Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	635,213	A→G	K186K (AAA→AAG)	SAUSA300_RS02990 →	acetyl‑CoA acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	635,213	0	A	G	100.0%	19.2 / NA	9	K186K (AAA→AAG)	SAUSA300_RS02990	acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/1);  total (8/1)

GAAAATGTGGCCAAGATGTATGATGTTTCAAGAGAATTACAAGATGAATTTGCTTATCGAAGTCATCAATTGACAGCGGAAAATGTAAAAAATGGAAATATTTCTCAGGAAATATTACCTATAACCGTTAAAGGAGAAATATTCAACACTGATGAAAGTCTAAAATCACA  >  NC_007793/635124‑635293                                                                                          |                                                                                 gAAAATGTGGCCAAGATGTATGATGTTTCAAGAGAATTACAAGATGAATTTGCTTATCGAAGTCATCAATTGACAGCGGAAAATGTAAAGAATGGAAata                                                                        >  1:5062855/1‑100 (MQ=255)     aTGTGGCCAAGATGTATGATGTTTCAAGAGAATTACAAGATGAATTTGCTTATCGAAGTCATCAATTGACAGCGGAAAATGTAAAGAATGGAAATATTtc                                                                    >  1:2191361/1‑100 (MQ=255)         ggCCAAGATGTATGATGTTTCAAGAGAATTACAAGATGAATTTGCTTATCGAAGTCATCAATTGACAGCGGAAAATGTAAAGCATGGAAATATTTCTCAg                                                                >  1:611929/1‑100 (MQ=255)         ggCCAAGATGTATGATGTTTCAAGAGAATTACAAGATGAATTTGCTTATCGAAGTCATCAATTGACAGCGGAAAATGTAAAGAATGGAAATATTTCTCAg                                                                >  1:1777852/1‑100 (MQ=255)         ggCCAAGATGTATGATGTTTCAAGAGAATTACAAGATGAATTTGCTTATCGAAGTCATCAATTGACAGCGGAAAATGTAAAGAATGGAAATATTTCTCAg                                                                >  1:296098/1‑100 (MQ=255)                                gagaATTACAAGATGAATTTGCTTATCGAAGTCATCAATTGACAGCGGAAAATGTAAAGAATGGAAATATTTCTCAGGAAATATTACCTATAACCGTTaa                                         <  1:5709534/100‑1 (MQ=255)                                                                   caATTGACAGCGGAAAATGTAAAGAATGGAAATATTTCTCAGGAAATATTACCTATAACCGTTAAAGGAGAAATATTCAACACTGATGAAAGTCTAAAAt      >  1:1929171/1‑100 (MQ=255)                                                                       tGACAGCGGAAAATGTAAAGAATGGAAATATTTCTCAGGAAATATTACCTATAACCGTTAAAGGAGAAATATTCAACACTGATGAAAGTCTAAAATcaca  >  1:5652357/1‑100 (MQ=255)                                                                       tGACAGCGGAAAATGTAAAGAATGGAAATATTTCTCAGGAAATATTACCTATAACCGTTAAAGGAGAAATATTCAACACTGATGAAAGTCTAAAATcaca  >  1:910982/1‑100 (MQ=255)                                                                                          |                                                                                 GAAAATGTGGCCAAGATGTATGATGTTTCAAGAGAATTACAAGATGAATTTGCTTATCGAAGTCATCAATTGACAGCGGAAAATGTAAAAAATGGAAATATTTCTCAGGAAATATTACCTATAACCGTTAAAGGAGAAATATTCAACACTGATGAAAGTCTAAAATCACA  >  NC_007793/635124‑635293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑