Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	714,167	G→A	intergenic (+390/‑66)	SAUSA300_RS03425 → / → SAUSA300_RS03430	hypothetical protein/membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	714,167	0	G	A	100.0%	128.6 / NA	46	intergenic (+390/‑66)	SAUSA300_RS03425/SAUSA300_RS03430	hypothetical protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/0);  total (46/0)

AAAAGCATAAATTTATAATTTTTTTAAATGTCATTGAACGTGATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGGCTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAAGGAGGTCTATTATTCACATGATGATGAATAAAGAAGCAACAAAAATTGGA  >  NC_007793/714074‑714265                                                                                              |                                                                                                   aaaaGCATAAATTTATAATTTTTTTAAATGTCATTGAACGTGATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCa                                                                                              >  1:686689/1‑100 (MQ=255)     gCATAAATTTATAATTTTTTTAAATGTCATTGAACGTGATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCaatta                                                                                          >  1:2343711/1‑100 (MQ=255)              tataA‑TTTTTTAAATGTCATTGAACGTGATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTa                                                                                >  1:5282514/1‑100 (MQ=255)               ataATTTTTTTAAATGTCATTGAACGTGATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTa                                                                                >  1:3850402/1‑100 (MQ=255)                         tAAATGTCATTGAACGTGATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATg                                                                      >  1:1413768/1‑100 (MQ=255)                          ttaTGTCATTGAACGTGATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGc                                                                     >  1:5044488/3‑100 (MQ=255)                          taaTGTCATTGAACGTGATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGc                                                                     >  1:2799007/2‑100 (MQ=255)                                 aTTGAACGTGATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACtgt                                                              >  1:2863137/1‑100 (MQ=255)                                   tGAACGTGATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGc                                                            >  1:4459388/1‑100 (MQ=255)                                    gAACGTGATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCa                                                           >  1:2310526/1‑100 (MQ=255)                                    gAACGTGATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCa                                                           >  1:3417756/1‑100 (MQ=255)                                     aaCGTGATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGTAATTAATTTTCAGTACGTTATAATGCACACTGTGCaa                                                          >  1:2364071/1‑100 (MQ=255)                                      aCGTGATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCaaa                                                         >  1:5944992/1‑100 (MQ=255)                                       agTGATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCaaaa                                                        >  1:2163006/2‑100 (MQ=255)                                          gATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAGATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTa                                                     >  1:2452307/1‑100 (MQ=255)                                          gATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTa                                                     >  1:10026/1‑100 (MQ=255)                                          gATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTa                                                     >  1:4446269/1‑100 (MQ=255)                                          gATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTa                                                     >  1:3473644/1‑100 (MQ=255)                                          gATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTa                                                     >  1:2439415/1‑100 (MQ=255)                                            gaaTGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAag                                                   >  1:4637659/2‑100 (MQ=255)                                             caTGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAagg                                                  >  1:4215049/2‑100 (MQ=255)                                             aaTGTGAATGGATTGAGCAGTTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGGGCAAAACTAagg                                                  >  1:5648933/1‑100 (MQ=255)                                             aaTGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTTagg                                                  >  1:5525753/1‑100 (MQ=255)                                             aaTGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAcgg                                                  >  1:1711054/1‑100 (MQ=255)                                             aaTGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAagg                                                  >  1:2540706/1‑100 (MQ=255)                                             aaTGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAagg                                                  >  1:1761751/1‑100 (MQ=255)                                             aaTGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAagg                                                  >  1:5367058/1‑100 (MQ=255)                                              ttgtgAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAagga                                                 >  1:5444111/2‑100 (MQ=255)                                               tgtgAATGGATTGAGCAATTTTGAAAAAGAGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAaggag                                                >  1:5068405/1‑100 (MQ=255)                                                gtgAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAgggaga                                               >  1:1923380/1‑99 (MQ=255)                                                gtgAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAaggagg                                               >  1:3512663/1‑100 (MQ=255)                                                gtgAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATCAaggaag                                               >  1:1580830/1‑98 (MQ=255)                                                 tgAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAAGGAGGt                                              >  1:5330658/1‑100 (MQ=255)                                                    aTGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAaggaggaaga                                           >  1:5848911/1‑96 (MQ=255)                                                      ggATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAAGGAGGTCtatt                                         >  1:6500281/1‑100 (MQ=255)                                                      ggATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAAGGAGGTCtatt                                         >  1:4333033/1‑100 (MQ=255)                                                         ttGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAAGGAGGTCTattatt                                      >  1:2345845/1‑100 (MQ=255)                                                          tGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAAGGAGGTCTATTATTc                                     >  1:4162699/1‑100 (MQ=255)                                                          tGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAAGGAGGTCTATTATTc                                     >  1:6036616/1‑100 (MQ=255)                                                          tGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAAAGAGGTCTATTATTc                                     >  1:4899834/1‑100 (MQ=255)                                                           taGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAAGGAGATcggaagagca                                    >  1:5639977/2‑91 (MQ=255)                                                           gAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAACTAAGGAGATcggaagagca                                    >  1:5246241/1‑91 (MQ=255)                                                           ctgCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAAGGAGGTCTATTATTca                                    >  1:422483/3‑100 (MQ=255)                                                           caGCAATTTTGAAAAAGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAAGGAGGTCTATTATTca                                    >  1:2511921/2‑100 (MQ=255)                                                                       aaaaGTGAAAAATAACCTATGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAAGGAGGTCTATTATTCACATGATGATGaa                        >  1:380636/1‑100 (MQ=255)                                                                                         aTGCGACTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAAGGAGGTCTATTATTCACATGATGATGAATAAAGAAGCAACAAAAAt      >  1:2801895/1‑100 (MQ=255)                                                                                             tgCTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAAGGAGGTCTATTATTCACATGATGATGAATAAAGAAGCAACAAAAATTGGa  >  1:6373120/2‑100 (MQ=255)                                                                                              |                                                                                                   AAAAGCATAAATTTATAATTTTTTTAAATGTCATTGAACGTGATAATGTGAATGGATTGAGCAATTTTGAAAAAGTGAAAAATAACCTATGCGGCTTGCAATTAATTTTCAGTACGTTATAATGCACACTGTGCAAAATTAAGGAGGTCTATTATTCACATGATGATGAATAAAGAAGCAACAAAAATTGGA  >  NC_007793/714074‑714265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑