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:3422461/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:37705/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:1101743/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:3872052/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTGCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:3776507/100‑1 (MQ=255)                                                                                  tgGGTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAATGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCCCCCCGGCAAGGTTGACt             <  1:3930610/100‑1 (MQ=255)                                                                                  tgGGTCAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACt             <  1:1766909/100‑1 (MQ=255)                                                                                   gggTTAAAAGACTTCATTAACACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACTa            <  1:2011116/100‑1 (MQ=255)                                                                                    ggTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACTaa           <  1:2780062/100‑2 (MQ=255)                                                                                    ggTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACTAg           <  1:3187311/100‑1 (MQ=255)                                                                                    gctcttccgACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACTAg           <  1:384161/92‑1 (MQ=18)                                                                                     gTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCTTCGTCCCACCCCGGCAAGGTTGACTAgt          <  1:6138501/100‑2 (MQ=255)                                                                                     gTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACTAgg          <  1:2339303/100‑2 (MQ=255)                                                                                     gTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACTAgg          <  1:208480/100‑2 (MQ=255)                                                                                     gTTAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACTAGa          <  1:4569498/100‑1 (MQ=255)                                                                                       tAAAAGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACTAGAAt        <  1:2015923/100‑1 (MQ=255)                                                                                         aaaGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACTAGAAttt      <  1:1134761/100‑2 (MQ=255)                                                                                         aaaGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACTAGAATTg      <  1:531659/100‑1 (MQ=255)                                                                                         aaaGACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACTAGAATTg      <  1:4279012/100‑1 (MQ=255)                                                                                            gACTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACTAGAATTGaaa   <  1:5320626/100‑1 (MQ=255)                                                                                             aCTTCATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCATCCCGGCAAGGTTGACTAGAATTGaaaa  <  1:498170/100‑1 (MQ=255)                                                                                                 |                                                                                                CTACTTTGAAGGGAGCATTTGATCGATTAATGTCCCAGATTTTTCTTGTTATTCATTGCAATTGCAAAATAATATTTAATGTGGGTTAAAAGACTTTATTAAAACTCAGTTATTAGGGAGTGGGACAGAAATGATATTTTCGTAAAATTTATTTCGTCGTCCCACCCCGGCAAGGTTGACTAGAATTGAAAA  >  NC_007793/1058106‑1058297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑