Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 650,151 T→A V96E (GTA→GAA)  SAUSA300_RS03075 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007793650,1510TA99.0% 279.7 / ‑5.3 103V96E (GTA→GAA) SAUSA300_RS03075hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (96/6);  minor base G (1/0);  total (97/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 103 total aligned reads displayed.

TCGAAAAAGTTTCTCAACAATTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGTAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAGAAAAGCGAAAAGCACAACAATAAAGCCT  >  NC_007793/650058‑650211
                                                                                             |                                                                
tCGAAAAAGTTTCTCAACAATTAAAGAAACCACGTGATTCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑a                                                          <  1:4340649/100‑1 (MQ=255)
tCGAAAAAGTTTCTCAACAATTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑a                                                          <  1:3855221/100‑1 (MQ=255)
tCGAAAAAGTTTCTCAACAATTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑a                                                          <  1:4207315/100‑1 (MQ=255)
        atctagcaacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGaa                                                  >  1:4158491/7‑100 (MQ=255)
            ctcAACAATTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCt                                              >  1:633475/1‑100 (MQ=255)
            ctcAACAATTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCt                                              >  1:1722866/1‑100 (MQ=255)
             ccaacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGTA‑C‑AAGATCGGAAgagc                                             >  1:3233386/2‑97 (MQ=255)
             acaacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATCGGAAgagc                                             >  1:2027348/2‑97 (MQ=255)
              caacaaTTAAAGAAACCCCGTGATCCTGCAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGCAAGCTT‑a                                           >  1:6545640/1‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGGAGAG‑C‑AAGATCGGAAgagca                                            >  1:6115281/1‑96 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAGGAA‑C‑GAGATCGGAAgagca                                            >  1:4056067/1‑96 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAGGAA‑C‑AAGATCGGAAgagca                                            >  1:6390950/1‑96 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAATAA‑C‑AAGATCGGAAgagca                                            >  1:4222483/1‑96 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGGA‑C‑AAGATCGGAAgagca                                            >  1:5646086/1‑96 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGCA‑C‑AAGATCGGAAgagca                                            >  1:4073235/1‑96 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAC‑C‑AAGATCGGAAgagca                                            >  1:163450/1‑96 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAATC‑AAGATCGGAAgagc                                             >  1:25764/1‑97 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAATC‑AAGATCGGAAgagc                                             >  1:4045543/1‑97 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAAGC‑AAGATCGGAAgagc                                             >  1:2065993/1‑97 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CTAAGATCGGAAgagc                                             >  1:4308024/1‑97 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATCGGAAgagca                                            >  1:144421/1‑96 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATCGGAAgagca                                            >  1:6157248/1‑96 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGatcggatg                                            >  1:6078732/1‑93 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑a                                           >  1:3469652/1‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑a                                           >  1:2905987/1‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑a                                           >  1:5930292/1‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑a                                           >  1:5252939/1‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑a                                           >  1:5662476/1‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAAAA‑C‑AAGATCGGAAgagaa                                            >  1:800515/1‑96 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGAGTTTAAGAAAACGACATGAAGAACGTAAACGTCGAAAAGAAGAA‑C‑AAGATCGGAAgagca                                            >  1:1969753/1‑96 (MQ=255)
              caacaaTTAAAGAAACCACGTGACCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGCCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑a                                           >  1:1874829/1‑100 (MQ=255)
               aacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑aa                                          <  1:3894211/100‑1 (MQ=255)
                      aaaGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑aaaaa                                  >  1:643221/1‑100 (MQ=255)
                       aaGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAAt                                 >  1:1706277/1‑100 (MQ=255)
                        aGAAACCACGTGATCCTGAAGACGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAAtc                                >  1:375085/1‑99 (MQ=255)
                         gAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑aaaaagca                               >  1:55962/1‑97 (MQ=255)
                         gAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑aaaaagat                               >  1:3634364/1‑97 (MQ=255)
                         gAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAAtcg                               >  1:3465103/1‑98 (MQ=255)
                         gAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAAtaa                               >  1:5938601/1‑98 (MQ=255)
                          taaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑aaaaaaaaa                              >  1:3742177/2‑96 (MQ=255)
                          ctcccACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATAAg                              >  1:1778130/4‑100 (MQ=255)
                          caaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTaa                              >  1:3216575/2‑99 (MQ=255)
                          caaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAg                              >  1:6370736/2‑100 (MQ=255)
                          aaaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AGAAATTAg                              >  1:3442200/1‑100 (MQ=255)
                          aaaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTaa                              >  1:87149/1‑99 (MQ=255)
                          aaaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAg                              >  1:2159964/1‑100 (MQ=255)
                           tgccACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAA‑AAAAATAAga                             >  1:4413312/3‑100 (MQ=255)
                           caCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAga                             >  1:1436924/2‑100 (MQ=255)
                           caCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATCAga                             >  1:3479717/2‑100 (MQ=255)
                           aaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTGga                             >  1:1585205/1‑100 (MQ=255)
                           aaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAga                             >  1:1630859/1‑100 (MQ=255)
                           aaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAga                             >  1:5873535/1‑100 (MQ=255)
                            aCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgcg                            >  1:424949/1‑98 (MQ=255)
                            aCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaa                            >  1:2070389/1‑100 (MQ=255)
                            aCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaa                            >  1:5219992/1‑100 (MQ=255)
                             cgtgtggATCTTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATCAgaag                           <  1:1698240/94‑2 (MQ=255)
                             ccACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑aaaaaacgagaa                           >  1:5132055/1‑93 (MQ=255)
                               ctgTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AATAATTAgatcgg                         >  1:3478766/3‑96 (MQ=255)
                               ccGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaaaag                         >  1:1498946/2‑100 (MQ=255)
                               aCGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑aaaagatcagaaga                         >  1:3360594/1‑90 (MQ=255)
                               aCGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaaaag                         >  1:2738208/1‑100 (MQ=255)
                               aCGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATAAgaaaag                         >  1:1727689/1‑100 (MQ=255)
                                ggTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaaaaga                        >  1:3298587/2‑99 (MQ=255)
                                cGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTGgaaaaga                        >  1:3725194/1‑99 (MQ=255)
                                cGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATGAgaaaaga                        >  1:722180/1‑99 (MQ=255)
                                 gTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATCAgaaaagat                       >  1:5901573/1‑98 (MQ=255)
                                 ctGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAGAAAAAATGAgaaaaga                        >  1:5041600/2‑99 (MQ=255)
                                  tggtCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAggaaagatc                      >  1:574164/4‑97 (MQ=255)
                                  tGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATGAcaaagatcg                      >  1:2390447/1‑95 (MQ=255)
                                  tGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATAAgaaagatcg                      >  1:5131254/1‑95 (MQ=255)
                                   taTCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAAATAaaaagatcgg                     >  1:1376681/2‑94 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAGCGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATCAgaaaagatcg                     >  1:2725/1‑96 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTTAAAAA‑AAAAATGAgaaaagatc                      >  1:2704875/1‑97 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AGAAATTAgatcggaaga                     >  1:4007002/1‑92 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAGTTAgaagatcgga                     >  1:709376/1‑93 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTGgaaagatcgg                     >  1:3605471/1‑94 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAggagatcgga                     >  1:5378360/1‑91 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaagatcgga                     >  1:260237/1‑93 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATAAgaaaagatcg                     >  1:9066/1‑96 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAAGCAgaagatcgga                     >  1:1192591/1‑93 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAACTAgatcggaaga                     >  1:310301/1‑92 (MQ=255)
                                    ctCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaaaagcgaaa                    >  1:6259866/2‑100 (MQ=255)
                                     tCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaaaagatcgga                   >  1:3304701/1‑94 (MQ=255)
                                     cccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaaaagatcgga                   >  1:1118079/2‑94 (MQ=255)
                                      ccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTACACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaaagatcggaag                  >  1:3637003/1‑91 (MQ=255)
                                      ccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAACTCTgaaaagatcggaa                  >  1:3922922/1‑93 (MQ=255)
                                      ccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaacagatcggaa                  >  1:5658446/1‑93 (MQ=255)
                                      ccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaaagatcggaag                  >  1:5275760/1‑91 (MQ=255)
                                      ccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaaaagatcggaa                  >  1:348786/1‑93 (MQ=255)
                                      ccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaaaaacgaaaag                  >  1:484482/1‑100 (MQ=255)
                                      ccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATCAgaaaagatcggaa                  >  1:5120635/1‑93 (MQ=255)
                                      ccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAAGTAgaaagatcggaag                  >  1:4557954/1‑91 (MQ=255)
                                      ccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAAATAgaagatcggaagg                  >  1:4164271/1‑90 (MQ=255)
                                      ccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAA‑AAAAATTGgaaagatcggaag                  >  1:2373586/1‑91 (MQ=255)
                                       cTGCAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGAAAAAGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaaaagatcggaag                 >  1:6139466/1‑92 (MQ=255)
                                        tGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaaaagatcggaaga                >  1:1989966/1‑91 (MQ=255)
                                        tGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAgaaaagatcggaaga                >  1:584621/1‑91 (MQ=255)
                                        tGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAaaaaagatcggaaga                >  1:4870092/1‑91 (MQ=255)
                                        tGAAGAAGAAGAGCGATTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTGgaaaagatcggaaga                >  1:1947591/1‑91 (MQ=255)
                                                      gTTTAAGAAAACGACATGAAGAACGTAAACGGCGTAAAGAAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAGAAAAGCGAAAAGCACAACAATAAAGccg  <  1:2430152/100‑2 (MQ=255)
                                                                                             |                                                                
TCGAAAAAGTTTCTCAACAATTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGTAGAA‑C‑AAGATAGAAAGCTT‑AAAG‑AAAAATTAGAAAAGCGAAAAGCACAACAATAAAGCCT  >  NC_007793/650058‑650211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑