Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	876,937	A→C	Y61S (TAT→TCT)	SAUSA300_RS04280 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	876,937	0	A	C	100.0%	40.3 / NA	17	Y61S (TAT→TCT)	SAUSA300_RS04280	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (16/1);  total (16/1)

CAGTACTACCATGTATTGAAATGGATCTAAAAGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATATTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGCTAGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGA  >  NC_007793/876847‑877024                                                                                           |                                                                                        cAGTACTACCATGTATTGAAATGGATCTAAAAGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGa                                                                                >  1:4334509/1‑100 (MQ=255)        acCATGTATTGGAATGGATCTAAAAGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCa                                                                         >  1:6507401/1‑100 (MQ=255)        acCATGTATTGAAATGGATCTAAAAGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCa                                                                         >  1:5275327/1‑100 (MQ=255)         ccATGTATTGAAATGGATCTAAAAGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCAt                                                                        <  1:5483104/100‑1 (MQ=255)                             aaaaGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCa                                                    >  1:3584854/1‑100 (MQ=255)                             aaaaGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCa                                                    >  1:2566948/1‑100 (MQ=255)                             aaaaGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCa                                                    >  1:2371372/1‑100 (MQ=255)                                 ccgaTGTAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCAATgg                                                >  1:5953581/3‑100 (MQ=255)                                    aTGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAACGTACCagatcgga                                             >  1:3298508/1‑93 (MQ=255)                                                    tAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGCCAGCACAGAtcgg                             >  1:2039927/1‑97 (MQ=255)                                                     aaaCCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGCTAGCACAGATTAAt                            >  1:2451378/1‑100 (MQ=255)                                                                attttGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGCTAGCACAGATTAATTTGGATGATCt                 >  1:3765888/4‑100 (MQ=255)                                                                   atggtggAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGCTAGCACAGATTAATTTGGATGATCTTcc              >  1:1114703/2‑100 (MQ=255)                                                                    tggtggAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGCTAGCACAGATTAATTTGGATGATCTTCCa             >  1:5357151/1‑100 (MQ=255)                                                                     ggtggAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGCTAGCACAGATTAATTTGGATGATCTTCCAg            >  1:4492592/1‑100 (MQ=255)                                                                         gtaTGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGCTAGCACAGATTAATTTGGATGATCTTCCAGaaca        >  1:284993/3‑100 (MQ=255)                                                                               cATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGCTAGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGa  >  1:1197819/1‑100 (MQ=255)                                                                                           |                                                                                        CAGTACTACCATGTATTGAAATGGATCTAAAAGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATATTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGCTAGCACAGATTAATTTGGATGATCTTCCAGAACAACAAGA  >  NC_007793/876847‑877024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑