Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	725,905	T→C	intergenic (+31/+557)	SAUSA300_RS03485 → / ← SAUSA300_RS03490	inorganic phosphate transporter/peptidase M23

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	725,905	0	T	C	100.0%	45.2 / NA	19	intergenic (+31/+557)	SAUSA300_RS03485/SAUSA300_RS03490	inorganic phosphate transporter/peptidase M23
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/19);  total (0/19)

TTACATTACCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAGTATCACAAACAT  >  NC_007793/725810‑725914                                                                                                |          ttacattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAATACcacc       <  1:1569656/100‑2 (MQ=255) ttacattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAATACcacc       <  1:6409747/100‑2 (MQ=255) ttacattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAATACcacc       <  1:3024345/100‑2 (MQ=255) ttacattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAATACcacc       <  1:5795212/100‑2 (MQ=255) ttacattacCTATTCCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAATACcacc       <  1:4995756/100‑2 (MQ=255)  tacattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAATACcacct      <  1:2461050/100‑3 (MQ=255)  tacattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAATACcacca      <  1:1620327/100‑3 (MQ=255)  tacattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAATACcacca      <  1:1394836/100‑3 (MQ=255)   acattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAGCTAAACTGAACTTCAATACcaccat     <  1:3674481/100‑4 (MQ=255)   acattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAGCAAAACTGAACTTCAATACcaccat     <  1:846606/100‑4 (MQ=255)   acattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAATACcaccat     <  1:505203/100‑4 (MQ=255)   acattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAATACcaccat     <  1:5495747/100‑4 (MQ=255)   acattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAATACcaccat     <  1:5829560/100‑4 (MQ=255)   acattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAATACcaccat     <  1:2418033/100‑4 (MQ=255)   acattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAATACcaccat     <  1:6496655/100‑4 (MQ=255)   acattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAATACCACcaa     <  1:4439501/100‑1 (MQ=255)   acattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAATACCACcaa     <  1:3061719/100‑1 (MQ=255)    cattacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACAAAACTGAACTTCAATACcaccata    <  1:1780606/100‑5 (MQ=255)      ttacCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAATACcaccaggct  <  1:4501470/100‑7 (MQ=255)                                                                                                |          TTACATTACCTATTTCAGCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAATTGAAAATAAAACTAAACTGAACTTCAGTATCACAAACAT  >  NC_007793/725810‑725914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑