Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	730,141	A→G	Q394R (CAA→CGA)	SAUSA300_RS03500 →	AraC family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	730,141	0	A	G	100.0%	50.8 / NA	21	Q394R (CAA→CGA)	SAUSA300_RS03500	AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/2);  total (19/2)

ATTGAAAGTGATCTAAAGATAGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACAAATATCTCCAAACGAATTAAGTAATCATAATAATTACGAAGTGCATTTTGTTTTTGATTTATCATTGATGGAA  >  NC_007793/730053‑730214                                                                                         |                                                                          tggggaaGTGATCTAAAGATAGCATTTAATATAAATAATATCGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCaa                                                                >  1:6546324/6‑100 (MQ=255)             cTAAAGATAGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTa                                                    >  1:1119990/1‑100 (MQ=255)              caaaGATAGCATTTCATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTaa                                                   >  1:1748407/2‑100 (MQ=255)               aaaGATAGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAGACGAATTAAGTAAt                                                  >  1:4929990/1‑100 (MQ=255)               aaaGATAGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCACACGAATTAAGTAAt                                                  >  1:4907030/1‑100 (MQ=255)               aaaGATAGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTaaa                                                  >  1:22609/1‑99 (MQ=255)               aaaGATAGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTAAt                                                  >  1:1512595/1‑100 (MQ=255)               aaaGATAGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTAAt                                                  >  1:2120143/1‑100 (MQ=255)               aaaGATAGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTAAt                                                  >  1:6404441/1‑100 (MQ=255)               aaaGATAGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTAAt                                                  >  1:4767379/1‑100 (MQ=255)               aaaGATAGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTAAt                                                  >  1:5874682/1‑100 (MQ=255)                aaGATAGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTAATc                                                 <  1:2632944/100‑1 (MQ=255)                 agctAGCATTTAATATGAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATACCTCCAAACGAATTAAGTAAGCa                                                >  1:1662438/4‑100 (MQ=255)                 aGATAGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTAATCa                                                >  1:647475/1‑100 (MQ=255)                  cctAGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTAATCat                                               >  1:4617933/3‑100 (MQ=255)                   aTAGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTAATCata                                              >  1:2455034/1‑100 (MQ=255)                     aGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTAATCataat                                            >  1:243424/1‑100 (MQ=255)                     aGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTAATCataat                                            >  1:822693/1‑100 (MQ=255)                     aGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTAACCaaata                                            >  1:5341933/1‑96 (MQ=255)                                    taataTTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTAATCATAATAATTACGAAGTGCaa                             <  1:791633/100‑2 (MQ=255)                                                               agaagCTTTTATGAGTGTTTTCAGACGAATATCTCCAAACGAATTAAGTAATCATAATAATTACGAAGTGCATTTTGTTTTTGATTTATCATTGATGGaa  >  1:2172433/1‑100 (MQ=255)                                                                                         |                                                                          ATTGAAAGTGATCTAAAGATAGCATTTAATATAAATAATATTGAAACAACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACAAATATCTCCAAACGAATTAAGTAATCATAATAATTACGAAGTGCATTTTGTTTTTGATTTATCATTGATGGAA  >  NC_007793/730053‑730214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑