Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	76,436	A→G	P56P (CCA→CCG)	SAUSA300_RS00345 →	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	76,436	0	A	G	100.0%	61.2 / NA	40	P56P (CCA→CCG)	SAUSA300_RS00345	transposase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/2);  total (38/2)

ATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAA  >  NC_007793/76337‑76510                                                                                                    |                                                                           atatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCa                                                                            >  1:3557158/1‑100 (MQ=11)   ctGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCaga                                                                          >  1:4251511/2‑98 (MQ=11)   ctGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCaga                                                                          >  1:4097378/2‑98 (MQ=11)   atGCATTGAGTTATCGTGATATGTCTGAAATATTAATGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                                                          >  1:1644118/1‑100 (MQ=11)   atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCGTTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                                                          >  1:4449289/1‑100 (MQ=11)   atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCaga                                                                          >  1:4197924/1‑98 (MQ=11)   atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                                                          >  1:6148740/1‑100 (MQ=11)   atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                                                          >  1:5412590/1‑100 (MQ=11)   atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                                                          >  1:4291365/1‑100 (MQ=11)   atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                                                          >  1:3952956/1‑100 (MQ=11)   atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                                                          >  1:275002/1‑100 (MQ=11)   atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                                                          >  1:3096310/1‑100 (MQ=11)   atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATACCCCaga                                                                          >  1:1695972/1‑98 (MQ=11)   atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATACACCCCaga                                                                          >  1:1810249/1‑98 (MQ=11)   atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATAAACCCCaga                                                                          >  1:910549/1‑98 (MQ=11)     gCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAttt                                                                        >  1:82188/1‑100 (MQ=11)     gCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAttt                                                                        >  1:4633952/1‑100 (MQ=11)          gAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTatat                                                                   >  1:6098239/1‑100 (MQ=11)                acGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAAtt                                                             >  1:1152524/2‑100 (MQ=11)                 cGTGATATGTCTGAAATATTAAGGGAACGTGGTGTGAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAAtgg                                                            >  1:1604551/1‑98 (MQ=11)                 cGTGATATGCCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAAtca                                                            >  1:3918234/1‑98 (MQ=11)                  gTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTg                                                           >  1:2377511/1‑100 (MQ=11)                    gATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCaaagatcgg                                                         >  1:6565436/1‑94 (MQ=11)                    gATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGga                                                         >  1:3786212/1‑100 (MQ=11)                    gATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGga                                                         >  1:5457801/1‑100 (MQ=11)                    gATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAACTTGga                                                         <  1:4634781/100‑1 (MQ=11)                    gATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGATCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGga                                                         >  1:2505914/1‑100 (MQ=11)                     ggatgtCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGgaa                                                        >  1:1547136/6‑100 (MQ=11)                     ctatgtCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGgaa                                                        >  1:3219394/2‑100 (MQ=11)                     atatgtCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAAATTggga                                                        <  1:5580656/100‑3 (MQ=11)                       gctccTGTAATATTAAGGGAACGTGGGGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGgaaga                                                      >  1:4680563/5‑100 (MQ=11)                       atgtCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGgaaga                                                      >  1:368640/4‑100 (MQ=11)                         accTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGaaa                                                    >  1:5347265/3‑100 (MQ=11)                          gcTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGaaaa                                                   >  1:5786308/2‑100 (MQ=11)                           cTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGaaaaa                                                  >  1:5074180/1‑100 (MQ=11)                             gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGGGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc                                                >  1:1913774/1‑100 (MQ=255)                             gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGgaagatcggaa                                                >  1:300685/1‑94 (MQ=11)                             gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc                                                >  1:550132/1‑100 (MQ=11)                             gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc                                                >  1:6181985/1‑100 (MQ=11)                             gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc                                                >  1:5386227/1‑100 (MQ=11)                             gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc                                                >  1:2758098/1‑100 (MQ=11)                             gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTCGgaagatcggaa                                                >  1:5549552/1‑94 (MQ=11)                             gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTCGgaagatcggaa                                                >  1:2058973/1‑94 (MQ=11)                             gAAATATCAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc                                                >  1:5809791/1‑100 (MQ=11)                                  atTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGCATaaa                                           >  1:6106909/1‑100 (MQ=11)                                       gggAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagc                                      >  1:1724395/1‑97 (MQ=1)                                                                           agcGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTTATTACAAATGGCGTATTGATGAGACGTACATCaaa  >  1:2568607/3‑100 (MQ=11)                                                                                                    |                                                                           ATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAA  >  NC_007793/76337‑76510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑