Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 621,926 T→G S1011A (TCA→GCA)  SAUSA300_RS02925 → MSCRAMM family adhesin SdrE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007793621,9260TG93.7% 206.5 / 18.9 207S1011A (TCA→GCA) SAUSA300_RS02925MSCRAMM family adhesin SdrE
Reads supporting (aligned to +/- strand):  ref base T (12/1);  new base G (191/3);  total (203/4)
Fisher's exact test for biased strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

Only 100 of 212 total aligned reads displayed.

ACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGA  >  NC_007793/621828‑622014
                                                                                                  |                                                                                        
attctgATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTc                                                                                         <  1:3176596/95‑1 (MQ=2)
accctgATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTc                                                                                         >  1:5399621/6‑100 (MQ=2)
  acAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAg                                                                                       >  1:1393818/2‑100 (MQ=11)
   cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGa                                                                                      >  1:1027961/1‑100 (MQ=11)
   cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGa                                                                                      >  1:4690924/1‑100 (MQ=11)
   cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGa                                                                                      >  1:5594363/1‑100 (MQ=11)
   cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGa                                                                                      >  1:1467790/1‑100 (MQ=11)
   cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGa                                                                                      >  1:5853524/1‑100 (MQ=11)
   cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGa                                                                                      >  1:6589410/1‑100 (MQ=11)
    gccaccagACAGCGATTCAGACGCAGATAGCGATTCAGCTTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGat                                                                                     >  1:4200339/8‑99 (MQ=11)
     gATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCGGACGCAGatc                                                                                    >  1:5293195/1‑98 (MQ=2)
      ttgcGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGAAAGCGATTCAGATTCAGATAGAGATTCTGATGCGGACAg                                                                                   >  1:3923908/4‑100 (MQ=2)
      atgcGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAg                                                                                   >  1:1110530/4‑100 (MQ=2)
      atgcGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAg                                                                                   >  1:1075677/4‑100 (MQ=2)
      atgcGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAg                                                                                   >  1:2836576/4‑100 (MQ=2)
       tgcGGACAGCGATTCAGACGCAGATAGCGATTCCGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGc                                                                                  >  1:4015031/3‑100 (MQ=2)
       tgcGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGc                                                                                  >  1:6323408/3‑100 (MQ=2)
       gtcagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGCTAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGc                                                                                  >  1:4804179/5‑100 (MQ=1)
        tcagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCg                                                                                 >  1:728985/4‑100 (MQ=1)
        tcagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCg                                                                                 >  1:231901/4‑100 (MQ=1)
        gcGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCg                                                                                 >  1:2702377/2‑100 (MQ=2)
        gcGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCg                                                                                 >  1:1636486/2‑100 (MQ=2)
        gcGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCg                                                                                 >  1:5882540/2‑100 (MQ=2)
        gcGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGACGCAGACAGCg                                                                                 >  1:3218359/2‑100 (MQ=11)
        gcGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGACGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGTAGACAGCg                                                                                 >  1:2515169/2‑100 (MQ=11)
        ccGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCg                                                                                 >  1:5333929/2‑100 (MQ=2)
         cagACAGCGCTTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGatcggaa                                                                                >  1:3734456/3‑94 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCGGACGCAGATAGCGATTCCGACGCAGATAGCGa                                                                                >  1:4870185/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCGGACGCAGatcggaa                                                                                >  1:2671535/3‑94 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGatcggaa                                                                                >  1:121195/3‑94 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGCTAGCGa                                                                                >  1:3644647/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:5484034/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:4316188/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:42628/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:2130163/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:1391311/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:4565912/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:385606/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:1997575/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:1284831/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:6382957/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:3508215/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:4728414/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:6404269/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:3361129/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:1104702/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:2422558/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:3110539/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:2911111/3‑100 (MQ=11)
         cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                                                                >  1:2391650/3‑100 (MQ=11)
         cGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACTCATATAGCGATTCGTATGCGGACAGCGa                                                                                >  1:960637/1‑100 (MQ=2)
         cGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGa                                                                                >  1:6530757/1‑100 (MQ=2)
         cGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGa                                                                                >  1:2013449/1‑100 (MQ=2)
         cGGACAGCGATTCAGACGCAGATAGCGATTCAGATCCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCGGACGCAGatcggaa                                                                                >  1:3103734/1‑94 (MQ=11)
          tgACAGCGATTCAGACGCAGATAGCGATTCAGGTTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGac                                                                               >  1:164874/2‑99 (MQ=11)
          tgACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGac                                                                               >  1:287046/2‑99 (MQ=11)
          ggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGAt                                                                               >  1:1651506/1‑100 (MQ=2)
          agACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGTTTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGac                                                                               >  1:1099777/2‑99 (MQ=11)
          agACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCTGACGCAGATAGCGATTCAGACGCAGATAGCGac                                                                               >  1:4093735/2‑99 (MQ=11)
          agACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGac                                                                               >  1:2148924/2‑99 (MQ=11)
          agACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGac                                                                               >  1:176495/2‑99 (MQ=11)
           cccAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGatcggaaga                                                                              >  1:2565443/3‑92 (MQ=11)
           caCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGAGTCAGATTCAGATAGCGATTCGGATGCGGACAGCGAtt                                                                              >  1:6076087/2‑100 (MQ=11)
             cAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCa                                                                            >  1:3905836/1‑100 (MQ=2)
              aGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGATTCAGACTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAg                                                                           >  1:5515732/1‑100 (MQ=11)
                 ttgccAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGCCGCGGACAGCGATTCAGAcg                                                                        >  1:1921258/5‑99 (MQ=2)
                      cgACGCAGATAGCGACTCTGATGCAGATAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTTAGACGCAGACAGCGATTCAGACGCAGAt                                                                   >  1:2985775/2‑100 (MQ=2)
                       gACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATa                                                                  >  1:5070029/1‑100 (MQ=35)
                       gACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATa                                                                  >  1:5731349/1‑100 (MQ=2)
                       atggcAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATa                                                                  >  1:1555198/5‑100 (MQ=2)
                           cAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCga                                                              >  1:4404503/1‑100 (MQ=35)
                             gctAGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCgatt                                                            >  1:5542670/3‑98 (MQ=2)
                                   tcttcagatgcggacAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATCCTGATGCGGACAGCGATTCAGACGCAGATAGCgattcagatt                                                      >  1:3912946/4‑98 (MQ=2)
                                   ctttcagatgcggacAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCgattcagatt                                                      >  1:4293655/4‑98 (MQ=2)
                                       catattcagatAGCGATTCTGATGCGGACAGCGATTCATACGCAGATAGCGATTTTGCTGCGGACAGCGATTCATCCGCAGATAGCGCttcagattcaga                                                  >  1:2801310/4‑100 (MQ=2)
                                       cagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGACAGCTActcagactcaga                                                  >  1:6481733/1‑100 (MQ=12)
                                            gcggacaGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCg                                             >  1:3466294/7‑100 (MQ=0)
                                            gcggacaGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCg                                             >  1:1891151/7‑100 (MQ=0)
                                              agcaggCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGCCAGCGAc                                           >  1:2530102/6‑100 (MQ=2)
                                               tacaGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGACTCAGACAGCGACt                                          >  1:6192799/4‑100 (MQ=11)
                                               cgcaGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACt                                          >  1:1426984/4‑100 (MQ=11)
                                                acaGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGACtc                                         >  1:4511459/3‑100 (MQ=2)
                                                               cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGa                          >  1:3439626/1‑100 (MQ=255)
                                                                tgataGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGCCTCAGACGCAGATAGCGAt                         >  1:5237012/5‑100 (MQ=11)
                                                                ggacAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGAt                         >  1:4468580/2‑100 (MQ=2)
                                                                ctacggCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGAt                         >  1:1001442/6‑100 (MQ=2)
                                                                agatAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAt                         >  1:5608562/1‑100 (MQ=11)
                                                                agatAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAt                         >  1:2157043/1‑100 (MQ=11)
                                                                 tactgggACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGCCGCAGATAGCGAtt                        >  1:4841311/7‑100 (MQ=2)
                                                                 gacAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAtt                        >  1:2207721/1‑100 (MQ=11)
                                                                 gacAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAtt                        >  1:3255634/1‑100 (MQ=11)
                                                                 cacAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGAtt                        >  1:5552621/2‑100 (MQ=2)
                                                                   cAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGAtttc                      >  1:3389176/1‑98 (MQ=2)
                                                                   cAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGATTca                      >  1:4487401/1‑99 (MQ=2)
                                                                    aGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGGGATTcag                     >  1:2182305/1‑98 (MQ=11)
                                                                        cttcagatgcggacAGAGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGAtttcagatc                 >  1:3782710/3‑93 (MQ=2)
                                                                           cagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCGGACTCAGa              >  1:771374/1‑100 (MQ=255)
                                                                            cgacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGAt             >  1:4356651/6‑100 (MQ=2)
                                                                             acttcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGCTAGCGATTCAGAGTCAGACAGCGACTCAGACGCAGATAGCGATTCAGatcggaaga            >  1:6134209/3‑92 (MQ=2)
                                                                               cgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGc          >  1:3236714/3‑100 (MQ=11)
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ACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGA  >  NC_007793/621828‑622014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑