Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	979,291	G→A	A366A (GCG→GCA)	SAUSA300_RS04805 →	peptide ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	979,291	0	G	A	100.0%	39.9 / NA	16	A366A (GCG→GCA)	SAUSA300_RS04805	peptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/1);  total (15/1)

CACTAACAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCGCACTGGGACAAAGCTAAAAAAGAGTTAGGTAAAAATGAAGTGACATTTTCAATGAACACAGAAGATACACCAGATGCAAAAATATCTGCTGAATATATC  >  NC_007793/979194‑979390                                                                                                  |                                                                                                    tacTAACAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGcaca                                                                                                   >  1:2989590/2‑100 (MQ=255)  acTAACAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCacac                                                                                                  >  1:1433353/1‑100 (MQ=255)  acTAACAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCacac                                                                                                  >  1:198219/1‑100 (MQ=255)  acTAACAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCacac                                                                                                  >  1:455105/1‑100 (MQ=255)  acTAACAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCacac                                                                                                  >  1:3529383/1‑100 (MQ=255)  acTAACAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCacac                                                                                                  >  1:339663/1‑100 (MQ=255)  acTAACAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAAAAGCAAGAGCacac                                                                                                  >  1:4834281/1‑100 (MQ=255)  acTAACAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGAATATGCGAGTACCATGAATTCGCCTTTAAAATCCAATCCTAAAGAAGCAAGAGCacac                                                                                                  >  1:6518670/1‑100 (MQ=255)    tAACAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAtcggaaga                                                                                                >  1:6365371/1‑95 (MQ=255)     tccAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAtcggaagag                                                                                               >  1:2445811/3‑94 (MQ=255)     taCAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCACACTgg                                                                                               >  1:2338881/2‑100 (MQ=255)     aaCAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCCAGAtcggaagag                                                                                               >  1:5461658/1‑94 (MQ=255)     aaCAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCACACTgg                                                                                               >  1:3061699/1‑100 (MQ=255)     aaCAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCACACTgg                                                                                               >  1:5224831/1‑100 (MQ=255)     aaCAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCACACTgg                                                                                               >  1:2101106/1‑100 (MQ=255)     aaCAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCACACTgg                                                                                               >  1:1572098/1‑100 (MQ=255)      aCAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCACACCggg                                                                                              >  1:2011380/1‑100 (MQ=255)       cAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCACACTGGGa                                                                                             >  1:2125397/1‑100 (MQ=255)        aGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCCAAAGAAGCAAGAGCACACCGGGAc                                                                                            <  1:5273077/100‑1 (MQ=255)         gCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCACACTGGGACa                                                                                           >  1:4481363/1‑100 (MQ=255)                                   tGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCACACTGGGACAAAGCTAAAAAAAAGTTGGGTaagatc                                                                 >  1:4916025/1‑96 (MQ=255)                                                  taGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCACACTGGGACAAAGCTAAAAAAGAGTTAGGTAAAAATGAAGTGACATTTTCa                                                  >  1:654771/2‑100 (MQ=255)                                                             aTTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCACACTGGGACAAAGCTAAAAAAGAGTTAGGTAAAAATGAAGTGACATTTTCAATGAACACaga                                       >  1:640229/1‑100 (MQ=255)                                                                                 ccTAAAGAAGCAAGAGCACACTGGGACAAAGCTAAAAAAGAGTTAGGTAAAAATGAAGTGACATTTTCAATGAACACAGAAGATGCACCAGAtcggaaga                   >  1:5016321/1‑93 (MQ=255)                                                                                  cTAAAGAAGCAAGAGCACACTGGGACAAAGCTAAAAAAGAGTTAGGTAAAAATGAAGTGACATTTTCAATGAACACAGAAGATACACCAGAtcggaagag                  >  1:2938767/1‑92 (MQ=255)                                                                                        aagCAAGAGCACACTGGGACAAAGCTAAAAAAGAGTTAGGTAAAAATGAAGTGACATTTTCAATGAACACAGAAGATACACCAGATGCAAAAATATctgc            >  1:1832141/1‑100 (MQ=255)                                                                                               agCACACTGGGACAAAGCTAAAAAAGAGTTAGGTAAAAATGAAGTGACATTTTCAATGAACACAGAAGATACACCAGCTGCAAAAATATCTGCTGAatat     >  1:350602/1‑100 (MQ=255)                                                                                                  gcACTGGGACAAAGCTAAAAAAGAGTTAGGTAAAAATGAAGTGACATTTTCAATGAACACAGAAGATACACCAGATGCAAAAATATCTGCTGAATATATc  >  1:3858683/1‑100 (MQ=255)                                                                                                  gcACTGGGACAAAGCTAAAAAAGAGTTAGGTAAAAATGAAGTGACATTTTCAATGAACACAGAAGATACACCAGATGCAAAAATATCTGCTGAATATATc  >  1:103702/1‑100 (MQ=255)                                                                                                  gcACTGGGACAAAGCTAAAAAAGAGTTAGGTAAAAATGAAGTGACATTTTCAATGAACACAGAAGATACACCAGATGCAAAAATATCTGCTGAATATATc  >  1:1200847/1‑100 (MQ=255)                                                                                                  |                                                                                                    CACTAACAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCGCACTGGGACAAAGCTAAAAAAGAGTTAGGTAAAAATGAAGTGACATTTTCAATGAACACAGAAGATACACCAGATGCAAAAATATCTGCTGAATATATC  >  NC_007793/979194‑979390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑