Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	895,560	T→C	intergenic (‑431/‑603)	SAUSA300_RS04395 ← / → SAUSA300_RS04400	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	895,560	0	T	C	100.0%	48.7 / NA	20	intergenic (‑431/‑603)	SAUSA300_RS04395/SAUSA300_RS04400	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/0);  total (20/0)

TAGCCCGGCAGAGGCCATATATCTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTCATATACATATATCTTGATAACATAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTT  >  NC_007793/895462‑895599                                                                                                   |                                        taGCCCGGCAGAGGCCATATATCTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAg                                       >  1:3651786/1‑100 (MQ=255) taGCCCGGCAGAGGCCATATATCTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAg                                       >  1:3811494/1‑100 (MQ=255)  aGCCCGGCAGAGGCCATATATCTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGt                                      >  1:2001552/1‑100 (MQ=255)  aGCCCGGCAGAGGCCATATATCTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGt                                      >  1:2250582/1‑100 (MQ=255)   gCCCGGCAGAGGCCATATATCTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTCGTTACAgat                                     >  1:239356/1‑98 (MQ=255)     ccGGCAGAGGCCATATATCTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGc                                   >  1:5382246/1‑100 (MQ=39)       tgCAGAGGCCATATATCTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTa                                 >  1:3799707/2‑100 (MQ=38)          tgagGCCATATATCTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTAcac                              >  1:4559055/2‑98 (MQ=38)             ggCCATATATCTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTAcaccac                           >  1:588262/1‑100 (MQ=38)                     atCTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTa                   >  1:3826145/1‑100 (MQ=38)                       ttGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGt                 >  1:756680/2‑100 (MQ=38)                       cTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGt                 >  1:3908686/1‑100 (MQ=38)                       cTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGt                 >  1:6419081/1‑100 (MQ=38)                         ggCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGTTa               >  1:49241/1‑100 (MQ=38)                         ggCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGTTa               >  1:2320566/1‑100 (MQ=38)                                     cacaGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGTTACTGGGTGgttgt   >  1:4920394/1‑100 (MQ=38)                                     cacaGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGTTACTGGGTGgttgt   >  1:5210950/1‑100 (MQ=38)                                     cacaGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGTTACTGGGTGgttgt   >  1:2657562/1‑100 (MQ=38)                                     cacaGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGTTACTGGGTGgttgt   >  1:6558881/1‑100 (MQ=38)                                      ccaGGAGACATCTTCCTTGTCATCACTC‑GATACATATATCTTGATAACATAGAGTTGTTACAGTCGCTACACCACTCATACTAGTTACTGGGTGgttgtt  >  1:1433381/2‑100 (MQ=38)                                                                                                   |                                        TAGCCCGGCAGAGGCCATATATCTGGCTGTTGGTCTCACAGGAGACATCTTCCTTGTCATCACTCATATACATATATCTTGATAACATAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTT  >  NC_007793/895462‑895599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑