Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	780,361	A→T	I334F (ATT→TTT)	SAUSA300_RS03785 →	heme ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	780,361	0	A	T	100.0%	165.5 / NA	57	I334F (ATT→TTT)	SAUSA300_RS03785	heme ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (54/3);  total (54/3)

AGACGATAAAGGTGAATTGAATCTTGCTTATTCAAGATTAGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTAATTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAAACAACACTCTTAAATATTT  >  NC_007793/780264‑780451                                                                                                  |                                                                                           tgACGATAAAGGTGAATTGAATCTTGCTTATTCAAGATTAGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTAttt                                                                                          >  1:3347056/2‑100 (MQ=255) aGACGATAAAGGTGAATTGAATCTTGCTTATTCAAGATTAGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTAttt                                                                                          >  1:872395/1‑100 (MQ=255) aGACGATAAAGGTGAATTGAATCTTGCTTATTCAAGACTAGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTAttt                                                                                          >  1:2531737/1‑100 (MQ=255)   aCGATAAAGGTGAATTGAATCTTGCTTATTCAAGATTAGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGa                                                                                        >  1:547770/1‑100 (MQ=255)        aaaGGTGAATTGAATCTTGCTTATTCAAGATTAGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATc                                                                                   >  1:176386/1‑100 (MQ=255)                         tGCTTATTCAAGATTAGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATCCaa                                                                  >  1:5568087/1‑100 (MQ=255)                             tATTCAAGATTAGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAg                                                              >  1:3392115/1‑100 (MQ=255)                                tCAAGATTAGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTg                                                           <  1:3890391/100‑1 (MQ=255)                                tCAAGATTAGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTg                                                           >  1:827656/1‑100 (MQ=255)                                tCAAGATTAGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTg                                                           <  1:5987869/100‑1 (MQ=255)                                 cAAGATTAGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGa                                                          >  1:4663263/1‑100 (MQ=255)                                     aTTAGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGCGAgatc                                                      >  1:1564370/1‑97 (MQ=255)                                       tAGGTAAGCAAGTGTTCGAATTAGAAGACTTATCACAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGCGAGCGTAt                                                    >  1:5735839/1‑100 (MQ=255)                                        gggTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTAtt                                                   >  1:4042266/2‑100 (MQ=255)                                        aGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAgatcgga                                                   >  1:682350/1‑94 (MQ=255)                                        aGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAgatcgga                                                   >  1:5991153/1‑94 (MQ=255)                                        aGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTAtt                                                   >  1:5732193/1‑100 (MQ=255)                                        aGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTAtt                                                   >  1:5544902/1‑100 (MQ=255)                                        aGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTAtt                                                   >  1:3050022/1‑100 (MQ=255)                                        aGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTAtt                                                   >  1:4637875/1‑100 (MQ=255)                                        aGGTAAGCAAGTATTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTAtt                                                   >  1:2479764/1‑100 (MQ=255)                                           tAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGTTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGgt                                                >  1:543020/1‑100 (MQ=255)                                           tAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGgt                                                >  1:1418044/1‑100 (MQ=255)                                           tAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACACCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGgt                                                >  1:547634/1‑100 (MQ=255)                                            aaGCAAGTGTTCGAATTAGAAGACTTATCATAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGCGAGCGTATTGgtg                                               >  1:3357632/1‑100 (MQ=255)                                            aaGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATATAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGgtg                                               >  1:5095937/1‑100 (MQ=255)                                            aaGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAACTTATTCAAAAAGGTGAgatcggaagag                                               >  1:5512176/1‑90 (MQ=255)                                            aaGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGgtg                                               >  1:3597599/1‑100 (MQ=255)                                            aaGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGgtg                                               >  1:167870/1‑100 (MQ=255)                                            aaGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGgtg                                               >  1:386695/1‑100 (MQ=255)                                            aaGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGgtg                                               >  1:6509139/1‑100 (MQ=255)                                            aaGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGgtg                                               >  1:6386510/1‑100 (MQ=255)                                            aaGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGgtg                                               >  1:6271580/1‑100 (MQ=255)                                            aaGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGAGATCggaagagca                                               >  1:634308/1‑92 (MQ=255)                                             aGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGgtgt                                              >  1:1904495/1‑100 (MQ=255)                                              gCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGgtgta                                             <  1:5582443/100‑2 (MQ=255)                                              gCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGtgtt                                             >  1:670728/1‑100 (MQ=255)                                                  tgtgtgctaTTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGttgttg                                         >  1:5403458/9‑100 (MQ=255)                                                    attCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTggg                                       >  1:2608035/2‑100 (MQ=255)                                                             agaagaCTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGa                              >  1:3722276/1‑100 (MQ=255)                                                               aagaCTTATCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGc                            >  1:2503276/1‑100 (MQ=255)                                                                   actgtgcaaGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGt                        >  1:3517643/8‑100 (MQ=255)                                                                      aTCAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTaaa                     >  1:3212059/1‑100 (MQ=255)                                                                        ccctggCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAaac                   >  1:759392/5‑100 (MQ=255)                                                                        cAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAaac                   >  1:3773539/1‑100 (MQ=255)                                                                        cAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAaac                   >  1:1716306/1‑100 (MQ=255)                                                                        cAAAGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAaac                   >  1:1713244/1‑100 (MQ=255)                                                                         taaGGCTATTAATGATAAAGTATTATTTGAACATCTGCCGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAaaca                  >  1:2895408/2‑100 (MQ=255)                                                                         caaGGCTATTAATGTTAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTAGGCCAAATGGAGCTGGTAAaaca                  >  1:2990655/2‑100 (MQ=255)                                                                         aaaGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAaaca                  >  1:305663/1‑100 (MQ=255)                                                                          gaGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAaacaa                 >  1:2988099/2‑100 (MQ=255)                                                                          aaGGCTATTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAaacaa                 >  1:322212/1‑100 (MQ=255)                                                                                aTTAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAAACAACActct           >  1:1736490/1‑100 (MQ=255)                                                                                 ttAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGCGTTGTTGGGCCAAATGGAGCTGGTAAAACAACACTCtt          >  1:2080878/1‑100 (MQ=255)                                                                                  tAATGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAAACAACACTCTTa         >  1:3589951/1‑100 (MQ=255)                                                                                   aaTGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAAACAACACTCTTaa        >  1:2901315/1‑100 (MQ=255)                                                                                   aaTGATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAAACAACACTCTTaa        >  1:5368235/1‑100 (MQ=255)                                                                                      gATAAAGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAAACAACACTCTTAAata     >  1:5128349/1‑100 (MQ=255)                                                                                         aaaGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAAACAACACTCTTAAATAttt  >  1:3931977/1‑100 (MQ=255)                                                                                         aaaGTATTATTTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAAACAACACTCTTAAATAttt  >  1:5137600/1‑100 (MQ=255)                                                                                                  |                                                                                           AGACGATAAAGGTGAATTGAATCTTGCTTATTCAAGATTAGGTAAGCAAGTGTTCGAATTAGAAGACTTATCAAAGGCTATTAATGATAAAGTATTAATTGAACATCTGACGGAAATTATTCAAAAAGGTGAGCGTATTGGTGTTGTTGGGCCAAATGGAGCTGGTAAAACAACACTCTTAAATATTT  >  NC_007793/780264‑780451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑