Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,045,185	G→A	T935T (ACC→ACT)	SAUSA300_RS05135 ←	bifunctional autolysin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,045,185	0	G	A	100.0%	96.3 / NA	34	T935T (ACC→ACT)	SAUSA300_RS05135	bifunctional autolysin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/31);  total (3/31)

TTTGGTGTTACATAGTAATAACCATTACCATTTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCGGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTACAGATACTTGTTTCGTT  >  NC_007793/1045096‑1045281                                                                                          |                                                                                                 tttGGTGTTACATAGTAATAACCATTACCATTTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAg                                                                                        <  1:5611644/100‑1 (MQ=255)  ttGGTGTTACATAGTAATAACCATTACCATTTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGt                                                                                       <  1:6236288/100‑1 (MQ=255)    ggTGTTACATAGTAATAACCATTACCATTTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACGGCAGTTGGTGCAGTTa                                                                                     <  1:3717251/100‑1 (MQ=255)                      ccattaccattTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTAccc                                                                   <  1:5928587/100‑1 (MQ=255)                       cattaccattTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCa                                                                  <  1:2331411/100‑1 (MQ=255)                                 tttAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCgt                                                        <  1:5355825/100‑2 (MQ=255)                                    aaTTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGtta                                                     <  1:2288415/100‑1 (MQ=255)                                                 ctcttccgaTCTTTGGCGGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTGCCCAACCAGTGCGGTTATTAATAGTACCGt                                        <  1:5262842/92‑1 (MQ=255)                                                 ctcttccgaTCTCTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGt                                        <  1:843083/92‑1 (MQ=255)                                                  tcttccgaTCTTTGGCGGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGtg                                       <  1:5168012/93‑2 (MQ=255)                                                   cttccgaTCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGtat                                      <  1:469676/94‑1 (MQ=255)                                                   cttccgaTCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGtat                                      <  1:1776158/94‑1 (MQ=255)                                                    ttccgaTCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCACTTACCCAACCAGTGCGGTTATTAATAGTACCGtata                                     <  1:2142012/95‑1 (MQ=255)                                                             ttGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACAt                            <  1:3502636/100‑1 (MQ=255)                                                              tGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATc                           >  1:1781007/1‑100 (MQ=255)                                                                gCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCtt                         <  1:1044176/100‑1 (MQ=255)                                                                    cTGATGCAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATGGTACCGTATAAATAAACATCTTTGcc                     <  1:1102051/100‑1 (MQ=255)                                                                     tGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCt                    <  1:74238/100‑1 (MQ=255)                                                                      gATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTa                   >  1:5806626/1‑100 (MQ=255)                                                                      gATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGAATAAATAAACATCTTTGCCTa                   >  1:4574215/1‑100 (MQ=255)                                                                       aTGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATTTTTGCCTAc                  <  1:3890467/100‑1 (MQ=255)                                                                       aTGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCtgg                  <  1:25148/100‑3 (MQ=255)                                                                       aTGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTAc                  <  1:3264391/100‑1 (MQ=255)                                                                       aTGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTAc                  <  1:835145/100‑1 (MQ=255)                                                                       aTGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTAc                  <  1:6003695/100‑1 (MQ=255)                                                                       aTGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTAc                  <  1:6003668/100‑1 (MQ=255)                                                                       aTGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTAc                  <  1:4219200/100‑1 (MQ=255)                                                                       aTGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTAc                  <  1:473/100‑1 (MQ=255)                                                                        tGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTACa                 <  1:4586661/100‑1 (MQ=255)                                                                         gTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTagtt                <  1:4518757/100‑4 (MQ=255)                                                                         gTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTACAg                <  1:3189317/100‑1 (MQ=255)                                                                                 tttCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTACAGATACTTGt        <  1:3223120/100‑1 (MQ=255)                                                                                   tCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTACAGATACTTGttg      <  1:5480846/100‑2 (MQ=255)                                                                                      cagcagTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACTGTATAAATAAACATCTTTGCCTACAGATACTTGTTTCGt   <  1:3420819/100‑1 (MQ=255)                                                                                       aGCAGTTGGTGCAGCTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTACAGAAACTTGTTTCGtt  <  1:5004449/100‑1 (MQ=255)                                                                                          |                                                                                                 TTTGGTGTTACATAGTAATAACCATTACCATTTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCGGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTACAGATACTTGTTTCGTT  >  NC_007793/1045096‑1045281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑