Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	853,311	G→A	E601K (GAA→AAA)	SAUSA300_RS04120 →	ribonuclease R

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	853,311	0	G	A	100.0%	99.2 / NA	37	E601K (GAA→AAA)	SAUSA300_RS04120	ribonuclease R
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/2);  total (35/2)

TAATCCGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTGAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGAAAAAAGCAGAATATATGATTCAACATATTGGTGAT  >  NC_007793/853231‑853406                                                                                 |                                                                                                tAATCCGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGa                                                                              >  1:4909634/1‑100 (MQ=255) tAATCCGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGa                                                                              >  1:6295550/1‑100 (MQ=255)  aaTCCGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGaa                                                                             >  1:875263/1‑100 (MQ=255)    gccGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAAcg                                                                           >  1:617311/2‑100 (MQ=255)     ccGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAAcgt                                                                          >  1:3567157/1‑100 (MQ=255)     ccGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAAcgt                                                                          >  1:2970366/1‑100 (MQ=255)     ccGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAAcgt                                                                          >  1:3366384/1‑100 (MQ=255)        tAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTGAACGTGAAcgtcgt                                                                       >  1:4980179/1‑100 (MQ=255)        aaaGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAAcgtcgt                                                                       >  1:1235683/2‑100 (MQ=255)               ttAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTg                                                                <  1:3692496/100‑1 (MQ=255)               ttAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTg                                                                >  1:3539857/1‑100 (MQ=255)                     gagaAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAg                                                          >  1:1218264/1‑100 (MQ=255)                            cAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATCGAGGCAGAACGTGa                                                   >  1:6111637/1‑100 (MQ=255)                            cAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCATGAGATAGCTAAACATACTTCTAAACGTGAACGTCGTGCGATTGAGGCAGAACGTGa                                                   >  1:1711797/1‑100 (MQ=255)                             tctGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATGGAGGCAGATCggaag                                                  >  1:2492062/3‑96 (MQ=255)                             aaTGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGCGGCAGATCggaag                                                  >  1:1891220/1‑96 (MQ=255)                                    aaCAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACtgatg                                           >  1:5736491/1‑100 (MQ=255)                                    aaCAAAGAAGTGAAGCGTTGGGAAGACAAAATGCCTGAGTTAGCTACACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACtgatg                                           >  1:6539986/1‑100 (MQ=255)                                             ttGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGaaaa                                  >  1:4154510/2‑100 (MQ=255)                                             gTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACCTACTTCTAAACGTGAACGCCGTGCTATTGCGGCAGAACGTGATACTGATGAATTGaaaa                                  >  1:6298797/1‑100 (MQ=255)                                               gAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGaaaaaa                                >  1:5251242/1‑100 (MQ=255)                                               ctaGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGaaaaaa                                >  1:1346800/3‑100 (MQ=255)                                                 aGCGTTGGGAAGACAAAGTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCCATTGAGGCAAAACGTGATACTGATGAATTGAAAAAAGc                              <  1:6507777/100‑1 (MQ=255)                                                    cttGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGAAAAAAGCAGa                           >  1:3988227/2‑100 (MQ=255)                                                     tggggAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGAAAAAAGCAGaa                          >  1:4161994/3‑100 (MQ=255)                                                      tGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGCTGAATTGAAAAAAGCAGAat                         >  1:1862105/1‑100 (MQ=255)                                                      tGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGAAAAAAGCAGaaa                         >  1:400444/1‑99 (MQ=255)                                                       gggAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGAAAAAAGCAGAata                        >  1:896422/1‑100 (MQ=255)                                                          gggACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGCGAACGTCGTGCTATTGAGGCAGAACGCGATACTGATGAATTGTAAAAAGCAGAatagat                     >  1:6612442/3‑100 (MQ=255)                                                          acgACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGAAGAAAGCAGAatatat                     >  1:4931708/3‑100 (MQ=255)                                                          aaGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGAACAAAGCAGAatatat                     >  1:4962088/1‑100 (MQ=255)                                                             aCAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGAAAAAAGCAGatcggaagag                  >  1:2937621/1‑91 (MQ=255)                                                                   tGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGAAAAAAGCAGAATATATGATTCAACa            >  1:4709411/1‑100 (MQ=255)                                                                   tGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGAAAAAAGCAGAATATATGATTCAACa            >  1:1193798/1‑100 (MQ=255)                                                                   tGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGAAAAAAGCAGAATATATGATTCAACa            >  1:1589624/1‑100 (MQ=255)                                                                           gtAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGAAAAAAGCAGAATATATGATTCAACATATTGGtg    >  1:5268322/2‑100 (MQ=255)                                                                             aGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGAAAAAAGCAGAATATATGATTCAACATATTGGtgat  >  1:5330294/1‑100 (MQ=255)                                                                                 |                                                                                                TAATCCGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTGAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGAAAAAAGCAGAATATATGATTCAACATATTGGTGAT  >  NC_007793/853231‑853406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑