Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 76,412 C→G Y48* (TAC→TAG)  SAUSA300_RS00345 → transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00779376,4120CG99.2% 201.4 / ‑5.6 120Y48* (TAC→TAG) SAUSA300_RS00345transposase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (116/3);  minor base A (1/0);  total (117/3)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 120 total aligned reads displayed.

CCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCT  >  NC_007793/76318‑76474
                                                                                              |                                                              
ccGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTg                                                           >  1:470165/1‑100 (MQ=255)
ccGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTg                                                           <  1:5337079/100‑1 (MQ=255)
 gcatGGCTACTATCTAAGATCTGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTga                                                          >  1:3868220/4‑99 (MQ=11)
 ctgtGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTga                                                          >  1:5986129/4‑99 (MQ=11)
 ccctGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTga                                                          >  1:4539096/4‑99 (MQ=11)
  cttGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTgag                                                         >  1:930581/2‑98 (MQ=11)
  cttGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTgag                                                         >  1:5143440/2‑98 (MQ=11)
  cctGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAACGTTgag                                                         >  1:856048/3‑98 (MQ=11)
   ttGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCCTCATTTAACGGTCTAGCGTTGAGt                                                        >  1:4742911/1‑100 (MQ=11)
   ctGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTgaga                                                        >  1:5076083/2‑97 (MQ=11)
   ctGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGt                                                        >  1:6611273/2‑100 (MQ=11)
    tGGCTGCTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                       >  1:6459776/1‑100 (MQ=11)
    tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTgagat                                                       >  1:1615361/1‑96 (MQ=11)
    tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                       >  1:3539589/1‑100 (MQ=11)
    tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                       >  1:4030132/1‑100 (MQ=11)
    tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                       >  1:268817/1‑100 (MQ=11)
    tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                       >  1:2198139/1‑100 (MQ=11)
    tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                       >  1:1822859/1‑100 (MQ=11)
    tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                       >  1:69282/1‑100 (MQ=11)
    tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                       >  1:1442476/1‑100 (MQ=11)
    tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                       >  1:1382480/1‑100 (MQ=11)
    tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGtt                                                       >  1:896508/1‑100 (MQ=11)
    tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGATCTAGCGTTGAGtt                                                       >  1:4837097/1‑100 (MQ=11)
     ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTgagatc                                                      >  1:692659/1‑95 (MQ=11)
     ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTgagatc                                                      >  1:4281941/1‑95 (MQ=11)
     ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTgagatc                                                      >  1:2077121/1‑95 (MQ=11)
     ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTc                                                      >  1:6079586/1‑100 (MQ=11)
     ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTc                                                      >  1:5918183/1‑100 (MQ=11)
     ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTc                                                      >  1:2845010/1‑100 (MQ=11)
     ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTc                                                      >  1:1100658/1‑100 (MQ=11)
     ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTc                                                      >  1:3670492/1‑100 (MQ=11)
         actaTCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGa                                                  >  1:2103384/1‑100 (MQ=11)
         actaTCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGa                                                  >  1:3174407/1‑100 (MQ=11)
         actaTCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGa                                                  >  1:27442/1‑100 (MQ=11)
            aTCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAata                                               >  1:2769448/1‑100 (MQ=11)
            aTCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAata                                               >  1:1860182/1‑100 (MQ=11)
                aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAGTATGcc                                           >  1:2489148/1‑100 (MQ=11)
                aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                           >  1:2288241/1‑100 (MQ=11)
                aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                           >  1:2786096/1‑100 (MQ=11)
                aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                           >  1:5557220/1‑100 (MQ=11)
                aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                           >  1:1197796/1‑100 (MQ=11)
                aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                           >  1:5450606/1‑100 (MQ=11)
                aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                           >  1:2490778/1‑100 (MQ=11)
                aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                           >  1:4047997/1‑100 (MQ=11)
                aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                           >  1:6353524/1‑100 (MQ=11)
                aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                           >  1:4339015/1‑100 (MQ=11)
                aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                           >  1:2887203/1‑100 (MQ=11)
                aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                           >  1:6462360/1‑100 (MQ=11)
                aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcc                                           >  1:3057598/1‑100 (MQ=11)
                aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGGTCAAGAATATGcc                                           >  1:6473925/1‑100 (MQ=11)
                aaGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAATTCAAGAATACGcc                                           >  1:4946783/1‑100 (MQ=11)
                 tgATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGccc                                          >  1:4159543/2‑100 (MQ=11)
                   taatGCATTGAGTTATCGTGATATGTCTGAAAAATTAAGGGAACGTGGTGTAAACGTTCATCATGTAACGGTCAAGCGTTGAGTTCAAGAATATGccccg                                        >  1:855767/3‑99 (MQ=11)
                   atatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGccccg                                        >  1:434885/1‑99 (MQ=11)
                   atatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGccccg                                        >  1:5074940/1‑99 (MQ=11)
                   atatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGccccg                                        >  1:5784564/1‑99 (MQ=11)
                   atatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCa                                        >  1:3557158/1‑100 (MQ=11)
                   atacGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATGTGccccg                                        >  1:3080560/1‑99 (MQ=11)
                    tatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCGAGAATATGccccga                                       >  1:4821803/1‑98 (MQ=11)
                    tatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGccccga                                       >  1:3809121/1‑98 (MQ=11)
                    gatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGccccga                                       >  1:80668/2‑98 (MQ=11)
                    catGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGccccga                                       >  1:3412529/2‑98 (MQ=11)
                    aatGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCGAGAATATGccccga                                       >  1:4914337/2‑98 (MQ=11)
                     ctGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCaga                                      >  1:4097378/2‑98 (MQ=11)
                     atGCATTGAGTTATCGTGATATGTCTGAAATATTAATGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                      >  1:1644118/1‑100 (MQ=11)
                     atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCGTTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                      >  1:4449289/1‑100 (MQ=11)
                     atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcccctag                                      >  1:3440041/1‑97 (MQ=11)
                     atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGcccccag                                      >  1:1565396/1‑97 (MQ=11)
                     atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCaga                                      >  1:4197924/1‑98 (MQ=11)
                     atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                      >  1:3096310/1‑100 (MQ=11)
                     atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                      >  1:4291365/1‑100 (MQ=11)
                     atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                      >  1:5412590/1‑100 (MQ=11)
                     atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAt                                      >  1:3952956/1‑100 (MQ=11)
                     atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATACACCCCaga                                      >  1:1810249/1‑98 (MQ=11)
                     atGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATAAACCCCaga                                      >  1:910549/1‑98 (MQ=11)
                       gCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGAttt                                    >  1:4633952/1‑100 (MQ=11)
                            gAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTatat                               >  1:6098239/1‑100 (MQ=11)
                                  acGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAAtt                         >  1:1152524/2‑100 (MQ=11)
                                   cGTGATATGTCTGAAATATTAAGGGAACGTGGTGTGAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAAtgg                        >  1:1604551/1‑98 (MQ=11)
                                   cGTGATATGCCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAAtca                        >  1:3918234/1‑98 (MQ=11)
                                    gTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTg                       >  1:2377511/1‑100 (MQ=11)
                                      gATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCaaagatcgg                     >  1:6565436/1‑94 (MQ=11)
                                      gATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGga                     >  1:3786212/1‑100 (MQ=11)
                                      gATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGga                     >  1:5457801/1‑100 (MQ=11)
                                      gATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAACTTGga                     <  1:4634781/100‑1 (MQ=11)
                                      gATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGATCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGga                     >  1:2505914/1‑100 (MQ=11)
                                       ggatgtCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGgaa                    >  1:1547136/6‑100 (MQ=11)
                                       ctatgtCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGgaa                    >  1:3219394/2‑100 (MQ=11)
                                       atatgtCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAAATTggga                    <  1:5580656/100‑3 (MQ=11)
                                         gctccTGTAATATTAAGGGAACGTGGGGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGgaaga                  >  1:4680563/5‑100 (MQ=11)
                                            gcTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGaaaa               >  1:5786308/2‑100 (MQ=11)
                                             cTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGaaaaa              >  1:5074180/1‑100 (MQ=11)
                                               gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGGGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc            >  1:1913774/1‑100 (MQ=255)
                                               gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc            >  1:5386227/1‑100 (MQ=11)
                                               gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc            >  1:6181985/1‑100 (MQ=11)
                                               gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc            >  1:2758098/1‑100 (MQ=11)
                                               gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc            >  1:550132/1‑100 (MQ=11)
                                               gAAATATCAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGc            >  1:5809791/1‑100 (MQ=11)
                                                    atTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGCATaaa       >  1:6106909/1‑100 (MQ=11)
                                                         gggAACGTGGTGTAAACGTTCATCATTTAACGGTCTAGCGTTGAGTTCAAGAATATGCCCCGATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagc  >  1:1724395/1‑97 (MQ=1)
                                                                                              |                                                              
CCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCT  >  NC_007793/76318‑76474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑