Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	906,507	A→C	T6T (ACA→ACC)	SAUSA300_RS04455 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	906,507	0	A	C	100.0%	77.0 / NA	30	T6T (ACA→ACC)	SAUSA300_RS04455	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/3);  total (27/3)

TGCAATATAAATAATCGACCGCAATCCACAAAAGCACAAGCACCCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACAAATTTAATCAAACGAAAACATCAAG‑CTG‑A‑AGATCGCCGAAAGATTTTAATCAAGCAAAAACATCAAACTAAAGTTCGCTGAAATGATTATGATA  >  NC_007793/906410‑906601                                                                                                  |                                                                                                  tGCAATATAAATAATCGACCGCAATCCACAAAAGCACAAGCACCCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACcaa                                                                                                 >  1:3429410/1‑100 (MQ=255)  gCAATATAAATAATCGACCGCAATCCACACAAGCACAAGCACCCCCAAACATTACTTTACTGCTTGCCATTTTTGAGGATTGCGTTTCTATTTTACCAAt                                                                                                >  1:1697247/1‑100 (MQ=255)  gCAATATAAATAATCGACCGCAATCCACAAAAGCACAAGCACCCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAAt                                                                                                >  1:109955/1‑100 (MQ=255)  gCAATATAAATAATCGACCGCAATCCACAAAAGCACAAGCACCCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAAt                                                                                                >  1:6244901/1‑100 (MQ=255)  gCAATATAAATAATCGACCGCAATCCACAAAAGCACAAGCACCCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAAt                                                                                                >  1:1957811/1‑100 (MQ=255)  gCAATATAAATAATCGACCGCAATCCACAAAAGCACAAGCACCCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAAt                                                                                                >  1:2128826/1‑100 (MQ=255)                            acaAAAGCACAAGCACCCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTgc                                                                     <  1:2455077/100‑2 (MQ=255)                            acaAAAGCACAAGCACCCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTG‑a                                                                    <  1:2783112/100‑1 (MQ=255)                               aaaGCACAAGCACCCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAA‑CTA‑A‑AGa                                                                >  1:6440923/1‑100 (MQ=255)                                       agcacCCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTG‑A‑AGATCGCCGaa                                                        <  1:2883556/100‑1 (MQ=255)                                        gcacCCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTG‑A‑AGATCGCCGaaa                                                       >  1:6396652/1‑100 (MQ=255)                                        acccccccAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCATG‑CTG‑A‑AGATCggaagag                                                       >  1:5976578/4‑94 (MQ=255)                                         cacCCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTG‑ACAGATCggaagag                                                       >  1:2745739/1‑94 (MQ=255)                                         accacccAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTGAA‑AGATCggaagag                                                       >  1:108180/5‑94 (MQ=255)                                          gcccccAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTG‑A‑AGATCGCCGAAAGa                                                     >  1:328285/2‑100 (MQ=255)                                          gcccccAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGCTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTG‑A‑AGATCggaagagca                                                     >  1:4262279/2‑92 (MQ=255)                                          acCCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAGCCTG‑A‑AGATCggaagagc                                                      >  1:1741447/1‑93 (MQ=255)                                          acCCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTGAA‑AGATCggaagagc                                                      >  1:4980337/1‑93 (MQ=255)                                          acCCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CGG‑A‑AGATCggaagagca                                                     >  1:1320573/1‑92 (MQ=255)                                           cccccAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAGG‑CTG‑A‑AGATCggaagagcac                                                    >  1:572487/1‑91 (MQ=255)                                            ccccAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTG‑A‑AGATCggaagagcaca                                                   >  1:3952133/1‑90 (MQ=255)                                             cccAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTGTA‑AGATCggaagagcaca                                                   >  1:5952182/1‑90 (MQ=255)                                             cccAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTG‑A‑AGATCGCCGGAAGAtcg                                                  >  1:3260162/1‑98 (MQ=255)                                              ccAAACATTATTTTAGTGCTTGCAATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGCAAACATCAAG‑CTG‑A‑AGCTCGCCGAAAGAtttt                                                 >  1:1327975/1‑100 (MQ=255)                                                                  tGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTG‑A‑AGATCGCCGAAAGATTTTAATCAAGCAAAAAGATcgga                             >  1:6561148/1‑97 (MQ=255)                                                                  tGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTG‑A‑AGATCGCCGAAAGATTTTAATCAAGCAAAAACATCaaa                             >  1:4126915/1‑100 (MQ=255)                                                                  tGCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTG‑A‑AGATCGCCGAAAGATTTTAATCAAGCAAAAACATCaaa                             >  1:6500537/1‑100 (MQ=255)                                                                   gCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTG‑A‑AGATCGCCGAAAGATTTTAATCAAGCAAAAACATCAAAc                            >  1:3014671/1‑100 (MQ=255)                                                                   gCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTG‑A‑AGATCGCCGAAAGATTTTAATCAAGCAAAAACATCAAAc                            >  1:425791/1‑100 (MQ=255)                                                                   gCCATTTTTGTGGATTGCGTTTCTATTTTACCAATTTAATCAAACGAAAACATCAAG‑CTG‑A‑AGATCGCCGAAAGATTTTAATCAAGCAAAAACATCAAAc                            >  1:2669707/1‑100 (MQ=255)                                                                                             tttACCAATTTAATCAAACGAAAACATCAAG‑CTG‑A‑AGATCGCCGAAAGATTTTAATCAAGCAAAAACATCAAACTAAAGTTCGCTGAAATGATTATGATa  >  1:969128/1‑100 (MQ=255)                                                                                                  |                                                                                                  TGCAATATAAATAATCGACCGCAATCCACAAAAGCACAAGCACCCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTTTACAAATTTAATCAAACGAAAACATCAAG‑CTG‑A‑AGATCGCCGAAAGATTTTAATCAAGCAAAAACATCAAACTAAAGTTCGCTGAAATGATTATGATA  >  NC_007793/906410‑906601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑