Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	689,949	A→C	V547V (GTA→GTC)	SAUSA300_RS03310 →	sodium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	689,949	0	A	C	100.0%	72.6 / NA	29	V547V (GTA→GTC)	SAUSA300_RS03310	sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (29/0);  total (29/0)

AGGACAAATAACGAATAGTGTACTTGAAAACTATATGCGTTATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTATTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACGAGAGTGAACTCCGCATCTTCACTTAGTGTTACGGATAACTTAATGGAATTAAA  >  NC_007793/689853‑690041                                                                                                 |                                                                                              ggcccAAATAACGAATAGTGTACTTGAAAACTATATGCGTTATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGtctta                                                                                            >  1:245080/5‑100 (MQ=255)  ggACAAATAACGAATAGTGTACTTGAAAACTATATGCGTTATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGtcttat                                                                                           >  1:3575491/1‑100 (MQ=255)          aaCGAATAGTGTACTTGAAAACTATATGCGTTATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGt                                                                                   >  1:4549483/1‑100 (MQ=255)           ctatgtAGTGTACTTGAAAACTATATGCGTTATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTg                                                                                  >  1:2422833/6‑100 (MQ=255)                   ggtACTTGAAAACTATATGCGTTATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTtcatca                                                                          >  1:5313811/2‑98 (MQ=255)                    gtACTTGAAAACTATATGCGTTATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTaa                                                                         >  1:155177/1‑100 (MQ=255)                      aCTTGAAAACTATATGCGTTATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTaaaa                                                                       >  1:5013899/1‑100 (MQ=255)                         tGAAAACTATATGCGTTATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑Ga                                                                   >  1:5575650/1‑100 (MQ=255)                                gatatGCGTTATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAagatcgg                                                            >  1:6024995/2‑96 (MQ=255)                                 ctatGCGTTATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACa                                                           >  1:5893807/2‑100 (MQ=255)                                 atatGCGTTATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACa                                                           >  1:3260856/1‑100 (MQ=255)                                   atGCGTTATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACaaa                                                         >  1:5154853/1‑100 (MQ=255)                                       gTTATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACgag                                                     >  1:5501855/1‑100 (MQ=255)                                         tATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACagatcggaa                                                   >  1:3576338/1‑92 (MQ=255)                                         tATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACGagag                                                   >  1:5527762/1‑100 (MQ=255)                                         tATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACGagag                                                   >  1:2006095/1‑100 (MQ=255)                                         tATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACGagag                                                   >  1:1406512/1‑100 (MQ=255)                                          aTGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACGagaga                                                  >  1:4067220/1‑99 (MQ=255)                                          aTGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACGagaga                                                  >  1:248553/1‑99 (MQ=255)                                          aTGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACGAGAGt                                                  >  1:5162705/1‑100 (MQ=255)                                          aTGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCCTTATTAGATCGGAAGAGcacacgtctgaa                                                   >  1:5423589/1‑92 (MQ=255)                                            gCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACgagatcgg                                                >  1:1813405/1‑96 (MQ=255)                                                    aaCACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACGAGAGTGAACTCCGCa                                        >  1:221230/1‑100 (MQ=255)                                                     acacAGGTATATAGACAAGCATCATTAATAAGAAGCATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACGAGAGTGAACTCCGCAt                                       >  1:6120059/1‑100 (MQ=255)                                                     acacAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACGAGAGTGAACTCCGCAt                                       >  1:1199913/1‑100 (MQ=255)                                                     acacAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACGAGAGTGAACTCCGCAt                                       >  1:21539/1‑100 (MQ=255)                                                      cacaGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GCAGAGTACAAACGAGAGTGAACTCCGCATc                                      >  1:3508078/1‑100 (MQ=255)                                                       ccaGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACGAGAGTGAACTCCGCATCt                                     >  1:3508723/2‑100 (MQ=255)                                                        gaGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACGAGAGTGAACTCCGCATCtt                                    >  1:6163165/2‑100 (MQ=255)                                                        caGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTCTTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACGAGAGTGAACTCCGCATCtt                                    >  1:4556145/1‑100 (MQ=255)                                                                                          tGATTGTCTTATTACGAGGTGCTTTATGAAAAC‑GTAGAGTACAAACGAGAGTGAACTCCGCATCTTCACTTAGTGTTATGGATAAGTTAATGGAATtaaa  >  1:4682772/1‑100 (MQ=255)                                                                                                 |                                                                                              AGGACAAATAACGAATAGTGTACTTGAAAACTATATGCGTTATGCTGAAAGAACACAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTATTATTACGAGGTGCTTTATTAAAAC‑GAAGAGTACAAACGAGAGTGAACTCCGCATCTTCACTTAGTGTTACGGATAACTTAATGGAATTAAA  >  NC_007793/689853‑690041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑