Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 621,944 T→G S1017A (TCA→GCA)  SAUSA300_RS02925 → MSCRAMM family adhesin SdrE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007793621,9440TG91.0% 89.4 / 15.1 133S1017A (TCA→GCA) SAUSA300_RS02925MSCRAMM family adhesin SdrE
Reads supporting (aligned to +/- strand):  ref base T (11/1);  new base G (116/5);  total (127/6)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01

Only 100 of 134 total aligned reads displayed.

CAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGC  >  NC_007793/621849‑622042
                                                                                               |                                                                                                  
 cgACGCAGATAGCGACTCTGATGCAGATAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTTAGACGCAGACAGCGATTCAGACGCAGAt                                                                                               >  1:2985775/2‑100 (MQ=2)
  gACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATa                                                                                              >  1:5731349/1‑100 (MQ=2)
  gACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATa                                                                                              >  1:133884/1‑100 (MQ=2)
  atggcAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATa                                                                                              >  1:1555198/5‑100 (MQ=2)
   aCTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAg                                                                                             >  1:4266002/1‑100 (MQ=35)
      cAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCga                                                                                          >  1:4404503/1‑100 (MQ=35)
        gctAGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCgatt                                                                                        >  1:5542670/3‑98 (MQ=2)
           aGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCgattcag                                                                                     >  1:1423845/1‑100 (MQ=2)
           aGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCgattcag                                                                                     >  1:4702757/1‑100 (MQ=2)
              tcttcagatgcggacAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATCCTGATGCGGACAGCGATTCAGACGCAGATAGCgattcagatt                                                                                  >  1:3912946/4‑98 (MQ=2)
                  catattcagatAGCGATTCTGATGCGGACAGCGATTCATACGCAGATAGCGATTTTGCTGCGGACAGCGATTCATCCGCAGATAGCGCttcagattcaga                                                                              >  1:2801310/4‑100 (MQ=2)
                  cagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGACAGCTActcagactcaga                                                                              >  1:6481733/1‑100 (MQ=12)
                       gcggacaGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCg                                                                         >  1:3466294/7‑100 (MQ=0)
                       gcggacaGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCg                                                                         >  1:1891151/7‑100 (MQ=0)
                         agcaggCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGCCAGCGAc                                                                       >  1:2530102/6‑100 (MQ=2)
                          tacaGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGACTCAGACAGCGACt                                                                      >  1:6192799/4‑100 (MQ=11)
                          cgcaGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACt                                                                      >  1:1426984/4‑100 (MQ=11)
                           acaGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACtc                                                                     >  1:5602615/3‑100 (MQ=11)
                           acaGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACtc                                                                     >  1:2373908/3‑100 (MQ=11)
                           acaGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACtc                                                                     >  1:5513990/3‑100 (MQ=11)
                           acaGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACtc                                                                     >  1:5403252/3‑100 (MQ=11)
                            tAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACtca                                                                    >  1:3827313/1‑100 (MQ=11)
                             aGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACtcag                                                                   >  1:2806907/1‑100 (MQ=11)
                              gCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGAGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACtcaga                                                                  >  1:1391609/1‑100 (MQ=11)
                                 cttCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATtcagactc                                                               >  1:3584076/2‑100 (MQ=11)
                                  ctcagacgcagatAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGAGTCCTAACCAGACCGCGAcagacacgca                                                              >  1:6419306/2‑91 (MQ=2)
                                          cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGa                                                      >  1:3439626/1‑100 (MQ=255)
                                           tgataGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGCCTCAGACGCAGATAGCGAt                                                     >  1:5237012/5‑100 (MQ=11)
                                           tgataGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAt                                                     >  1:3688683/5‑100 (MQ=11)
                                           ggacAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGAt                                                     >  1:4468580/2‑100 (MQ=2)
                                           ctacggCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGAt                                                     >  1:1001442/6‑100 (MQ=2)
                                           agatAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAt                                                     >  1:2157043/1‑100 (MQ=11)
                                           agacAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAt                                                     >  1:4299145/1‑100 (MQ=11)
                                           agacAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAt                                                     >  1:97277/1‑100 (MQ=11)
                                           agacAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGAt                                                     >  1:3244581/1‑100 (MQ=2)
                                            tactgggACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGCCGCAGATAGCGAtt                                                    >  1:4841311/7‑100 (MQ=2)
                                            gacAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAtt                                                    >  1:2207721/1‑100 (MQ=11)
                                            cttaGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGAtt                                                    >  1:3326007/4‑100 (MQ=2)
                                             atggCGATTCAGAGTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTc                                                   <  1:3206698/97‑1 (MQ=11)
                                             ataGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATTCAGACAGCGACTCAGACGCAGATAGCGATTc                                                   <  1:4488667/98‑1 (MQ=11)
                                             ataGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTc                                                   <  1:5159941/98‑1 (MQ=11)
                                              cAGCGACTCAGACGCAGATAGCGATTCTGATGTGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGATTca                                                  >  1:669942/1‑99 (MQ=2)
                                              cAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGAtttc                                                  >  1:3389176/1‑98 (MQ=2)
                                              cAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGATTca                                                  >  1:4487401/1‑99 (MQ=2)
                                              cAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGATTca                                                  >  1:1282990/1‑99 (MQ=2)
                                               ctactggcagattcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGGttaca                                                 >  1:1593562/8‑97 (MQ=0)
                                               aGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGGGATTcag                                                 >  1:2182305/1‑98 (MQ=11)
                                               aGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTcag                                                 >  1:2948191/1‑98 (MQ=11)
                                                   cttcagatgcggacAGAGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGAtttcagatc                                             >  1:3782710/3‑93 (MQ=2)
                                                      cagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCGGACTCAGa                                          >  1:771374/1‑100 (MQ=255)
                                                       cgacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGAt                                         >  1:4356651/6‑100 (MQ=2)
                                                       agacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGAt                                         >  1:2708598/6‑100 (MQ=2)
                                                        acttcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGCTAGCGATTCAGAGTCAGACAGCGACTCAGACGCAGATAGCGATTCAGatcggaaga                                        >  1:6134209/3‑92 (MQ=2)
                                                          cgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGc                                      >  1:5633087/3‑100 (MQ=11)
                                                          cgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGc                                      >  1:3236714/3‑100 (MQ=11)
                                                            cagaaAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCGGACGCAGATAGCGa                                    >  1:4786048/1‑100 (MQ=11)
                                                             agatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGTGAc                                   >  1:5549173/1‑100 (MQ=11)
                                                                  tcaccagtgACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAATCAGATTCAGATAGAGATTCGGACCCAGATAGCGACTCAGa                              >  1:4479657/9‑100 (MQ=17)
                                                                         tgatgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGAc                       >  1:3318293/8‑100 (MQ=11)
                                                                         tgatgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGAc                       >  1:1946525/8‑100 (MQ=11)
                                                                         agatgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGAc                       >  1:5376545/8‑100 (MQ=11)
                                                                          gatgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACa                      >  1:2538671/7‑100 (MQ=2)
                                                                          gatgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACa                      >  1:2446083/7‑100 (MQ=11)
                                                                          gatgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACa                      >  1:5058041/7‑100 (MQ=11)
                                                                           ctgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAg                     >  1:5866427/6‑100 (MQ=1)
                                                                            tgcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGACAGCGACTCTGATGCGGACAGc                    >  1:588482/5‑100 (MQ=11)
                                                                             gcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAgtg                   >  1:1941969/4‑98 (MQ=1)
                                                                             gcggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAACg                   >  1:5306453/4‑100 (MQ=11)
                                                                             ctggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCg                   >  1:228122/4‑100 (MQ=11)
                                                                              tgtgcAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGa                  >  1:1666763/5‑100 (MQ=11)
                                                                              cAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGGCAGCGa                  >  1:1444034/1‑100 (MQ=255)
                                                                              cAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGa                  >  1:6416007/1‑100 (MQ=255)
                                                                               tgACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGac                 >  1:4451957/2‑99 (MQ=11)
                                                                               gcccAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGac                 >  1:5386500/4‑99 (MQ=11)
                                                                               acaCAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAgatcg                 >  1:3733524/3‑96 (MQ=1)
                                                                               acaCAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAgatcg                 >  1:2927435/3‑96 (MQ=1)
                                                                               aGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGac                 >  1:353538/1‑99 (MQ=11)
                                                                                taCAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGact                >  1:4649390/2‑98 (MQ=11)
                                                                                gACAGCGATTCGGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGact                >  1:2226404/1‑98 (MQ=11)
                                                                                gACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAgatcgg                >  1:3892437/1‑95 (MQ=1)
                                                                                 ataGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTc               >  1:5049745/3‑100 (MQ=2)
                                                                                 aCAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCAATTc               <  1:2498155/100‑1 (MQ=255)
                                                                                   aGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAg             >  1:2241666/1‑100 (MQ=11)
                                                                                   aGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAg             >  1:4757380/1‑100 (MQ=11)
                                                                                    gCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGa            >  1:1072787/1‑100 (MQ=11)
                                                                                     actaccAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGAc           >  1:479611/6‑100 (MQ=2)
                                                                                       aTTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGAcgc         <  1:4093712/100‑3 (MQ=2)
                                                                                         tCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAg       >  1:6218680/1‑100 (MQ=2)
                                                                                          cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGCTTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa      >  1:6553633/1‑100 (MQ=2)
                                                                                          cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGCTTCAGACGCAGa      >  1:2006529/1‑100 (MQ=2)
                                                                                          cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa      >  1:5729543/1‑100 (MQ=2)
                                                                                          cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa      >  1:5578876/1‑100 (MQ=2)
                                                                                          cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa      >  1:4316075/1‑100 (MQ=2)
                                                                                          cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa      >  1:6626753/1‑100 (MQ=2)
                                                                                          cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa      >  1:3557434/1‑100 (MQ=2)
                                                                                          cAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa      >  1:1058672/1‑100 (MQ=2)
                                                                                           cgACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGAt     >  1:5559445/2‑100 (MQ=2)
                                                                                           aGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGAt     >  1:6063745/1‑100 (MQ=2)
                                                                                           aGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCAATTCAGACGCAGAt     >  1:4887830/1‑100 (MQ=2)
                                                                                            gACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATa    >  1:4232293/1‑100 (MQ=2)
                                                                                               |                                                                                                  
CAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGC  >  NC_007793/621849‑622042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑