Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	919,052	A→G	intergenic (‑41/‑60)	SAUSA300_RS04535 ← / → SAUSA300_RS04540	hypothetical protein/disulfide oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	919,052	0	A	G	100.0%	42.5 / NA	18	intergenic (‑41/‑60)	SAUSA300_RS04535/SAUSA300_RS04540	hypothetical protein/disulfide oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/14);  total (4/14)

AAGACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTTTATTAGTATATTATAATAGATAGTGTAATAAAAATCTATCAAACCAATGGGGGATAAATATATGGAGCACGTGAGTGTGGTAGTTTATGGGGCA  >  NC_007793/918955‑919144                                                                                                  |                                                                                             aaGACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAGTCTttgtt                                                                                            <  1:1320127/100‑1 (MQ=255) aaGACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgtt                                                                                            <  1:5827960/100‑1 (MQ=255) aaGACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgtt                                                                                            <  1:2885478/100‑1 (MQ=255) aaGACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgtt                                                                                            <  1:381521/100‑1 (MQ=255) aaGACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgtt                                                                                            <  1:2163628/100‑1 (MQ=255) aaGACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgtt                                                                                            <  1:2081459/100‑1 (MQ=255) aaGACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgtt                                                                                            <  1:649364/100‑1 (MQ=255) aaGACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgtt                                                                                            <  1:4730337/100‑1 (MQ=255) aaGACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgtt                                                                                            <  1:735585/100‑1 (MQ=255) aaGACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgtt                                                                                            <  1:1542808/100‑1 (MQ=255) aaGACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgtt                                                                                            <  1:535081/100‑1 (MQ=255) aaGACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgtt                                                                                            <  1:6024227/100‑1 (MQ=255) aaGACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCCTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgtt                                                                                            <  1:1682431/100‑1 (MQ=255) aaGACGTTCAATAACTGCTGCTGCTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgtt                                                                                            <  1:5044031/100‑1 (MQ=255)  aGACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGTATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgttg                                                                                           <  1:3076537/100‑2 (MQ=255)   gaagTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgttgt                                                                                          <  1:4405314/97‑3 (MQ=255)   gACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTGGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgttgt                                                                                          <  1:949919/100‑3 (MQ=255)   gACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgttgt                                                                                          <  1:3329137/100‑3 (MQ=255)   gACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgttgt                                                                                          <  1:3596678/100‑3 (MQ=255)   gACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgttgt                                                                                          <  1:5623425/100‑3 (MQ=255)   gACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgttgt                                                                                          <  1:5123741/100‑3 (MQ=255)   gACGTTCAATAACTCCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgttgt                                                                                          <  1:4968946/100‑3 (MQ=255)    aCGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgttgtg                                                                                         <  1:6159884/100‑4 (MQ=255)    aCGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgttgtg                                                                                         <  1:1933734/100‑4 (MQ=255)    aCGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgttgta                                                                                         <  1:3653002/100‑4 (MQ=255)    aCGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgttgta                                                                                         <  1:1560292/100‑4 (MQ=255)    aCGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGAATCAGGCAAGTCTCCTTTCGAGAGATGAAGTTGATAATCTttgttgga                                                                                         <  1:3095354/100‑4 (MQ=255)    aCGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCATCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTttgttgtc                                                                                         <  1:810018/100‑4 (MQ=255)      gTTCAATACCTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTTTGTTAGtat                                                                                       <  1:3131028/100‑1 (MQ=255)      gTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTTTGTTAGtat                                                                                       <  1:6179105/100‑1 (MQ=255)              aCTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTTTGTTAGTATATTataat                                                                               <  1:6095001/100‑1 (MQ=255)              aCTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTTTGTTAGTATATTataat                                                                               <  1:582006/100‑1 (MQ=255)                       aCTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTTTGTTAGTATATTATAATAGATAgtgt                                                                      <  1:6062628/100‑1 (MQ=255)                                               tCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTTTGTTAGTATATTATAATAGTTTGTGTAATAAAAATCTATCAAACCAATgg                                              >  1:2539070/1‑100 (MQ=255)                                                  ttAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTTTGTTAGTATATTATAATAGATAGTGTAATAAAAATCTATCAAACCAATgggga                                           >  1:1264357/2‑99 (MQ=255)                                                  ctAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTTTGTTAGTATATTATAATAGATAGTGTAATAAAAATCTATCAAACCAATgggag                                           >  1:1162301/2‑98 (MQ=255)                                                    aGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTTTGTTAGTATATTATAATAGATAGTGTAATAAAAATCTATCAAACCAAAGGGGGag                                         >  1:6296432/1‑99 (MQ=255)                                                              cAAGTCTCCTTTCGAGAGATGAATTTGATAATCTTTGTTAGTATATTATAATAGATAGTGTAATAAAAATCTATCAAACCAATGGGGGATAAATATATgg                               <  1:2021740/100‑1 (MQ=255)                                                              cAAGTCTCCTTTCGAGAGATGAATTTGATAATCTTTGTTAGTATATTATAATAGATAGTGTAATAAAAATCTATCAAACCAATGGGGGATAAATATATgg                               <  1:172379/100‑1 (MQ=255)                                                                      cTTTCGAGAGATGAATTTGATAATCTTTGTTAGTATATTATAATAGATAGTGTAATAAAAATCTATCAAACCAATGGGGGATAAATATATGGAGCACGTg                       <  1:4158979/100‑1 (MQ=255)                                                                      cTTTCGAGAGATGAATTTAATAATCTTTGTTAGTATATTATAATAGATAGTGTAATAAAAATCTATCAAACCAATGGGGGATAAATATATGGAGCACGTg                       <  1:2065872/100‑1 (MQ=255)                                                                            agagaTGAATTTGATAATCTTTGTTAGTATATTATAATAGATAGTGTAATAAAAATCTATCAAACCAATGGGGGATAAATATATGGAGCACGTGAgtcac                 <  1:553343/100‑4 (MQ=255)                                                                                   aaTTTGATAATCTTTGTTAGTATATTATAATAGATAGTGTAATAAAAATCTATCAAACCAATGGGGGATAAATATATGGAGCACGTGAGTGTGGTAGttt          <  1:1090636/100‑1 (MQ=255)                                                                                          tAATCTTTGTTAGTATATTATAATAGATAGTGTAATAAAAATCTATCAAACCAATGGGGGAGAAATATATGGAGCACGTGAGTGTGGTAGTTTATGGGGc   <  1:1412725/100‑1 (MQ=255)                                                                                          tAATCTTTGTTAGCATATTATAATAGATAGTGTAATAAAAATCTATCAAACCAATGGGGGATAAATATATGGAGCACGTGAGTGTGGTAGTTTATGGGGc   <  1:4348125/100‑1 (MQ=255)                                                                                           aaTCTTTGTTAGTATATTATAATAGATAGTGTAATAAAAATCTATCAAACCAATGGGGGATAAATATATGGAGCACGTGAGTGTGGTAGTTTATGGGGCa  <  1:5699781/100‑1 (MQ=255)                                                                                                  |                                                                                             AAGACGTTCAATAACTTCTGCTACTTGATCAAACATCGTTGTATCTTCAGTAGGCATCAGGCAAGTCTCCTTTCGAGAGATGAATTTGATAATCTTTATTAGTATATTATAATAGATAGTGTAATAAAAATCTATCAAACCAATGGGGGATAAATATATGGAGCACGTGAGTGTGGTAGTTTATGGGGCA  >  NC_007793/918955‑919144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑