Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	757,384	C→T	L50L (CTG→TTG)	SAUSA300_RS03665 →	DeoR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	757,384	0	C	T	100.0%	22.2 / NA	10	L50L (CTG→TTG)	SAUSA300_RS03665	DeoR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/2);  total (8/2)

ATCGAACTGGTTGCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAACTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTATGGTTGAGGCGAATTTAACTGAAAAATTAGCAACGAATCTT  >  NC_007793/757319‑757467                                                                  |                                                                                    tccGATCTGGTTGCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGa                                                   <  1:3194002/98‑1 (MQ=255)   cGAACTGGTTGCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGaaa                                                 >  1:4348183/1‑100 (MQ=255)   cGAACTGGTTGCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGaaa                                                 >  1:5680588/1‑100 (MQ=255)         ggTGGCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTa                                           >  1:1223683/1‑100 (MQ=255)             gCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGaaagatcggaaga                                       >  1:5386044/1‑90 (MQ=255)               aGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTATGGTTg                                     <  1:4534040/100‑1 (MQ=255)                                             aaaCTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTATGGTTGAGGCGAATTTAACTGAAAAATTAGCAACGa       >  1:4746866/1‑100 (MQ=255)                                                  acaacaATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTATGGTTGAGGCGAATTTAACTGAAAAATTAGCAACGAATCtt  >  1:1194366/1‑100 (MQ=255)                                                  acaacaATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTATGGTTGAGGCGAATTTAACTGAAAAATTAGCAACGAATCtt  >  1:2275327/1‑100 (MQ=255)                                                  acaacaATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTATGGTTGAGGCGAATTTAACTGAAAAATTAGCAACGAATCtt  >  1:5291026/1‑100 (MQ=255)                                                                  |                                                                                    ATCGAACTGGTTGCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAACTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTATGGTTGAGGCGAATTTAACTGAAAAATTAGCAACGAATCTT  >  NC_007793/757319‑757467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑