Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	662,424	G→T	G186V (GGT→GTT)	SAUSA300_RS03160 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	662,424	0	G	T	100.0%	15.8 / NA	8	G186V (GGT→GTT)	SAUSA300_RS03160	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/0);  total (8/0)

TTTGGCGGATTGACTATGCTATTCCTAGATGCACTATTATCAATGAGTTATCAAAACATAATAGTATATTTTGTTTGGATTGCAGTTATAATGGGTTTTTTTCTAGTTAATATAGCTTTAATTATAGATAAAAACATTCATGTCATACTTAAAAACCAATAGAAATTACCAAATTAAATT  >  NC_007793/662330‑662509                                                                                               |                                                                                      gttGGCGGATTGACTATGCTATTCCTAGATGCACTATTATCAATGAGTTATCAAAACATAATAGTATATTTTGTTTGGATTGCAGTTATAATGGtttttt                                                                                  >  1:4181159/2‑100 (MQ=255)  ttGGCGGATTGACTATGCTATTCCTAGATGCACTATTATCAATGAGTTATCAAAACATAATAGTATATTTTGTTTGGATTGCAGTTATAATGGTtttttt                                                                                 >  1:5692784/1‑100 (MQ=255)   tGGCGGATTGACTATGCTATTCCTAGATGCACTATTATCAATGAGTTATCAAAACATAACAGTATATTTTGTTTGGATTGCAGTTATAATGGtttttttt                                                                                >  1:817412/1‑100 (MQ=255)                aTGCTATTCCTAGATGCACTATTATCAATGAGTTATCAAAACATAATAGAATATTTTGTTTGGATTGCAGTTATAATGGTTTTTTTTCTAGTTAATATAg                                                                   >  1:6456929/1‑100 (MQ=255)                     aTTCCTAGATGCACTATTATCAATGAGTTATCAAAACATAATAGTATATTTTGTTTGGATTGCAGTTATAATGGTTTTTTTTCTAGTTAATATAGCTTTa                                                              >  1:3234238/1‑100 (MQ=255)                               gCACTATTATCAATGAGATATCAAAACATAATAGTATATTTTGTTTGGATTGCAGCTATCATGGTTTTTTTTCTAGCTAATATAGCTTTAATTATAGATa                                                    >  1:1399919/1‑100 (MQ=14)                               ccACTATTATCAATGAGTTATCAAAACATAATAGTATATTTTGTTTGGATTGCAGTTATAATGGTTTTTTTTCTAGTTAATATAGCTTTAATTATAGATa                                                    >  1:348911/2‑100 (MQ=255)                                     ctatCAATGAGTTATCAAAACATAATAGTATATTTTGTTTGGATTGCAGTTATAATGGTTTTTTTTCTAGTTAATATAGCTTTAATTATAGATAAAAACa                                              >  1:2855926/2‑100 (MQ=255)                                                             ataGTATATTTTGTTTGGATTGCAGTTATAATGGTTTTTTTTCTAGTTAATATAGCTTTAATTATAGATAAAAACATTCATGTCATACTTAAAAACCAAt                      >  1:4388098/1‑100 (MQ=255)                                                                                 tGCAGTTATAATGGTTTTTTTTCTAGTTAATATAGCTTTAATTATAGATAAAAACATTCATGTCATACTTAAAAACCAATAGAAATTACCaaattaaatt  >  1:6467276/1‑100 (MQ=255)                                                                                               |                                                                                      TTTGGCGGATTGACTATGCTATTCCTAGATGCACTATTATCAATGAGTTATCAAAACATAATAGTATATTTTGTTTGGATTGCAGTTATAATGGGTTTTTTTCTAGTTAATATAGCTTTAATTATAGATAAAAACATTCATGTCATACTTAAAAACCAATAGAAATTACCAAATTAAATT  >  NC_007793/662330‑662509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑