Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	735,887	G→A	M215I (ATG→ATA)	SAUSA300_RS03540 →	sugar MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	735,887	0	G	A	100.0%	109.2 / NA	41	M215I (ATG→ATA)	SAUSA300_RS03540	sugar MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/0);  total (41/0)

ACTTTAGTACTTCAAGTGTTTTTCTATAAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATGTTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTATTTGTT  >  NC_007793/735795‑735980                                                                                             |                                                                                              aCTTTAGTACTTCAAGTGTTTTTCTATAAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAg                                                                                        >  1:347334/1‑100 (MQ=255)    ttAGTACTTCAAGTGTTTTTCTATAAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACa                                                                                     >  1:6098517/1‑100 (MQ=255)         aCTTCAAGTGTTTTTCTATAAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAGAATTGCTCCTAATATATTTAAAGACAAAACg                                                                                >  1:5340873/1‑100 (MQ=255)            tCAAGTGTTTTTCTATAAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCtt                                                                             >  1:124298/1‑100 (MQ=255)                        cTATAAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTG‑AAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTa                                                                >  1:4899662/1‑100 (MQ=255)                          ataAGGCTTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTAt                                                               >  1:4073877/1‑100 (MQ=255)                          ataAGGATTTAAACATTAAACACCCTATTAGTACGTAAAAACATGGTGAAAAAATTGCTCCTAATATATTTAAAGACAGAACGCTTTTATTCCCATTTAt                                                               >  1:340918/1‑100 (MQ=255)                          ataAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGCAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTAt                                                               >  1:6166340/1‑100 (MQ=255)                          ataAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTAt                                                               >  1:1505328/1‑100 (MQ=255)                          ataAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTAt                                                               >  1:2704131/1‑100 (MQ=255)                          ataAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTAt                                                               >  1:5219594/1‑100 (MQ=255)                          ataAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTAt                                                               >  1:4942161/1‑100 (MQ=255)                          ataAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTAt                                                               >  1:6419055/1‑100 (MQ=255)                          ataAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTAt                                                               >  1:1288157/1‑100 (MQ=255)                           taAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTAtt                                                              >  1:6399086/1‑100 (MQ=255)                                    aaacattaaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCatttatt                                                     >  1:4983173/1‑100 (MQ=255)                                    aaacattaaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCatttatt                                                     >  1:4710831/1‑100 (MQ=255)                                          taaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTttatta                                               >  1:5229497/1‑100 (MQ=255)                                          taaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTttatta                                               >  1:4307701/1‑100 (MQ=255)                                           aaacaCCCTATTAGTACGCAACAACCTGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTac                                              >  1:2589000/1‑100 (MQ=255)                                           aaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTac                                              >  1:3302733/1‑100 (MQ=255)                                           aaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTac                                              >  1:3522243/1‑100 (MQ=255)                                            atcacCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTaca                                             >  1:129208/3‑100 (MQ=255)                                            aacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCCTTTATTTTATTaca                                             >  1:4865334/1‑100 (MQ=255)                                            aacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTaca                                             >  1:1920414/1‑100 (MQ=255)                                            aacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTaca                                             >  1:4532525/1‑100 (MQ=255)                                             acacCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTacac                                            >  1:1148013/1‑100 (MQ=255)                                             acacCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTacac                                            >  1:1596469/1‑100 (MQ=255)                                              cacCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTAcaca                                           >  1:6412847/1‑100 (MQ=255)                                                      tAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACaa                                   >  1:941398/1‑100 (MQ=255)                                                      ctgTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACaa                                   >  1:5859115/3‑100 (MQ=255)                                                          aCGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGa                               >  1:4146947/1‑100 (MQ=255)                                                          aCGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACACTGGACagatcg                               >  1:4523713/1‑95 (MQ=255)                                                                                 tGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTa        >  1:3934825/1‑100 (MQ=255)                                                                                 tGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTa        >  1:1684610/1‑100 (MQ=255)                                                                                 tGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTa        >  1:1372397/1‑100 (MQ=255)                                                                                     ccTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTATTTg    >  1:3386491/1‑100 (MQ=255)                                                                                     ccTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTATTTg    >  1:45038/1‑100 (MQ=255)                                                                                     ccTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTATTTg    >  1:4359930/1‑100 (MQ=255)                                                                                     ccTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTATTTg    >  1:4357363/1‑100 (MQ=255)                                                                                       tAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTATTTGtt  >  1:3065838/1‑100 (MQ=255)                                                                                             |                                                                                              ACTTTAGTACTTCAAGTGTTTTTCTATAAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATGTTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTATTTGTT  >  NC_007793/735795‑735980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑