Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	961,879	C→T	P375L (CCA→CTA)	SAUSA300_RS04725 →	O‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	961,879	0	C	T	100.0%	38.7 / NA	15	P375L (CCA→CTA)	SAUSA300_RS04725	O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/1);  total (14/1)

TGCAGAGCTATCATATCGCTTTATAGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACA  >  NC_007793/961796‑961968                                                                                    |                                                                                          tGCAGAGCTATCATATCGCTTTATAGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATg                                                                           >  1:2577307/1‑100 (MQ=255) tGCAGAGCTATCATACCGCTTTATAGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATg                                                                           >  1:3969628/1‑100 (MQ=255)    agagCTATCATATCGCTTTATAGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCa                                                                        <  1:2602885/100‑1 (MQ=255)          aTCATATCGCTTTATAGAAACTCCATTTAGAAAAGAAGGTATTAACGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTgta                                                                  >  1:6462446/1‑100 (MQ=255)          aTCATATCGCTTTATAGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCGATTgta                                                                  >  1:2886786/1‑100 (MQ=255)          aTCATATCGCTTTATAGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTgta                                                                  >  1:1462372/1‑100 (MQ=255)                            aaCTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCa                                                >  1:2269044/1‑100 (MQ=255)                            aaCTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCa                                                >  1:3812657/1‑100 (MQ=255)                                ccATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTa                                            >  1:1907866/1‑100 (MQ=255)                                ccATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTa                                            >  1:2187692/1‑100 (MQ=255)                                ccATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTa                                            >  1:2952943/1‑100 (MQ=255)                                ccATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTa                                            >  1:3573457/1‑100 (MQ=255)                                                                          tcttATATACTACAATTTATAAGAATGGCAATTGTATTAACCTTGTTAATTCCATTTATTTTGATTTTAGTAGGGGCATTCAATAAATATGGGAAAGACa  >  1:2335476/1‑100 (MQ=255)                                                                          tcttATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACa  >  1:2543688/1‑100 (MQ=255)                                                                          tcttATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACa  >  1:813529/1‑100 (MQ=255)                                                                                    |                                                                                          TGCAGAGCTATCATATCGCTTTATAGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACA  >  NC_007793/961796‑961968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑