Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	952,565	A→C	K194T (AAA→ACA)	SAUSA300_RS04695 →	ATP‑dependent helicase/nuclease subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	952,565	0	A	C	100.0%	44.7 / NA	20	K194T (AAA→ACA)	SAUSA300_RS04695	ATP‑dependent helicase/nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/0);  total (20/0)

GAATTAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTAAAAATTGGTTGGATCAATTGGTGACACCATACGAAGAAGAAGCACAACAAGCGCAACTTATTCAACTACTAACAGACTTATCTAAAGTATTT  >  NC_007793/952468‑952656                                                                                                  |                                                                                            gAATTAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTacaa                                                                                           >  1:316747/1‑100 (MQ=255)     tAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAAttg                                                                                       >  1:437333/1‑100 (MQ=255)     tAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAAttg                                                                                       >  1:2691308/1‑100 (MQ=255)      aaCAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAAttgg                                                                                      >  1:2868244/1‑100 (MQ=255)       aCAGAGCAATTGTCTTCAGGTAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAAttggt                                                                                     >  1:1061362/1‑100 (MQ=255)              aaTTGTCTTCAGATAGAAGAGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCa                                                                              >  1:3231819/1‑100 (MQ=255)                 tGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAAtt                                                                           >  1:4486172/1‑100 (MQ=255)                   tggccAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTgg                                                                         >  1:4258463/5‑100 (MQ=255)                      ccAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTgc                                                                      >  1:1397234/2‑99 (MQ=255)                       cAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTAac                                                                     >  1:2405941/1‑100 (MQ=255)                              taGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCAtgc                                                              >  1:2802739/2‑98 (MQ=255)                              taGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCAtgc                                                              >  1:6555326/2‑98 (MQ=255)                               aGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCATACg                                                             >  1:5730205/1‑100 (MQ=255)                                     taccAGCTTCGAATGATTATTAAACAATAGTATTTCTTTAGCGTTGCAAATCCAAATCCAACAAATTGGTTGGATCAATTGGTGACACCATAAgagatcg                                                       >  1:3567096/4‑95 (MQ=255)                                     gatCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCaagatcggaag                                                       >  1:6100837/1‑90 (MQ=255)                                     gatCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGATGGATCAATTGGTGACACCGTACggagatc                                                       >  1:5697581/1‑98 (MQ=255)                                        cAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCGTACgaagatcgga                                                    >  1:4092483/1‑95 (MQ=255)                                        cAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCATAcagatcggaag                                                    >  1:6558071/1‑90 (MQ=255)                                        cAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCATACgaagatcgga                                                    >  1:5248989/1‑95 (MQ=255)                                        cAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCATACGaagaagaag                                                    >  1:2261317/1‑100 (MQ=255)                                                                                          aaatccTACAAATTGGTTGGATCAATTGGTGACACCATACGAAGAAGAAGCACAACAAGCGCAACTTATTCAACTACTAACAGACTTATCTAAAGTAttt  >  1:5207881/1‑100 (MQ=255)                                                                                                  |                                                                                            GAATTAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTAAAAATTGGTTGGATCAATTGGTGACACCATACGAAGAAGAAGCACAACAAGCGCAACTTATTCAACTACTAACAGACTTATCTAAAGTATTT  >  NC_007793/952468‑952656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑