Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	895,570	G→A	intergenic (‑441/‑593)	SAUSA300_RS04395 ← / → SAUSA300_RS04400	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	895,570	0	G	A	100.0%	24.6 / NA	11	intergenic (‑441/‑593)	SAUSA300_RS04395/SAUSA300_RS04400	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/0);  total (11/0)

GGCCATATATCTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTCATATACATATATCTTGATAACATAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTT  >  NC_007793/895474‑895599                                                                                                 |                              ggCCATATATCTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTAcaccac                           >  1:588262/1‑100 (MQ=38)         atCTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTa                   >  1:3826145/1‑100 (MQ=38)           ttGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGt                 >  1:756680/2‑100 (MQ=38)           cTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGt                 >  1:3908686/1‑100 (MQ=38)           cTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGt                 >  1:6419081/1‑100 (MQ=38)             ggCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGTTa               >  1:2320566/1‑100 (MQ=38)             ggCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGTTa               >  1:49241/1‑100 (MQ=38)                         cacaGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGTTACTGGGTGgttgt   >  1:2657562/1‑100 (MQ=38)                         cacaGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGTTACTGGGTGgttgt   >  1:4920394/1‑100 (MQ=38)                         cacaGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGTTACTGGGTGgttgt   >  1:5210950/1‑100 (MQ=38)                         cacaGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGTTACTGGGTGgttgt   >  1:6558881/1‑100 (MQ=38)                          ccaGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGTTACTGGGTGgttgtt  >  1:1433381/2‑100 (MQ=38)                                                                                                 |                              GGCCATATATCTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTCATATACATATATCTTGATAACATAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTT  >  NC_007793/895474‑895599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑