Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	673,027	C→T	R265C (CGT→TGT)	SAUSA300_RS03215 →	Vitamin B12 ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	673,027	0	C	T	100.0%	149.2 / NA	53	R265C (CGT→TGT)	SAUSA300_RS03215	Vitamin B12 ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (53/0);  total (53/0)

ATTAATTTCGACGGAAGCTAAGACAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACACGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCACGTATTGATGAAGGATTAAAAGAATTAAGAGATGCAATTTA  >  NC_007793/672933‑673112                                                                                                |                                                                                      agtAATTTCGACGGAAGCTAAGACAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTAtt                                                                                  >  1:1199846/3‑100 (MQ=255)         cGACGGAAGCTAAGACAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGt                                                                          >  1:1388538/1‑100 (MQ=255)               ccgCTAAGACAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATgg                                                                    >  1:4117238/3‑100 (MQ=255)                aGCTAAGACAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGt                                                                   >  1:2043751/1‑100 (MQ=255)                 ctcaaGACAAGATCAGATTATATGGATCTCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGtg                                                                  >  1:5124703/4‑100 (MQ=255)                   cctcccAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGtgat                                                                >  1:840427/6‑100 (MQ=255)                    aaGACAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTTGATTCAATAAAATTAATGCTGTCAAGAATACATGCATTGAAGTTGTAAATGGtgatg                                                               >  1:12827/1‑100 (MQ=255)                    aaGACAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGtgatg                                                               >  1:782361/1‑100 (MQ=255)                    aaGACAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGtgatg                                                               >  1:6095654/1‑100 (MQ=255)                    aaGACAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGtgatg                                                               >  1:3381656/1‑100 (MQ=255)                    aaGACAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGtgatg                                                               >  1:2770321/1‑100 (MQ=255)                    aaGACAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGtgatg                                                               >  1:328192/1‑100 (MQ=255)                    aaGACAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGtgatg                                                               >  1:2959794/1‑100 (MQ=255)                    aaGACAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGCGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGtgatg                                                               >  1:2941841/1‑100 (MQ=255)                     acccAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGtgatga                                                              >  1:1720034/4‑100 (MQ=255)                        cAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGt                                                           >  1:4066479/1‑100 (MQ=255)                         aaGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGtgagaagatc                                                          >  1:5461500/1‑93 (MQ=255)                          ttctCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTAt                                                         >  1:1704240/4‑100 (MQ=255)                          aGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTAt                                                         >  1:2853509/1‑100 (MQ=255)                           attCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATc                                                        >  1:5688415/3‑100 (MQ=255)                            ttCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCa                                                       >  1:5311194/2‑100 (MQ=255)                            ctCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCa                                                       >  1:5175034/2‑100 (MQ=255)                            ctCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCa                                                       >  1:705678/2‑100 (MQ=255)                            aTCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCa                                                       >  1:3816121/1‑100 (MQ=255)                              cAGATTATATGGATATCATCAAAAAAAAGAGGAGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGGGATGAAGTATCaa                                                      >  1:6585839/1‑100 (MQ=255)                              cAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCatg                                                     >  1:4948093/1‑98 (MQ=255)                              cAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCagg                                                     >  1:287670/1‑98 (MQ=255)                              cAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCGAg                                                     >  1:3612070/1‑100 (MQ=255)                              cAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAg                                                     >  1:3891000/1‑100 (MQ=255)                              cAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAg                                                     >  1:2123765/1‑100 (MQ=255)                              cAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAg                                                     >  1:2213711/1‑100 (MQ=255)                              cAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAg                                                     >  1:6034707/1‑100 (MQ=255)                              cAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAg                                                     >  1:1765289/1‑100 (MQ=255)                              cAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAg                                                     >  1:1549863/1‑100 (MQ=255)                              cAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAg                                                     >  1:223511/1‑100 (MQ=255)                              cAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAg                                                     >  1:861775/1‑100 (MQ=255)                              cAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAg                                                     >  1:228772/1‑100 (MQ=255)                              cAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAg                                                     >  1:2996047/1‑100 (MQ=255)                              cAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAg                                                     >  1:3550323/1‑100 (MQ=255)                                        ggATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCa                                           >  1:522057/1‑100 (MQ=255)                                           tatCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCACGt                                        >  1:1018444/1‑100 (MQ=255)                                            atcatc‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCACGTa                                       >  1:3145556/1‑100 (MQ=255)                                             tcatc‑aAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCGAGGCCAGATcggaagag                                      >  1:4858830/1‑93 (MQ=255)                                              catc‑aAAAAAAGGGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAGGACCAGATcggaagagc                                     >  1:5717801/1‑92 (MQ=255)                                              catc‑aAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGATcggaagagc                                     >  1:4535473/1‑92 (MQ=255)                                              accc‑aAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCACGTAtt                                     >  1:3206759/4‑100 (MQ=255)                                               atc‑aAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCACGTATtg                                    >  1:4052306/1‑100 (MQ=255)                                                     taaaaGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCACGTATTGATGaa                               >  1:1676100/2‑100 (MQ=255)                                                      caaaGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCACGTATTGATGAAg                              >  1:3956812/2‑100 (MQ=255)                                                        ccgagGTGGATTCAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCACGTATTGATGAAGGa                            >  1:5696576/3‑100 (MQ=255)                                                                    cAATAAAATTAATGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCACGTATTGATGAAGGATTAAAAGAATTa                >  1:6020013/1‑100 (MQ=255)                                                                             aaaTGCTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCACGTATTGATGAAGGATTAAAAGAGTTAAGAGAtcgg       >  1:5979879/2‑97 (MQ=255)                                                                                  cTGTCAAGAATACATGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCACGTATTGATGAAGGATTAAAAGAATTAAGAGATGCAATTta  >  1:2279906/1‑100 (MQ=255)                                                                                                |                                                                                      ATTAATTTCGACGGAAGCTAAGACAAGATCAGATTATATGGATATCATC‑AAAAAAAGAGGTGGATTCAATAAAATTAATGCTGTCAAGAATACACGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCACGTATTGATGAAGGATTAAAAGAATTAAGAGATGCAATTTA  >  NC_007793/672933‑673112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑