Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	759,621	C→G	A234G (GCA→GGA)	SAUSA300_RS03675 →	PTS fructose transporter subunit IIC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	759,621	0	C	G	100.0%	52.6 / NA	21	A234G (GCA→GGA)	SAUSA300_RS03675	PTS fructose transporter subunit IIC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (21/0);  total (21/0)

AAAGCAAGCGGATAAAATGGGTATTAAAATTAAAGTAGAAACGAATGGTTCAAGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGCAACAGGTATCATTGTTGCTGCTGATGTTCATGTTGAGACGGATCGCTTCGATGGTAAAAATGTCGTAGAAGTACCAGTAGCAGATGGTATTAAAC  >  NC_007793/759526‑759716                                                                                                |                                                                                                aaaGCAAGCGGATAAAATGGGTATTAAAATTAAAGTAGAAACGAATGGTTCAAGCGGCATTAAAAACCATTTAATTGAACAAGATATTGAAAATGGAACa                                                                                             >  1:1661066/1‑100 (MQ=255)   tgcaagcGGATAAAATGGGTATTAAAATTAAAGTAGAAACGAATGGTTCAAGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGGAACAgg                                                                                           >  1:962184/2‑100 (MQ=255)    gcaagcGGATAAAATGGGTATTAAAATTAAAGTAGAAACGAATGGTTCAAGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGGAACAGGt                                                                                          >  1:1454122/1‑100 (MQ=255)    ctaagcGGATAAAATGGGTATTAAAATTAAAGTAGAAACGAATGGTTCAAGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGGAACAGGt                                                                                          >  1:506634/3‑100 (MQ=255)      ttgcGGATAAAATGGGTATTAAAATTAAAGTAGAAACGAATGGTTCAAGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGGAACAGGTAt                                                                                        >  1:89964/3‑100 (MQ=255)              aaaaTGGGTATTAAAATTAAAGTAGAAACGAATGGTTCAAGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGGAACAGGTATCAttgttg                                                                                >  1:2853740/1‑100 (MQ=255)              aaaaTGGGTATTAAAATTAAAGTAGAAACGAATGGTTCAAGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGGAACAGGTATCAttgttg                                                                                >  1:6272029/1‑100 (MQ=255)              aaaaTGGGTATTAAAATTAAAGTAGAAACGAATGGTTCAAGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGGAACAGGTATCAttgttg                                                                                >  1:4682591/1‑100 (MQ=255)              aaaaTGGGTATTAAAATTAAAGTAGAAACGAATGGTTCAAGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGGAACAGGTATCAttgttg                                                                                >  1:6085311/1‑100 (MQ=255)              aaaaTGGGTATTAAAATTAAAGTAGAAACGAATGGCTCAAGCGGTATTAAAAACCATTTAACTGAACAAGATATTGAAAATGGAACAGGTATCAttgctg                                                                                >  1:4961787/1‑100 (MQ=255)                caTGGGTATTAAAATTAAAGTAGAAACGAATGGTTCAAGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGGAACAGGTATCATTGTtgct                                                                              >  1:2469372/2‑100 (MQ=255)                 ttGGGTATTAAAATTAAAGTAGAAACGAATGGTTCAAGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGGAACAGGTATCATTGTtgctg                                                                             >  1:6290053/2‑100 (MQ=255)                               taaaGTAGAAACGAATGGTTCAAGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGGAACAGGTATCATTGTTGCTGCTGATGTTCATGtt                                                               >  1:1543913/1‑100 (MQ=255)                                              tGGTTCAAGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGGAACAGGTATCATTGTTGCTGCTGATGTTCATGTTGAGACGGATCGCTTc                                                >  1:2773819/1‑100 (MQ=255)                                               ggTTCAAGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGGAACAGGTATCATTGTTGCTGCTGATGTTCATGTTGGGACGGATCGCTTCg                                               >  1:4869983/1‑100 (MQ=255)                                                   cAAGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGGAACAGGTATCATTGTTGCTGCTGATGTTCATGTTGAGACGGATCGCTTCGATgg                                           >  1:4571453/1‑100 (MQ=255)                                                    caGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGGAACAGGTATCATTGTTGCTGCTGATGTTCATGTTGAGACGGATCGCTTCGATGGt                                          >  1:5947790/2‑100 (MQ=255)                                                                       tctccTGAACAAGATATTGAAAATGGAACAGGTATCATTGTTGCTGCTGATGTTCATGTTGAGACGGATCGCTTCGATGGTAAAAATGTCGTAGAAGTAc                       >  1:3473637/5‑100 (MQ=255)                                                                       gtAACTGAACAAGATATTGAAAATGGAACAGGTATCATTGTTGCTGCTGATGTTCATGTTGAGACGGATCGCTTCGATGGTAAAAATGTCGTAGAAGTAc                       >  1:2623079/2‑100 (MQ=255)                                                                             gAACAAGATATTGAAAATGGAACAGGTATCATTGTTGCTGCTGATGTTCATGTTGAGACGGATCGCTTCGATGGTAAAAATGTCGTAGAAGTACCAGTAg                 >  1:6602399/1‑100 (MQ=255)                                                                                            aaTGGAACAGGTATCATTGTTGCTGCTGATGTTCATGTTGAGACGGATCGCTTCGATGGTAAAAATGTCGTAGAAGTACCAGTAGCAGATGGTATTAAAc  >  1:6158043/1‑100 (MQ=255)                                                                                                |                                                                                                AAAGCAAGCGGATAAAATGGGTATTAAAATTAAAGTAGAAACGAATGGTTCAAGCGGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGCAACAGGTATCATTGTTGCTGCTGATGTTCATGTTGAGACGGATCGCTTCGATGGTAAAAATGTCGTAGAAGTACCAGTAGCAGATGGTATTAAAC  >  NC_007793/759526‑759716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑