Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	967,086	A→G	R223G (AGA→GGA)	SAUSA300_RS04740 →	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	967,086	0	A	G	100.0%	10.9 / NA	7	R223G (AGA→GGA)	SAUSA300_RS04740	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/0);  total (7/0)

GCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACAAAT  >  NC_007793/966996‑967153                                                                                           |                                                                    gCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTgggagatcgga                                                            >  1:1073670/1‑93 (MQ=255)                     ggggTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTGAGAGAGCGGGTAATGCTCCt                                        >  1:4784307/2‑100 (MQ=255)                     aGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTGAGAGAGCGGGTAATGCTCCt                                        >  1:4244176/1‑100 (MQ=255)                     aGGGTTCGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTGAGAGAGCGGGTAATGCTCCt                                        >  1:2240513/1‑100 (MQ=255)                                  gCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAaataa                           >  1:5814372/1‑100 (MQ=255)                                              gCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGa               >  1:1339275/1‑100 (MQ=255)                                                          cctGGTGCGAAGCAAATAGAAACTACATTTTTGGGAATTGGTGTGAGAGCGGGTAATACTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACaaa   >  1:6595714/3‑100 (MQ=255)                                                           gtGGTGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACAAAt  >  1:3687636/2‑100 (MQ=255)                                                                                           |                                                                    GCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACAAAT  >  NC_007793/966996‑967153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑