Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	877,041	G→T	E96* (GAA→TAA)	SAUSA300_RS04280 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	877,041	0	G	T	100.0%	63.0 / NA	24	E96* (GAA→TAA)	SAUSA300_RS04280	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/1);  total (23/1)

CAATGGTATTGCTAGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAAGAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTATGGAATGTCTGAAAATTTAAAGGGAAACAATATAAACTCAAGGCTTG  >  NC_007793/876973‑877131                                                                     |                                                                                           cAATGGTATTGCTAGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTCTGGATTAGTTCaga                                                             >  1:2057470/1‑100 (MQ=255)           gCTAGCACAGATTAATTTGGATGATCTTCCCGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATg                                                   >  1:6594038/1‑100 (MQ=255)            ttAGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGt                                                  >  1:1412259/2‑100 (MQ=255)            atAGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGt                                                  >  1:150181/2‑100 (MQ=255)            acaGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTTCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGt                                                  >  1:1342159/3‑100 (MQ=255)             tAGCACAGATTACTTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTa                                                 >  1:3356457/1‑100 (MQ=255)             tAGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTa                                                 >  1:1592339/1‑100 (MQ=255)             tAGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTa                                                 >  1:5995497/1‑100 (MQ=255)             tAGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTa                                                 >  1:3249899/1‑100 (MQ=255)             tAGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTa                                                 >  1:4103707/1‑100 (MQ=255)             tAGCACAGATTAATTTGGATGATCTTCCAAAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTa                                                 >  1:5990216/1‑100 (MQ=255)              aGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTAt                                                >  1:1103390/1‑100 (MQ=255)              aGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTAt                                                >  1:5635818/1‑100 (MQ=255)              aGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTAt                                                >  1:4449912/1‑100 (MQ=255)              aGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTAt                                                >  1:4123027/1‑100 (MQ=255)              aGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTAt                                                >  1:3291517/1‑100 (MQ=255)              aGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTAt                                                >  1:2551727/1‑100 (MQ=255)               ctgcaGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTaag                                               >  1:151125/4‑98 (MQ=255)                ctcaGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTATgg                                              >  1:4428806/3‑100 (MQ=255)                 ccaGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTATGGa                                             >  1:1941145/2‑100 (MQ=255)                 ccaGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTATGGa                                             >  1:6031939/2‑100 (MQ=255)                  caGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTATGGaa                                            >  1:5618275/1‑100 (MQ=255)                                      tCCAGAACAACAAGAATTATTTCCTGTAAAATAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTATGGAATGTCTGAAAATTTAAAgggt                        <  1:1698584/100‑2 (MQ=255)                                                            ccTGTAAAATAAGGGATATTGCAGTTTTGGACTAGTTCAGAAGATCAAATGTATGGAATGTCTGAAAATTTAGAGGGAAACAATATAAACTCAAgatcgg  >  1:233325/1‑95 (MQ=255)                                                                     |                                                                                           CAATGGTATTGCTAGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGAATTATTTCCTGTAAAAGAAGGGATATTGCAGTTTTGGATTAGTTCAGAAGATCAAATGTATGGAATGTCTGAAAATTTAAAGGGAAACAATATAAACTCAAGGCTTG  >  NC_007793/876973‑877131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑