Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	867,908	T→C	intergenic (+450/+46)	SAUSA300_RS04190 → / ← SAUSA300_RS04195	cold‑shock protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	867,908	0	T	C	100.0%	85.3 / NA	32	intergenic (+450/+46)	SAUSA300_RS04190/SAUSA300_RS04195	cold‑shock protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/31);  total (1/31)

AATCAACTTTTTGTTAATTTTTTATTAAGACTGATTAATTATTGAGAATATTTATTGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTTTTATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCTACTG  >  NC_007793/867814‑868003                                                                                               |                                                                                                aaTCAACTTTTTGTTAATTTTTTATTAAGACTGATTAATTATTGAGAATATTTATTGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTttcattta                                                                                            >  1:4848901/1‑100 (MQ=255) aaTCAACTTTTTGTTAATTTTTTATTAAGACTGATTAATTATTGAGAATATTTATTGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTttcattta                                                                                            >  1:654115/1‑100 (MQ=255)    cAACTTTTTGTTAATTTTTTATTAAGACTGATTAATTATTGAGAATATTTATTGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTTTCATTTaaaa                                                                                         >  1:6591602/1‑100 (MQ=255)         ttttGTTAATTTTTTATTAAGACTGATTAATTATTGAGAATATTTATTGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTTTCATTTAAAAGGCgt                                                                                    <  1:1631919/100‑2 (MQ=255)          tttGTTAATTTTTTATTAAGACTGATTAATCATTGAGAATATTTATTGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTTTCATTTAAAAGGCGaa                                                                                   <  1:1545679/100‑1 (MQ=255)                                         attGAGAATATTTATTGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGcag                                                    <  1:1972959/100‑3 (MQ=255)                                         attGAGAATATTTATTGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGt                                                    <  1:352560/100‑1 (MQ=255)                                           tGAGAATAGTTATTGTTTTTAAAATAGCATAATAATTCAGGAATCTTGTGGTCAGGTAAAAGGCGAAACAGGAAAATAATTAAATAAAAATATTGCGtag                                                  <  1:4127985/100‑3 (MQ=255)                                            gagaATATTTATTGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTa                                                 <  1:3921972/100‑1 (MQ=255)                                                          ctcttccgaTCTCATACTAATTCAGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCaa                                   <  1:5075557/92‑1 (MQ=255)                                                                   tctcATAATAATTCAGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTa                          <  1:3724587/100‑1 (MQ=255)                                                                                 tccgaTCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTg            <  1:2578147/96‑1 (MQ=255)                                                                                 tccgaTCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTg            <  1:193078/96‑1 (MQ=255)                                                                                 aGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTg            <  1:3379483/100‑1 (MQ=255)                                                                                 aGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTg            <  1:6104667/100‑1 (MQ=255)                                                                                 aGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTg            <  1:4149146/100‑1 (MQ=255)                                                                                 aGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTg            <  1:4400791/100‑1 (MQ=255)                                                                                 aGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTg            <  1:5561868/100‑1 (MQ=255)                                                                                 aGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTAATTTCTAATGCTTTg            <  1:6089120/100‑1 (MQ=255)                                                                                    gaTCTTGTATTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTgggg         <  1:5071706/99‑1 (MQ=255)                                                                                     aTCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGt        <  1:689595/100‑1 (MQ=255)                                                                                     aTCTTGTTTCCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTgggga        <  1:1416752/100‑2 (MQ=255)                                                                                     aTCTTGTTCTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGt        <  1:1754466/100‑1 (MQ=255)                                                                                      tCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTgggggc       <  1:4900588/100‑3 (MQ=255)                                                                                      tCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTc       <  1:6422312/100‑1 (MQ=255)                                                                                      tCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTc       <  1:6277085/100‑1 (MQ=255)                                                                                       tttGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCt      <  1:6217416/99‑1 (MQ=255)                                                                                       cctGTTTACATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCt      <  1:1964192/98‑1 (MQ=255)                                                                                       cTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCt      <  1:6238229/100‑1 (MQ=255)                                                                                       cTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCt      <  1:728447/100‑1 (MQ=255)                                                                                       cTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCt      <  1:5994026/100‑1 (MQ=255)                                                                                       cTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCt      <  1:518622/100‑1 (MQ=255)                                                                                       cTTGTTTCCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAACGTCAAATATGCTTATTTCTAATGCTTTGGGGttg      <  1:6432300/100‑3 (MQ=255)                                                                                          tgtttcATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCTACt   >  1:3886672/3‑100 (MQ=255)                                                                                          gTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCTaag   <  1:450462/100‑3 (MQ=255)                                                                                           ttttcATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCTACtt  <  1:2330971/100‑2 (MQ=255)                                                                                           ttttcATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCTACTg  <  1:2256627/100‑1 (MQ=255)                                                                                           ttttcATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCTACTg  <  1:2240486/100‑1 (MQ=255)                                                                                               |                                                                                                AATCAACTTTTTGTTAATTTTTTATTAAGACTGATTAATTATTGAGAATATTTATTGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTTTTATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCTACTG  >  NC_007793/867814‑868003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑