Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	656,348	G→T	S44I (AGT→ATT)	SAUSA300_RS03115 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	656,348	0	G	T	100.0%	46.4 / NA	19	S44I (AGT→ATT)	SAUSA300_RS03115	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/11);  total (8/11)

AATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGAATAGTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGC  >  NC_007793/656255‑656438                                                                                              |                                                                                           aaTATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCaa                                                                                      <  1:5987822/100‑1 (MQ=255)  atatAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCaaa                                                                                     <  1:5643333/100‑1 (MQ=255)              taAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGaaa                                                                         <  1:5477662/100‑1 (MQ=255)                    cAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATtagt                                                                   <  1:445919/100‑1 (MQ=255)                     aataatGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCC‑AAAAGTACGCGAAAATcagcag                                                                 <  1:414970/100‑1 (MQ=14)                                   cATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAgaagagaagatcggaag                                                    >  1:6110729/1‑93 (MQ=17)                                   cATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGaa                                                    <  1:3198990/100‑1 (MQ=255)                                                  aagtaCTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTc                                     >  1:2054448/1‑100 (MQ=255)                                                  aagtaCTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTc                                     >  1:3651293/1‑100 (MQ=255)                                                           gATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGca                            <  1:5127092/100‑2 (MQ=255)                                                           gATTGCTTATTTTTCGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCt                            <  1:4894662/100‑1 (MQ=25)                                                               gCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTaaaa                        >  1:1575939/1‑100 (MQ=255)                                                                   aTTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAACTTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAaataa                    >  1:6211251/1‑100 (MQ=37)                                                                        ttGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACtt               >  1:4785839/1‑100 (MQ=255)                                                                         tGCCCATGATTAATTGGTTTATTCCCATAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAATGTTTAAATATTGTCAAACCCGTTAAAAATAATACttt              >  1:2840561/1‑100 (MQ=25)                                                                          gCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTc             >  1:1141709/1‑100 (MQ=255)                                                                                   tAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGtt    <  1:5845160/100‑1 (MQ=255)                                                                                   tAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGta    <  1:2212307/100‑2 (MQ=255)                                                                                     aTTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGc  <  1:1784079/100‑1 (MQ=255)                                                                                              |                                                                                           AATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGAATAGTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGC  >  NC_007793/656255‑656438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑