Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	721,388	T→C	L174L (TTG→CTG)	SAUSA300_RS03470 →	ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	721,388	0	T	C	100.0%	185.7 / NA	72	L174L (TTG→CTG)	SAUSA300_RS03470	ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (71/1);  total (71/1)

CTCTGAATTGTCTGGTGGACAAAGGCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCATTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATCCTGTTG  >  NC_007793/721291‑721484                                                                                                  |                                                                                                 ctctGAATTGTCTGGTGGACAAAGGCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTg                                                                                                >  1:5638637/1‑100 (MQ=255) ctctGAATTGTCTGGTGGACAAAGGCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTg                                                                                                >  1:3394390/1‑100 (MQ=255)  cctGAATTGTCTGGTGGACAAAGGCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTACATTTGCTGACGAACCAACAGGTGCACTgg                                                                                               >  1:1686377/2‑100 (MQ=255)   ctGAATTGTCTGGTGGACAAAGGCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGa                                                                                              >  1:3240094/1‑100 (MQ=255)     gAATTGTCTGGTGGACAAAGGCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGAtt                                                                                            >  1:1182965/1‑100 (MQ=255)     gAATTGTCTGGTGGACAAAGGCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGAtt                                                                                            >  1:2972504/1‑100 (MQ=255)     gAATTGTCTGGTGGACAAAGGCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGAtt                                                                                            >  1:3928092/1‑100 (MQ=255)     gAATTGTCTGGTGGACAAAGGCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGAGCACTGGAtt                                                                                            >  1:872425/1‑100 (MQ=255)              ggtggACAAAGGCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTa                                                                                   >  1:906776/1‑100 (MQ=255)              ggtggACAAAGGCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTa                                                                                   >  1:6642738/1‑100 (MQ=255)              cgtggACAAAGGCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGCGCACTGGATTCTAAAAGTa                                                                                   >  1:4660784/2‑100 (MQ=255)                tggACAAAGGCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACt                                                                                 >  1:6433462/1‑100 (MQ=255)                  gACAAAGGCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCa                                                                               <  1:2137388/100‑1 (MQ=255)                        tgCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGAttt                                                                         >  1:3433557/2‑100 (MQ=255)                         gCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCGAGAtcgg                                                                        >  1:5608515/1‑97 (MQ=255)                           aaCGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGAtcggaa                                                                      >  1:4160363/1‑95 (MQ=255)                            tcGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATttatta                                                                     >  1:2119294/2‑100 (MQ=255)                                    cAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGAtt                                                             >  1:5075492/1‑100 (MQ=255)                                    cAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGAtt                                                             >  1:1877534/1‑100 (MQ=255)                                     ggCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTa                                                            >  1:6184459/2‑100 (MQ=255)                                       cTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAAc                                                          >  1:4000807/1‑100 (MQ=255)                                         gCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGATGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACaa                                                        >  1:4804658/1‑100 (MQ=255)                                         gCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCGAGAGGTGCACTAAAATATAAAAGTACCCAAGATTTATTAAAACGATTAACaa                                                        >  1:280827/1‑100 (MQ=255)                                         gCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGacagagat                                                        >  1:6023308/1‑93 (MQ=255)                                         gCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACaa                                                        >  1:2831295/1‑100 (MQ=255)                                         gCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACaa                                                        >  1:6178373/1‑100 (MQ=255)                                         gCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACaa                                                        >  1:3123110/1‑100 (MQ=255)                                         gCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACaa                                                        >  1:2188734/1‑100 (MQ=255)                                         gCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACaa                                                        >  1:33149/1‑100 (MQ=255)                                         gCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACaa                                                        >  1:5291652/1‑100 (MQ=255)                                         gCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGACTAACaa                                                        >  1:963226/1‑100 (MQ=255)                                                     ataACATTGCCTTCTATTATATTCGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAg                                            >  1:3818549/1‑100 (MQ=255)                                                         cATTGCCTTCTATTATATTTGCGGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAATGATTAACAAGAATGAATGAAGCAtt                                        >  1:5413920/1‑100 (MQ=255)                                                          aTTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTACAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCAttt                                       >  1:594353/1‑100 (MQ=255)                                                          aTTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGACTGAATGTAgatcgg                                       >  1:4884482/1‑95 (MQ=255)                                                            tGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTaa                                     >  1:5072474/1‑100 (MQ=255)                                                               taccTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTc                                  >  1:4757984/4‑100 (MQ=255)                                                               cTTCTATTATATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTc                                  >  1:2836226/1‑100 (MQ=255)                                                                    attatATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACaa                             >  1:2340601/1‑100 (MQ=255)                                                                    attatATTTGCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACaa                             >  1:773608/1‑100 (MQ=255)                                                                             gCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATgg                    >  1:4042973/1‑100 (MQ=255)                                                                             gCTGACGAACCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATgg                    >  1:3562202/1‑100 (MQ=255)                                                                                 ctcaaCCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAAc                >  1:5194329/4‑100 (MQ=255)                                                                                    aaCCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCa             >  1:5418054/1‑100 (MQ=255)                                                                                     cactaCAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCAt            >  1:742028/5‑100 (MQ=255)                                                                                     aCCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCAt            >  1:1942342/1‑100 (MQ=255)                                                                                     aCCAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGCaagatcg            >  1:3919299/1‑95 (MQ=255)                                                                                      ccAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTGACGCaga           >  1:2444926/1‑98 (MQ=255)                                                                                      ccAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATg           >  1:2427262/1‑100 (MQ=255)                                                                                      ccAACAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATg           >  1:5619525/1‑100 (MQ=255)                                                                                       tcaCAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGa          >  1:1688986/3‑100 (MQ=255)                                                                                        ccagggTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTCTTATGGCAACGCATGAt         >  1:2281646/5‑100 (MQ=255)                                                                                        caCAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCacaga         >  1:5788331/2‑96 (MQ=255)                                                                                        aaCAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGAt         >  1:4653100/1‑100 (MQ=255)                                                                                         aCAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATc        >  1:5634145/1‑100 (MQ=255)                                                                                         aCAGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATc        >  1:599244/1‑100 (MQ=255)                                                                                          ctggTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATcc       >  1:182256/3‑100 (MQ=255)                                                                                          atggTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAATGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGccaagatc       >  1:6419647/3‑93 (MQ=255)                                                                                           aGGTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATCCt      >  1:3151177/1‑100 (MQ=255)                                                                                            tgTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAACGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATCCTg     >  1:5759201/2‑100 (MQ=255)                                                                                            tgTGCACTGGATTCTAAAAGAACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATCCTg     >  1:1651186/2‑100 (MQ=255)                                                                                            ggTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATCCTg     >  1:2740414/1‑100 (MQ=255)                                                                                             gTGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATCCTGt    >  1:3360989/1‑100 (MQ=255)                                                                                             ctGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATCCTGt    >  1:6277309/2‑100 (MQ=255)                                                                                             ctGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATCCTGt    >  1:222353/2‑100 (MQ=255)                                                                                              tGCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATCCTGtt   >  1:4568627/1‑100 (MQ=255)                                                                                               gCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATCCTGTTg  >  1:2768008/1‑100 (MQ=255)                                                                                               gCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATCCTGTTg  >  1:659756/1‑100 (MQ=255)                                                                                               gCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATCCTGTTg  >  1:3002814/1‑100 (MQ=255)                                                                                               gCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATCCTGTTg  >  1:347654/1‑100 (MQ=255)                                                                                               gCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATCCTGTTg  >  1:1704592/1‑100 (MQ=255)                                                                                               gCACTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATCCTGTTg  >  1:4472982/1‑100 (MQ=255)                                                                                                  |                                                                                                 CTCTGAATTGTCTGGTGGACAAAGGCAACGAACATCAGCTGCCAGAGCATTTATAACATTGCCTTCTATTATATTTGCTGACGAACCAACAGGTGCATTGGATTCTAAAAGTACTCAAGATTTATTAAAACGATTAACAAGAATGAATGAAGCATTTAAGTCTACAATTATTATGGTAACGCATGATCCTGTTG  >  NC_007793/721291‑721484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑