Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	674,513	G→T	W80L (TGG→TTG)	SAUSA300_RS03225 →	haloacid dehalogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	674,513	0	G	T	100.0%	29.8 / NA	12	W80L (TGG→TTG)	SAUSA300_RS03225	haloacid dehalogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/0);  total (12/0)

AGATGACGATGAAGATAATGATAAACCTGATTTATATAAAGAAATCATTAAACGTTTCCATGTAGATAGGTTAACTTGGAAAGACTTATTTAATGATTTTGAAATGCATTTTTATCGTTATGTATTTCCTTATTACGATACTTTGTATACACTAGAAAAGCTATCGC  >  NC_007793/674436‑674602                                                                              |                                                                                          aGATGACGATGAAGATAATGATAAACCTGATTTATATAAAGAAATCATTAAACGTTTCCATGTAGATAGGTTAACTTTGAAAGACTTATTTAATGAtttt                                                                     >  1:1266788/1‑100 (MQ=255)                         aCCTGATTTATATAAAGAAATCATTAAACGTTTCCATGTAGATAGGTTAACTTTGAAAGACTTATTTAATGATTTTGAAATGCATTTTTATCGTTATGTa                                             >  1:607367/1‑100 (MQ=255)                                                       tttCCATGTAGATAGGTTAACTTTGAAAGACTTATTTAATGATTTTGAAATGCATTTTTATCGTTATGTATTTCCTTATTACGATACTTTGTATACACTa               >  1:5337836/1‑100 (MQ=255)                                                        ttCCATGTAGATAGGTTAACTTTGAAAGACTTATTTAATGATTTTGAAATGCATTTTTATCGTTATGTATTTCCTTATTACGATACTTTGTATACACTAg              >  1:5688827/1‑100 (MQ=255)                                                         tCCATGTAGATAGGTTAACTTTGAAAGACTTATTTAATGATTTTGAAATGCATTTTTATCGTTATGTATTTCCTTATTACGATACTTTGTATACACTAGa             >  1:4235880/1‑100 (MQ=255)                                                         tCCATGTAGATAGGTTAACTTTGAAAGACTTATTTAATGATTTTGAAATGCATTTTTATCGTTATGTATTTCCTTATTACGATACTTTGTATACACTAGa             >  1:4376675/1‑100 (MQ=255)                                                          gcATGTAGATAGGTTAACTTTGAAAGACTTATTTAATGATTTTGAAATGCATTTTTATCGTTATGTATTTCCTTATTACGATACTTTGTATACACTAGaa            >  1:1772151/2‑100 (MQ=255)                                                          ccATGTAGATAGGTTAACTTTGAAAGACTTATTTAATGATTTTGAAATGCATTTTTATAGTTATGTATTTCCTTATTACGATACTTTGTATACACTAGaa            >  1:4272899/1‑100 (MQ=255)                                                             tGTAGATAGGTTAACTTTGAAAGACTTATTTAATGATTTTGAAATGCATTTTTATCGTTATGTATTTCCTTATTACGATACTTTGTATACACTAGAAAAg         >  1:3158296/1‑100 (MQ=255)                                                                aGATAGGTTAACTTTGAAAGTCTTATTTAATGATTTTGAAATGCATTTTTATCGTTATGTATTTCCTTATTACGATACTTTGTATACACTAGAAAAGCTa      >  1:5448227/1‑100 (MQ=255)                                                                    aGGTTAACTTTGAAAGACTTATTTAATGATTTTGAAATGCATTTTTATCGTTATGTATTTCCTTATTACGATACTTTGTATACACTAGAAAAGCTATCGc  >  1:4261292/1‑100 (MQ=255)                                                                    aGGTTAACTTTGAAAGACTTATTTAATGATTTTGAAATGCATTTTTATCGTTATGTATTTCCTTATTACGATACTTTGTATACACTAGAAAAGCTATCGc  >  1:4299996/1‑100 (MQ=255)                                                                              |                                                                                          AGATGACGATGAAGATAATGATAAACCTGATTTATATAAAGAAATCATTAAACGTTTCCATGTAGATAGGTTAACTTGGAAAGACTTATTTAATGATTTTGAAATGCATTTTTATCGTTATGTATTTCCTTATTACGATACTTTGTATACACTAGAAAAGCTATCGC  >  NC_007793/674436‑674602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑