Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 621,961 C→T D1022D (GAC→GAT)  SAUSA300_RS02925 → MSCRAMM family adhesin SdrE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007793621,9610CT91.4% 101.4 / 7.2 129D1022D (GAC→GAT) SAUSA300_RS02925MSCRAMM family adhesin SdrE
Reads supporting (aligned to +/- strand):  ref base C (10/1);  new base T (112/5);  total (123/6)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 132 total aligned reads displayed.

CAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGAC  >  NC_007793/621867‑622045
                                                                                              |                                                                                    
cagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGACAGCTActcagactcaga                                                                                 >  1:6481733/1‑100 (MQ=12)
     gcggacaGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCg                                                                            >  1:5862174/7‑100 (MQ=0)
     gcggacaGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCg                                                                            >  1:1891151/7‑100 (MQ=0)
       agcaggCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGCCAGCGAc                                                                          >  1:2530102/6‑100 (MQ=2)
        tacaGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGACTCAGACAGCGACt                                                                         >  1:6192799/4‑100 (MQ=11)
        cgcaGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACt                                                                         >  1:1426984/4‑100 (MQ=11)
         acaGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGACtc                                                                        >  1:4511459/3‑100 (MQ=2)
         acaGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACtc                                                                        >  1:5403252/3‑100 (MQ=11)
         acaGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACtc                                                                        >  1:5513990/3‑100 (MQ=11)
         acaGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACtc                                                                        >  1:5602615/3‑100 (MQ=11)
          tAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACtca                                                                       >  1:3827313/1‑100 (MQ=11)
           ggCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGAccaga                                                                      >  1:1190871/2‑96 (MQ=2)
           aGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACtcag                                                                      >  1:2806907/1‑100 (MQ=11)
            gCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACtcaga                                                                     >  1:2192110/1‑100 (MQ=11)
            gCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGAGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACtcaga                                                                     >  1:1391609/1‑100 (MQ=11)
                ctcagacgcagatAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGAGTCCTAACCAGACCGCGAcagacacgca                                                                 >  1:6419306/2‑91 (MQ=2)
                        cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGa                                                         >  1:3439626/1‑100 (MQ=255)
                         tgataGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAt                                                        >  1:3688683/5‑100 (MQ=11)
                         ggacAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGAt                                                        >  1:4468580/2‑100 (MQ=2)
                         ctacggCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGAt                                                        >  1:1001442/6‑100 (MQ=2)
                         agacAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAt                                                        >  1:4299145/1‑100 (MQ=11)
                         agacAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAt                                                        >  1:97277/1‑100 (MQ=11)
                         agacAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGAt                                                        >  1:3244581/1‑100 (MQ=2)
                          tactgggACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGCCGCAGATAGCGAtt                                                       >  1:4841311/7‑100 (MQ=2)
                          gacAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAtt                                                       >  1:2207721/1‑100 (MQ=11)
                          gacAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAtt                                                       >  1:3255634/1‑100 (MQ=11)
                           atggCGATTCAGAGTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTc                                                      <  1:3206698/97‑1 (MQ=11)
                           ataGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATTCAGACAGCGACTCAGACGCAGATAGCGATTc                                                      <  1:4488667/98‑1 (MQ=11)
                           ataGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTc                                                      <  1:5159941/98‑1 (MQ=11)
                            cAGCGACTCAGACGCAGATAGCGATTCTGATGTGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGATTca                                                     >  1:669942/1‑99 (MQ=2)
                            cAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGAtttc                                                     >  1:3389176/1‑98 (MQ=2)
                            cAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGATTca                                                     >  1:4487401/1‑99 (MQ=2)
                             ctactggcagattcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGGttaca                                                    >  1:1593562/8‑97 (MQ=0)
                             aGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGGGATTcag                                                    >  1:2182305/1‑98 (MQ=11)
                             aGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTcag                                                    >  1:247763/1‑98 (MQ=11)
                             aGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTcag                                                    >  1:6345410/1‑98 (MQ=11)
                                 cttcagatgcggacAGAGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGAtttcagatc                                                >  1:3782710/3‑93 (MQ=2)
                                    cagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCGGACTCAGa                                             >  1:771374/1‑100 (MQ=255)
                                    cagacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGa                                             >  1:1970806/7‑100 (MQ=0)
                                     cgacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGAt                                            >  1:4356651/6‑100 (MQ=2)
                                     agacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGAt                                            >  1:2708598/6‑100 (MQ=2)
                                        cgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGc                                         >  1:5633087/3‑100 (MQ=11)
                                          cagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                       >  1:4370790/1‑100 (MQ=11)
                                           agatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGTGAc                                      >  1:5549173/1‑100 (MQ=11)
                                                       tgatgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGAc                          >  1:5333587/8‑100 (MQ=11)
                                                       tgatgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGAc                          >  1:3318293/8‑100 (MQ=11)
                                                       agatgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGAc                          >  1:5376545/8‑100 (MQ=11)
                                                       acgtgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGAc                          >  1:371752/8‑100 (MQ=11)
                                                        gatgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACa                         >  1:2538671/7‑100 (MQ=2)
                                                        gatgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACa                         >  1:5058041/7‑100 (MQ=11)
                                                        gatgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACa                         >  1:3415496/7‑100 (MQ=11)
                                                        gatgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACa                         >  1:2668679/7‑100 (MQ=11)
                                                        gatgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACa                         >  1:2446083/7‑100 (MQ=11)
                                                        gatgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACa                         >  1:3108364/7‑100 (MQ=11)
                                                          tgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGACAGCGACTCTGATGCGGACAGc                       >  1:588482/5‑100 (MQ=11)
                                                           gcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAgtg                      >  1:1941969/4‑98 (MQ=1)
                                                           gcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAACg                      >  1:5306453/4‑100 (MQ=11)
                                                           ctggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCg                      >  1:228122/4‑100 (MQ=11)
                                                            tgtgcAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGa                     >  1:1666763/5‑100 (MQ=11)
                                                             tgACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGac                    >  1:4451957/2‑99 (MQ=11)
                                                             acaCAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAgatcg                    >  1:2927435/3‑96 (MQ=1)
                                                             aagcAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGac                    >  1:2264759/4‑99 (MQ=11)
                                                             aGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGac                    >  1:353538/1‑99 (MQ=11)
                                                              taCAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGact                   >  1:4649390/2‑98 (MQ=11)
                                                               ataGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTc                  >  1:5049745/3‑100 (MQ=2)
                                                                 aGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAg                >  1:2241666/1‑100 (MQ=11)
                                                                 aGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAg                >  1:4757380/1‑100 (MQ=11)
                                                                  gCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGa               >  1:1072787/1‑100 (MQ=11)
                                                                   actaccAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGAc              >  1:479611/6‑100 (MQ=2)
                                                                     aTTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGAcgc            <  1:4093712/100‑3 (MQ=2)
                                                                     aTTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGAcgc            <  1:1487784/100‑3 (MQ=2)
                                                                      ttCAGACGCGGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCa           >  1:3891958/1‑100 (MQ=2)
                                                                       tCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAg          >  1:6218680/1‑100 (MQ=2)
                                                                        cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGCTTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa         >  1:6553633/1‑100 (MQ=2)
                                                                        cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGCTTCAGACGCAGa         >  1:2006529/1‑100 (MQ=2)
                                                                        cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa         >  1:4773021/1‑100 (MQ=2)
                                                                        cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa         >  1:1058672/1‑100 (MQ=2)
                                                                        cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa         >  1:6626753/1‑100 (MQ=2)
                                                                        cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa         >  1:6079432/1‑100 (MQ=2)
                                                                        cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa         >  1:5729543/1‑100 (MQ=2)
                                                                        cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa         >  1:3557434/1‑100 (MQ=2)
                                                                        cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa         >  1:5578876/1‑100 (MQ=2)
                                                                         cgACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGAt        >  1:5559445/2‑100 (MQ=2)
                                                                         aGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGAt        >  1:6063745/1‑100 (MQ=2)
                                                                         aGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGAt        >  1:5467323/1‑100 (MQ=2)
                                                                          gACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATa       >  1:4232293/1‑100 (MQ=2)
                                                                          gACGCAGATAGCGACTCTGATGCGGACAGAGACTAAGATTCAGACAGAGATTCTGACGCCGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGaaa       >  1:4470891/1‑98 (MQ=2)
                                                                           acgcAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAg      >  1:2127051/4‑100 (MQ=2)
                                                                            tgcAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGTTGCGGACAGCGATTCAGACGCAGATAGc     >  1:2472605/3‑100 (MQ=2)
                                                                            cTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGc     >  1:4847407/1‑100 (MQ=255)
                                                                             gcAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCg    >  1:6487560/2‑100 (MQ=11)
                                                                             gcAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCg    >  1:435516/2‑100 (MQ=11)
                                                                             gcAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCg    >  1:3510038/2‑100 (MQ=11)
                                                                             ccAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCg    >  1:2854025/2‑100 (MQ=11)
                                                                              cAGATAGCGACTCTGCTGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCga   >  1:3691951/1‑100 (MQ=2)
                                                                              cAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCga   >  1:4225112/1‑100 (MQ=11)
                                                                              cAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCga   >  1:1768578/1‑100 (MQ=11)
                                                                              cAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCga   >  1:3173489/1‑100 (MQ=255)
                                                                              cAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCga   >  1:879034/1‑100 (MQ=255)
                                                                               aGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCGGACGCAGAtcggaag  >  1:1422355/1‑94 (MQ=2)
                                                                                              |                                                                                    
CAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGAC  >  NC_007793/621867‑622045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑