Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,022,294	G→A	intergenic (+171/‑100)	SAUSA300_RS05005 → / → SAUSA300_RS05010	CAAX amino protease/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,022,294	0	G	A	100.0%	99.7 / NA	37	intergenic (+171/‑100)	SAUSA300_RS05005/SAUSA300_RS05010	CAAX amino protease/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/27);  total (10/27)

ATAGTGGTAATATTTTTTAACAGCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGGTATAATATTTTTGAGCAAGTTGAATTGATGGTGGCTATCTGAGTAAAGGGGGGTGGTGCCTATGGCAT  >  NC_007793/1022208‑1022362                                                                                       |                                                                     ctaGTGGTAATATTTTTTAACAGCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTg                                                         <  1:293321/99‑1 (MQ=255)     tGGTAATATTTTTTAACAGCAAGCGGTTATTATTGAGCTTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCa                                                     >  1:909467/1‑100 (MQ=255)     tGGTAATATTTTTTAACAGCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCa                                                     <  1:2454060/100‑1 (MQ=255)        tAATATTTTTTAACAGCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTGATAAAAATGATATAATATTTTTGAGCAAGt                                                  <  1:2138177/100‑1 (MQ=255)                  tAACAGCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGAtg                                        <  1:6454200/100‑1 (MQ=255)                   aaCAGCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGAtgg                                       <  1:2053838/100‑1 (MQ=255)                   aaCAGCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTGTGAGCAAGTTGAATTGAtgg                                       <  1:2244469/100‑1 (MQ=255)                    ccAGCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGAtggt                                      >  1:6434676/2‑100 (MQ=255)                    ccAGCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGAtggt                                      >  1:3901193/2‑100 (MQ=255)                    aCAGCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGAtggg                                      <  1:4198359/100‑2 (MQ=255)                     cAGCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTGCTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGAtggag                                     <  1:1834102/100‑3 (MQ=255)                     cAGCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGAtggtg                                     >  1:3545399/1‑100 (MQ=255)                     cAGCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGAtggtg                                     <  1:407071/100‑1 (MQ=255)                     cAGCAAGAGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGAtggtg                                     >  1:1640648/1‑100 (MQ=255)                      tgCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGAtggtgg                                    >  1:5823188/2‑100 (MQ=255)                       gCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGAtggtgga                                   <  1:3213248/100‑2 (MQ=255)                       gCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGAtggtgag                                   >  1:300506/1‑98 (MQ=255)                       gCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGTGGc                                   <  1:1593476/100‑1 (MQ=255)                       gCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATAGTGGc                                   >  1:3122214/1‑100 (MQ=255)                        cAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGTGGCt                                  <  1:5056125/100‑1 (MQ=255)                        cAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGTGGCt                                  >  1:4460622/1‑100 (MQ=255)                        cAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATA‑TTTTGAGCAAGTTGAATTGATGGTGGCTa                                 <  1:2224281/100‑1 (MQ=255)                         aaGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGTGGCTa                                 >  1:3856777/1‑100 (MQ=255)                             ggTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGTGGCTATCTg                             <  1:3054535/100‑1 (MQ=255)                               ttattaTTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGTGGCTATCTggg                           <  1:2522778/100‑3 (MQ=255)                                      tGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATA‑TTTTGAGCAAGTTGAATTGATGGTGGCTATCTGAGTAAAgggg                   <  1:3228180/100‑1 (MQ=255)                                         gaTTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGGGGCTATCTGAGTAAAgggggg                 <  1:3863443/100‑1 (MQ=255)                                          aTTAGGTTTTAGTCAG‑TTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGTGGCTATCTGAGTAAAGGGGggtg               <  1:4930955/100‑1 (MQ=255)                                           ttAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGTGGCTATCTGAGTAAAGGGGggtg               <  1:5875491/100‑1 (MQ=255)                                           ttAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGTGGCTATCTGAGTAAAGGGGggtg               <  1:2102054/100‑1 (MQ=255)                                               gTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGTGGCTATCTGAGTAAAGGGGGGTGGTGc           <  1:4356584/100‑1 (MQ=255)                                                 ctctgTCAGTTTTTATTACTAAAGATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGTGGCTATCTGAGT‑AAGGGGGGTGGTGCCTa        <  1:6370726/96‑1 (MQ=38)                                                 atctgTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGTGGCTATCTGAGTAAAGGGGGGTGGTGCCt         <  1:4186800/96‑1 (MQ=255)                                                  ttAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGTGGCTATCTGAGTAAAGGGGGGTGGTGCCTa        <  1:5985623/100‑1 (MQ=255)                                                   tcgTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGTGGCTATCTGAGTAAAGGGGGGTGGTGCCTAt       <  1:3733960/98‑1 (MQ=255)                                                   tAGTCAGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGTGGCTATCTGAGTAAAGGGGGGTGGTGCCTag       <  1:3471887/100‑2 (MQ=255)                                                        aGTTTTTATTACTAAATATTTTATAAAAATGATATAATATTTTTGAGCAAGTTGAATTGATGGTGGCTATCTGAGTAAAGGGGGGTGGTGCCTATGGCag  <  1:2483055/100‑2 (MQ=255)                                                                                       |                                                                     ATAGTGGTAATATTTTTTAACAGCAAGCGGTTATTATTGAGATTAGGTTTTAGTCAGTTTTTATTACTAAATATTTTATAAAAATGGTATAATATTTTTGAGCAAGTTGAATTGATGGTGGCTATCTGAGTAAAGGGGGGTGGTGCCTATGGCAT  >  NC_007793/1022208‑1022362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑