Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	95,813	T→C	pseudogene (253/1194 nt)	SAUSA300_RS00450 →	pyridine nucleotide‑disulfide oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	95,813	0	T	C	100.0%	13.8 / NA	7	pseudogene (253/1194 nt)	SAUSA300_RS00450	pyridine nucleotide‑disulfide oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/3);  total (4/3)

ATCTAGTTTGAAAAGTTCTCGTAAAGATATGGAAAGTGTTATACCTGAAGGTGCTAACTGGATAAAACAGGCTGTTTCAAGTTTTTAACCTGAAAATAATAGCGTTATTTTAGGAGATAATACAGTCGTTTATTATGATTTTTTAGTAGTAGCTCCAGGATTACAGATT  >  NC_007793/95728‑95896                                                                                      |                                                                                    aTCTAGTTTGAAAAGTTCTCGTAAAGATATGGAAAGTGTTATACCTGAAGGTTCTAACTGGATAAAACACGCTGTTTCAAGTTTTCAACCTGAAaataat                                                                       >  1:6235585/1‑100 (MQ=255)         tGAAAAGTTCTCGTAAAGATATGGAAAGTGTTATACCTGAAGGTGCTAACTGGATAAAACAGGCTGTTTCAAGTTTTCAACCTGAAAATAATAGCGttga                                                               <  1:214642/100‑3 (MQ=255)                    cGTAAAGATATGGAAAGTGTTATACCTGAAGGTGCTAACTGGATAAAACAGGCTGCTTCAAGTTTTCAACCTGAAAATAATAGCGTTATTTTAGGAGata                                                    >  1:4646892/1‑100 (MQ=255)                        cctgtatGGAAAGTGTTATACCTGAAGGTGCTAACTGGATAAAACAGGCTGTTTCAAGTTTTCAACCTGAAAATAATAGCGTTATTTTAGGAGATAATAc                                                >  1:4944582/5‑100 (MQ=255)                                                                 aaaCAGGCTGTTTCAAGTTTTCAACCTGAAAATAATAGCGTTATTTTAGGAGATAATACAGTCGTTTATTATGATTTTTTAGTAGTAGCTCCAGGATTAc       <  1:2185226/100‑1 (MQ=255)                                                                  aaCAGGCTGTTTCAAGTTTTCAACCTGAAAATAATAGCGTTATTTTAGGAGATAATACAGTCGTTTATTATGATTTTTTAGTAGTAGCTTCAgagatcgg      >  1:5782804/1‑93 (MQ=255)                                                                      ggCTGTTTCAAGTTTTCAACCTGAAAATAATAGCGTTATTTTAGGAGATAATACAGTCGTTTATTATGATTTTTTAGTAGTAGCTCCAGGATTACAGAtt  <  1:5713436/100‑1 (MQ=255)                                                                                      |                                                                                    ATCTAGTTTGAAAAGTTCTCGTAAAGATATGGAAAGTGTTATACCTGAAGGTGCTAACTGGATAAAACAGGCTGTTTCAAGTTTTTAACCTGAAAATAATAGCGTTATTTTAGGAGATAATACAGTCGTTTATTATGATTTTTTAGTAGTAGCTCCAGGATTACAGATT  >  NC_007793/95728‑95896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑