Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	903,330	A→G	intergenic (+37/‑153)	SAUSA300_RS04430 → / → SAUSA300_RS04435	Fe‑S cluster assembly protein SufB/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	903,330	0	A	G	100.0%	21.4 / NA	11	intergenic (+37/‑153)	SAUSA300_RS04430/SAUSA300_RS04435	Fe‑S cluster assembly protein SufB/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/0);  total (11/0)

GAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAAT  >  NC_007793/903251‑903362                                                                                |                                 gAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCTGGAAATGGCTATTGATACCAtt              >  1:1010545/1‑100 (MQ=255) gAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGGAAATGGCTATTGATACCAtt              >  1:140046/1‑100 (MQ=255) caaCCGTTTAATCAAGTTCGAAATGGAAGGAAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATCCTTGACTTCCGGAAATGGCTATTGATACCAtt              >  1:5658673/2‑100 (MQ=255)          aaTCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGGAAATGGCTATTGATACCATTTTGATAcca     >  1:6551384/1‑98 (MQ=255)          aaTCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGGAAATGGCTATTGATACCATTTTGATAcca     >  1:6590001/1‑98 (MQ=255)           ctCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGGAAATGGCTATTGATACCATTTTGATAccat    >  1:5494929/2‑97 (MQ=255)            gcAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGGAAATGGCTATTGATACCATTTTGATAccatt   >  1:5978284/2‑96 (MQ=255)             cAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGGAAATGGCTATTGATACCATTTTGATAccattt  >  1:1353585/1‑95 (MQ=255)             cAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGGAAATGGCTATTGATACCATTTTGATAccattt  >  1:3096856/1‑95 (MQ=255)             cAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGGAAATGGCTATTGATACCATTTTGATAccattt  >  1:765368/1‑95 (MQ=255)             cAAGTTCGAAATGGAAGGAAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGGAAATGGCTATTGATACCATTTTGATAccattt  >  1:3899737/1‑95 (MQ=255)                                                                                |                                 GAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAAT  >  NC_007793/903251‑903362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑