Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	658,463	A→T	intergenic (+28/‑268)	SAUSA300_RS03130 → / → SAUSA300_RS03135	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	658,463	0	A	T	100.0%	17.2 / NA	8	intergenic (+28/‑268)	SAUSA300_RS03130/SAUSA300_RS03135	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (4/4);  total (4/4)

TTTATGAATATGCATTCAATTATAGATAAAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTAATTTAGGGGTGGTAAACAGTGCTTTGTGAATCTAGACAAATTTATAAA  >  NC_007793/658373‑658511                                                                                           |                                                 tttatgAATATGCATTCAATTATAGATAAAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTTAATTAgggg                                         <  1:5497515/100‑1 (MQ=255) tttatgAATATGCATTCAATTATAGAT‑AAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTTAATTAggggg                                        <  1:5473406/100‑2 (MQ=255)   tatgAATATGCATTCAATTATAGATAAAAGGGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTTAATTAgggggg                                       <  1:1556743/100‑3 (MQ=255)         catGCATTCAATTATAGATAAAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTTAATTAgggggagatcgg                                 >  1:924405/2‑92 (MQ=255)            gCATTCAATTATAGATAAAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTTAATTAGGggtgagatcggaa                              >  1:3276630/1‑91 (MQ=255)            gCATTCAATTATAGATAAAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTTAATTAAGggttagatcggaa                              >  1:4876665/1‑90 (MQ=255)                     tataGATAAAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTTAATTAGGGGTGGTAAACAGTGCTTtgtgg                     <  1:4725735/100‑2 (MQ=255)                                        atatTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTTAATTAGGGGTGGTAAACAGTGCTTTGTGAATCTAGACAAATTTATaaa  >  1:5772006/1‑100 (MQ=255)                                                                                           |                                                 TTTATGAATATGCATTCAATTATAGATAAAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTAATTTAGGGGTGGTAAACAGTGCTTTGTGAATCTAGACAAATTTATAAA  >  NC_007793/658373‑658511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑