Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,046,861	A→C	L377V (TTA→GTA)	SAUSA300_RS05135 ←	bifunctional autolysin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,046,861	0	A	C	100.0%	156.3 / NA	57	L377V (TTA→GTA)	SAUSA300_RS05135	bifunctional autolysin
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (1/56);  total (1/56)

ATTAAATATTTTTCATTAATTAAGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTAAATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTCAGCACTGTCTGGTTTTAAACCATAATATTGTAATTGTGTAGC  >  NC_007793/1046764‑1046954                                                                                                  |                                                                                              attaAATATTTTTCATTAATTAAGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACat                                                                                             <  1:1081690/100‑1 (MQ=255) attaAATATTTTTCATTAATTAAGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACat                                                                                             <  1:5951252/100‑1 (MQ=255) attaAATATTTTTCATTAATTAAGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACat                                                                                             <  1:1495156/100‑1 (MQ=255) attaAATATTTTTCATTAATTAAGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACat                                                                                             <  1:337739/100‑1 (MQ=255)  ttaAATATTTTTCATTAATTAAGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCGCCTACata                                                                                            <  1:5144624/100‑1 (MQ=255)  ttaAATATTTTTCATTAATTAAGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACatt                                                                                            <  1:2274781/100‑2 (MQ=255)           tttCATTAATTAAGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATttacttac                                                                                   <  1:6216970/100‑1 (MQ=255)           tttCATTAATTAAGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATttacttac                                                                                   <  1:3968454/100‑1 (MQ=255)           tttCATTAATTAAGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATttacttaa                                                                                   <  1:4277397/100‑2 (MQ=255)           tttCATTAATTAAGCCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATttacttac                                                                                   <  1:4790784/100‑1 (MQ=255)                ttaattaaGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCGCCTACATATTTACTTACAGCGt                                                                              <  1:5399073/100‑1 (MQ=255)                ttaattaaGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCgg                                                                              <  1:3293566/100‑2 (MQ=255)                ttaattaaGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGt                                                                              <  1:4519276/100‑1 (MQ=255)                ttaattaaGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGt                                                                              <  1:3861558/100‑1 (MQ=255)                   attaaGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGt                                                                           <  1:5176704/100‑1 (MQ=255)                    ttaaGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTg                                                                          <  1:979405/100‑1 (MQ=255)                    ttaaGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTg                                                                          <  1:6491728/100‑1 (MQ=255)                    ttaaGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTg                                                                          <  1:5493566/100‑1 (MQ=255)                    ttaaGTCATATAATTGATCATAACTATAATTACGACTTCTTAAATATCCATGCTGATCGGCATGGTCAGTACCACCTACATATTTACTTCCAGCGTAGTg                                                                          <  1:6192268/100‑1 (MQ=255)                     taaGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTgg                                                                         <  1:5070149/100‑2 (MQ=255)                         tCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTcc                                                                     <  1:1109024/100‑1 (MQ=255)                          cATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCa                                                                    <  1:5084326/100‑1 (MQ=255)                           atatAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCAt                                                                   <  1:4268835/100‑1 (MQ=255)                            tataATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCAtt                                                                  <  1:2016529/100‑2 (MQ=255)                             ataATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATag                                                                 <  1:1566141/100‑2 (MQ=255)                             ataATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATAc                                                                 <  1:4731074/100‑1 (MQ=255)                                aTTGATCATAACTAGAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACTACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGt                                                              <  1:5002972/100‑1 (MQ=255)                                 ttGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACGACCTACATATTTACTTACAGCGTAGTGAGTCCATACAGtg                                                             <  1:2864320/100‑2 (MQ=255)                                 ttGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCGCCTACATATTTACTTACAGCGTAGTGAGTCCATACTGtc                                                             <  1:595792/100‑2 (MQ=255)                                 ttGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGtg                                                             <  1:806528/100‑2 (MQ=255)                                  tGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTAc                                                            <  1:1332790/100‑1 (MQ=255)                                   gATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTAcc                                                           <  1:658179/100‑1 (MQ=255)                                    aTCATAACTATAATTATGACTTCTTAAATATCCATGTGGAGCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTAccc                                                          <  1:3506945/100‑2 (MQ=255)                                     tCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGGCCATACTGTACCag                                                         <  1:1463169/100‑2 (MQ=255)                                              taATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTGCAGCGTAGTGAGTCCATACTGTGCCATTTCcatcat                                                <  1:5268705/100‑1 (MQ=255)                                              taATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCcatcaa                                                <  1:5979867/100‑2 (MQ=255)                                               aaTTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCcatcatc                                               <  1:4894289/100‑2 (MQ=255)                                                aTTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATAc                                              <  1:5010292/100‑1 (MQ=255)                                                 ttATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATagc                                             <  1:3573584/100‑3 (MQ=255)                                                  tATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTc                                            <  1:1563269/100‑1 (MQ=255)                                                  tATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTc                                            <  1:849864/100‑1 (MQ=255)                                                  tATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTc                                            <  1:255352/100‑1 (MQ=255)                                                   aTGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCcatcagatcgg                                           >  1:529232/1‑94 (MQ=255)                                                     gACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTCAGc                                         <  1:3918617/100‑1 (MQ=255)                                                      aCTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTCAGCa                                        <  1:5882905/100‑1 (MQ=255)                                                         tcttAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTCAGCACTg                                     <  1:3450892/100‑1 (MQ=255)                                                          cttAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTCAGCAcact                                    <  1:1673092/100‑4 (MQ=255)                                                          cttAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTCAGCACTGt                                    <  1:1708281/100‑1 (MQ=255)                                                           ttAAATATCCATGTGGATCGGCATGGTCAGTACCACCTACGTATTTACTTACAGCGTAGTGAGTCCATACGGTACCATTTCCATCATACTCAGCACTggg                                   <  1:4773793/100‑3 (MQ=255)                                                            tAAATATCCATGTGGATCGGCATGGTCAGTACCTCCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTCAGCACTGTca                                  <  1:18587/100‑2 (MQ=255)                                                            tAAATAACCATGTGGATCGGCATGGTCAGTTCCGCCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTCAGCACTGTCt                                  <  1:1982085/100‑1 (MQ=255)                                                              aaTATCCATGTGGATCGGCATGGTCAGTACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTCAGCACTGTCTgg                                <  1:5787651/100‑1 (MQ=255)                                                                                 aTGGTCAGTACCACCTGCATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTCAGCACTGTCTGGTTTTAAACCATAATATTGt             <  1:2332827/100‑1 (MQ=255)                                                                                         tACCACCTACATATTTACTTACAGCGTAGTGAGTCCATACTGTGCCATTTCCATCATACTCAGCACTGTCTGGTTTTAAACCATAATATTGTAATTgtgt     <  1:4658491/100‑1 (MQ=255)                                                                                         tACCACCTACATATTAGCTTCCAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTCAGCACTGTCTGGTTTTAAACCATAATATTGTAATTgtgt     <  1:2458516/100‑1 (MQ=255)                                                                                           ccaccTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTCAGCACTGTCTGGTTTTAAACCATAATATTGTAATTGTGTAg   <  1:3953977/100‑1 (MQ=255)                                                                                            caccTACATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTCAGCACTGTCTGGTTTTAAACCATAATATTGTAATTGTGTAGc  <  1:1026106/100‑1 (MQ=255)                                                                                                  |                                                                                              ATTAAATATTTTTCATTAATTAAGTCATATAATTGATCATAACTATAATTATGACTTCTTAAATATCCATGTGGATCGGCATGGTCAGTACCACCTAAATATTTACTTACAGCGTAGTGAGTCCATACTGTACCATTTCCATCATACTCAGCACTGTCTGGTTTTAAACCATAATATTGTAATTGTGTAGC  >  NC_007793/1046764‑1046954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑