Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 76,418 G→A W50* (TGG→TGA)  SAUSA300_RS00345 → transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00779376,4180GA98.2% 169.7 / ‑5.1 108W50* (TGG→TGA) SAUSA300_RS00345transposase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (104/2);  total (106/2)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 108 total aligned reads displayed.

TTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAA  >  NC_007793/76321‑76510
                                                                                                 |                                                                                            
ttGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGt                                                                                            >  1:4535902/1‑100 (MQ=11)
ttGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGt                                                                                            >  1:4027755/1‑100 (MQ=11)
ctGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGt                                                                                            >  1:6611273/2‑100 (MQ=11)
 tGGCTGCTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                                                           >  1:6459776/1‑100 (MQ=11)
 tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                                                           >  1:1382480/1‑100 (MQ=11)
 tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                                                           >  1:1442476/1‑100 (MQ=11)
 tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                                                           >  1:1464514/1‑100 (MQ=11)
 tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                                                           >  1:896508/1‑100 (MQ=11)
 tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                                                           >  1:2415610/1‑100 (MQ=11)
 tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                                                           >  1:4030132/1‑100 (MQ=11)
 tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                                                           >  1:3539589/1‑100 (MQ=11)
 tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                                                           >  1:2198139/1‑100 (MQ=11)
 tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                                                           >  1:69282/1‑100 (MQ=11)
 tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGATCTAGCGTTGAGtt                                                                                           >  1:4837097/1‑100 (MQ=11)
  ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTc                                                                                          >  1:2845010/1‑100 (MQ=11)
  ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTc                                                                                          >  1:6079586/1‑100 (MQ=11)
  ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTc                                                                                          >  1:3670492/1‑100 (MQ=11)
  ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTc                                                                                          >  1:5918183/1‑100 (MQ=11)
  ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTc                                                                                          >  1:1100658/1‑100 (MQ=11)
  ggCTACTATCTAAGATATGCATTGAGATATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTc                                                                                          >  1:4734758/1‑100 (MQ=11)
      actaTCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGa                                                                                      >  1:2103384/1‑100 (MQ=11)
      actaTCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGa                                                                                      >  1:6493009/1‑100 (MQ=11)
      actaTCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGa                                                                                      >  1:3174407/1‑100 (MQ=11)
      actaTCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGa                                                                                      >  1:27442/1‑100 (MQ=11)
         aTCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAata                                                                                   >  1:1860182/1‑100 (MQ=11)
         aTCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAata                                                                                   >  1:2769448/1‑100 (MQ=11)
            aaaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGGGTTCAAGAATATGc                                                                                >  1:4968976/2‑100 (MQ=255)
             aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAGTATGcc                                                                               >  1:2489148/1‑100 (MQ=11)
             aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                                                               >  1:4339015/1‑100 (MQ=11)
             aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                                                               >  1:3057598/1‑100 (MQ=11)
             aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                                                               >  1:2490778/1‑100 (MQ=11)
             aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                                                               >  1:2887203/1‑100 (MQ=11)
             aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                                                               >  1:2786096/1‑100 (MQ=11)
             aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                                                               >  1:5557220/1‑100 (MQ=11)
             aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                                                               >  1:6462360/1‑100 (MQ=11)
             aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                                                               >  1:2288241/1‑100 (MQ=11)
             aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                                                               >  1:5450606/1‑100 (MQ=11)
             aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                                                               >  1:1197796/1‑100 (MQ=11)
             aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGGTCAAGAATATGcc                                                                               >  1:6473925/1‑100 (MQ=11)
             aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAATTCAAGAATACGcc                                                                               >  1:4946783/1‑100 (MQ=11)
              tgATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGccc                                                                              >  1:4159543/2‑100 (MQ=11)
                taatGCATTGAGTTATCGTGATATGTCTGAAAAATTAAGGGAACGTGGTGTAAACGTTCATCATGTAACGGTCAAGCGTTGAGTTCAAGAATATGccccg                                                                            >  1:855767/3‑99 (MQ=11)
                atatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGccccg                                                                            >  1:434885/1‑99 (MQ=11)
                atatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGccccg                                                                            >  1:5074940/1‑99 (MQ=11)
                atatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGccccg                                                                            >  1:5784564/1‑99 (MQ=11)
                atatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCa                                                                            >  1:3557158/1‑100 (MQ=11)
                atacGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATGTGccccg                                                                            >  1:3080560/1‑99 (MQ=11)
                 tatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCGAGAATATGccccga                                                                           >  1:4821803/1‑98 (MQ=11)
                 tatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGccccga                                                                           >  1:5819722/1‑98 (MQ=11)
                 tatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGccccga                                                                           >  1:3809121/1‑98 (MQ=11)
                 gatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGccccga                                                                           >  1:80668/2‑98 (MQ=11)
                 catGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGccccga                                                                           >  1:3412529/2‑98 (MQ=11)
                 aatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCGAGAATATGccccga                                                                           >  1:4914337/2‑98 (MQ=11)
                  ctGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCaga                                                                          >  1:4097378/2‑98 (MQ=11)
                  ctGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCaga                                                                          >  1:4251511/2‑98 (MQ=11)
                  atGCATTGAGTTATCGTGATATGTCTGAAATATTAATGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                                                          >  1:1644118/1‑100 (MQ=11)
                  atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCGTTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                                                          >  1:4449289/1‑100 (MQ=11)
                  atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcccctag                                                                          >  1:3440041/1‑97 (MQ=11)
                  atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcccccag                                                                          >  1:1565396/1‑97 (MQ=11)
                  atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCaga                                                                          >  1:4197924/1‑98 (MQ=11)
                  atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                                                          >  1:3096310/1‑100 (MQ=11)
                  atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                                                          >  1:6148740/1‑100 (MQ=11)
                  atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                                                          >  1:275002/1‑100 (MQ=11)
                  atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                                                          >  1:5412590/1‑100 (MQ=11)
                  atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                                                          >  1:4291365/1‑100 (MQ=11)
                  atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                                                          >  1:3952956/1‑100 (MQ=11)
                  atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATACCCCaga                                                                          >  1:1695972/1‑98 (MQ=11)
                  atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATACACCCCaga                                                                          >  1:1810249/1‑98 (MQ=11)
                  atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATAAACCCCaga                                                                          >  1:910549/1‑98 (MQ=11)
                    gCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAttt                                                                        >  1:82188/1‑100 (MQ=11)
                    gCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAttt                                                                        >  1:4633952/1‑100 (MQ=11)
                         gAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTatat                                                                   >  1:6098239/1‑100 (MQ=11)
                               acGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAAtt                                                             >  1:1152524/2‑100 (MQ=11)
                                cGTGATATGTCTGAAATATTAAGGGAACGTGGTGTGAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAAtgg                                                            >  1:1604551/1‑98 (MQ=11)
                                cGTGATATGCCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAAtca                                                            >  1:3918234/1‑98 (MQ=11)
                                 gTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTg                                                           >  1:2377511/1‑100 (MQ=11)
                                   gATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCaaagatcgg                                                         >  1:6565436/1‑94 (MQ=11)
                                   gATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGga                                                         >  1:5457801/1‑100 (MQ=11)
                                   gATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGga                                                         >  1:3786212/1‑100 (MQ=11)
                                   gATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAACTTGga                                                         <  1:4634781/100‑1 (MQ=11)
                                   gATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGATCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGga                                                         >  1:2505914/1‑100 (MQ=11)
                                    ggatgtCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGgaa                                                        >  1:1547136/6‑100 (MQ=11)
                                    ctatgtCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGgaa                                                        >  1:3219394/2‑100 (MQ=11)
                                    atatgtCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAAATTggga                                                        <  1:5580656/100‑3 (MQ=11)
                                      gctccTGTAATATTAAGGGAACGTGGGGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGgaaga                                                      >  1:4680563/5‑100 (MQ=11)
                                      atgtCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGgaaga                                                      >  1:368640/4‑100 (MQ=11)
                                        accTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGaaa                                                    >  1:5347265/3‑100 (MQ=11)
                                         gcTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGaaaa                                                   >  1:5786308/2‑100 (MQ=11)
                                          cTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGaaaaa                                                  >  1:5074180/1‑100 (MQ=11)
                                            gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGGGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc                                                >  1:1913774/1‑100 (MQ=255)
                                            gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGgaagatcggaa                                                >  1:300685/1‑94 (MQ=11)
                                            gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc                                                >  1:2758098/1‑100 (MQ=11)
                                            gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc                                                >  1:6181985/1‑100 (MQ=11)
                                            gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc                                                >  1:5386227/1‑100 (MQ=11)
                                            gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTCGgaagatcggaa                                                >  1:2058973/1‑94 (MQ=11)
                                            gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTCGgaagatcggaa                                                >  1:5549552/1‑94 (MQ=11)
                                            gAAATATCAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc                                                >  1:5809791/1‑100 (MQ=11)
                                                 atTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGCATaaa                                           >  1:6106909/1‑100 (MQ=11)
                                                      gggAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagc                                      >  1:1724395/1‑97 (MQ=1)
                                                                                          agcGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTTATTACAAATGGCGTATTGATGAGACGTACATCaaa  >  1:2568607/3‑100 (MQ=11)
                                                                                                 |                                                                                            
TTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAA  >  NC_007793/76321‑76510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑