Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	827,290	T→A	H451Q (CAT→CAA)	SAUSA300_RS04005 →	ABC‑ATPase UvrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	827,290	0	T	A	100.0%	60.9 / NA	24	H451Q (CAT→CAA)	SAUSA300_RS04005	ABC‑ATPase UvrA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (24/0);  total (24/0)

TATCTGTTTATGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACATGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAATGTGGGACTTGAATATTTAACGTTAAACAGAGC  >  NC_007793/827193‑827385                                                                                                  |                                                                                                tATCTGTTTATGTAGGTGGTTTAAATATTGGTGAAGTAGACGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGa                                                                                               >  1:3605773/1‑100 (MQ=255)         tATGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCGGAACAAGATcggaaga                                                                                       >  1:2459474/1‑94 (MQ=255)          aTGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGAt                                                                                      >  1:1463192/1‑100 (MQ=255)           tGTCGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGAtt                                                                                     >  1:6618896/1‑100 (MQ=255)           ggTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCGGAACAAGATcggaagagc                                                                                     >  1:3271905/2‑92 (MQ=255)              aGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGca                                                                                  >  1:2345943/1‑100 (MQ=255)              aGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATGATAAAAACATTGTTTTGTCAGAACAAGATCAAGCGATTGca                                                                                  >  1:4649672/1‑100 (MQ=255)                            ctGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTgaa                                                                    >  1:6519832/2‑100 (MQ=255)                             tGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTgaaa                                                                   >  1:5600948/1‑100 (MQ=255)                             tGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTgaaa                                                                   >  1:3697012/1‑100 (MQ=255)                             tGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTgaaa                                                                   >  1:4444628/1‑100 (MQ=255)                                             tatTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGGAATTATTTCCCGAc                                                   >  1:4814960/1‑100 (MQ=255)                                                 ccctCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCAc                                               >  1:5755302/4‑100 (MQ=255)                                                        ccAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTa                                        >  1:3591560/2‑100 (MQ=255)                                                         cAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTaa                                       >  1:489602/1‑100 (MQ=255)                                                           aGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAaat                                     >  1:3877175/1‑100 (MQ=255)                                                                tGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAAtgt                                >  1:2757781/1‑100 (MQ=255)                                                                 gAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAAtgtg                               >  1:40125/1‑100 (MQ=255)                                                                 gAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAAtgtg                               >  1:306480/1‑100 (MQ=255)                                                                                       cccccAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAATGTGGGACTTGAATATTTAACGTTaa         >  1:4655520/5‑100 (MQ=255)                                                                                           cAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAATGTGGGACTTGAATATTTAACGTTAAACag     >  1:5533196/1‑100 (MQ=255)                                                                                           cAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAATGTGGGACTTGAATATTTAACGTTAAACag     >  1:1616049/1‑100 (MQ=255)                                                                                            aGAACAAGATCAAGCGATTGCAAATCTAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAATGTGGGACTTGAATATTTAACGTTAAACaga    >  1:3199579/1‑100 (MQ=255)                                                                                              caCAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAATGTGGGACTTGAATATTTAACGTTAAACagaga  >  1:2287456/2‑99 (MQ=255)                                                                                                  |                                                                                                TATCTGTTTATGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACATGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAATGTGGGACTTGAATATTTAACGTTAAACAGAGC  >  NC_007793/827193‑827385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑