Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 621,865 C→T D990D (GAC→GAT)  SAUSA300_RS02925 → MSCRAMM family adhesin SdrE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007793621,8650CT96.5% 713.3 / 14.5 810D990D (GAC→GAT) SAUSA300_RS02925MSCRAMM family adhesin SdrE
Reads supporting (aligned to +/- strand):  ref base C (25/3);  new base T (770/7);  total (800/10)
Fisher's exact test for biased strand distribution p-value = 3.85e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 813 total aligned reads displayed.

AGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAG  >  NC_007793/621766‑621959
                                                                                                   |                                                                                              
           cagatgcggacagcgattcagacgcagacagcgattcagacgcaGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGACtcagattcaga                                                                                     >  1:3098806/9‑100 (MQ=2)
           cagacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcaga                                                                                     >  1:2079915/1‑100 (MQ=2)
           cagacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcaga                                                                                     >  1:6069510/1‑100 (MQ=2)
           cagacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcaga                                                                                     >  1:4209105/1‑100 (MQ=2)
            tgacgcagatagCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcggac                                                                                    >  1:3501881/2‑94 (MQ=0)
            cgacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcagcc                                                                                    >  1:1690216/2‑98 (MQ=2)
             gacgcagatagCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcggaca                                                                                   >  1:1007088/1‑93 (MQ=0)
             gacgcagatagCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcggaca                                                                                   >  1:4040136/1‑93 (MQ=0)
              cagcagatagCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAg                                                                                  >  1:4525817/4‑100 (MQ=2)
                      agcgattcagatgcgGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctg                                                                          >  1:5314054/4‑98 (MQ=1)
                          attcagatgcggACAGCTATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGAGATtgagatgc                                                                      >  1:3449421/1‑97 (MQ=2)
                           ctcagattccgaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGCTTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcg                                                                     >  1:3630828/2‑96 (MQ=2)
                           cccagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGCTtctgacgcg                                                                     >  1:6518824/3‑97 (MQ=11)
                            ctagattcagaCAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcag                                                                    >  1:6122736/3‑100 (MQ=11)
                            atctatgcggaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcag                                                                    >  1:4236/10‑100 (MQ=2)
                             ctgatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcaga                                                                   >  1:2937919/9‑100 (MQ=2)
                             ccgatgcggaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGATtctgacgcaga                                                                   >  1:552400/9‑100 (MQ=0)
                             cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                                                   >  1:3813640/1‑94 (MQ=11)
                             cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                                                   >  1:2626804/1‑94 (MQ=11)
                             cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                                                   >  1:2545974/1‑94 (MQ=11)
                             cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                                                   >  1:5659135/1‑94 (MQ=11)
                             cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                                                   >  1:881015/1‑94 (MQ=11)
                             cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                                                   >  1:1938930/1‑94 (MQ=11)
                             cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGACGCGGACAGCGATTAAGACGCTCATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                                                   >  1:2287020/1‑94 (MQ=2)
                             cagattcagaCAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                                                   >  1:3398899/1‑100 (MQ=11)
                             cagacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                                                   >  1:4341591/7‑100 (MQ=0)
                             cagacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                                                   >  1:3266920/7‑100 (MQ=0)
                              tgatgcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATtcagacgcagat                                                                  >  1:4421175/8‑99 (MQ=2)
                              cgatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcagat                                                                  >  1:5927169/8‑99 (MQ=2)
                              cgacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCAATtcagacgcagat                                                                  >  1:4786295/6‑99 (MQ=2)
                              agacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGTGATTCTGATGCGGACAGCGATtcagacgcagat                                                                  >  1:930333/6‑99 (MQ=0)
                               tacgcagaTAGCGACTCTGATGCGGACAGCGACTCAGATTCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACa                                                                 >  1:1802516/5‑100 (MQ=11)
                               gatgcggaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagata                                                                 >  1:5143488/7‑98 (MQ=2)
                               gatgcggaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagata                                                                 >  1:4155133/7‑98 (MQ=2)
                               gatgcggaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagata                                                                 >  1:6187953/7‑98 (MQ=2)
                               gatgcggaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagata                                                                 >  1:3060999/7‑98 (MQ=2)
                               gatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcagata                                                                 >  1:312279/7‑98 (MQ=2)
                                gtgcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATCCAGACAGCGTTTCTGATGCGGACAg                                                                >  1:4841777/6‑100 (MQ=2)
                                 ctcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGc                                                               >  1:722342/2‑100 (MQ=11)
                                  tcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCg                                                              >  1:5018285/1‑100 (MQ=11)
                                  tcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCAGACAGCGATTCTGACGCGGACAGCg                                                              >  1:6580672/1‑100 (MQ=2)
                                  tcagaCAGCGATTCAGACACAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCg                                                              >  1:1899575/1‑100 (MQ=11)
                                   tggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                             >  1:5715436/3‑100 (MQ=2)
                                   cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGCCAGCGa                                                             >  1:1636687/1‑100 (MQ=2)
                                   cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGcag                                                             >  1:6007584/1‑98 (MQ=2)
                                   cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                             >  1:3547800/1‑100 (MQ=11)
                                   cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                             >  1:6397421/1‑100 (MQ=11)
                                   cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                             >  1:4941180/1‑100 (MQ=11)
                                   cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                             >  1:1995082/1‑100 (MQ=11)
                                   cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                             >  1:5395213/1‑100 (MQ=11)
                                    tgaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGTTTCAGATAGCGATTCTGATGCGGACAGCGat                                                            >  1:2506706/2‑99 (MQ=2)
                                    atgcAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGat                                                            >  1:1250653/4‑99 (MQ=2)
                                     tgcAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGTCAGCGatt                                                           >  1:2876723/3‑98 (MQ=2)
                                      ccAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagatgtcgattc                                                          >  1:2760218/2‑91 (MQ=2)
                                       gtgCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGACtca                                                         >  1:666779/3‑100 (MQ=2)
                                               cgctggcagatAGCGACTCTGATGCGGACAGCGCCTCAGACGCAGATAGCGACTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACtcagattcaga                                                 >  1:3729352/7‑100 (MQ=2)
                                               ccgacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                                 >  1:2193026/7‑100 (MQ=2)
                                               cagacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                                 >  1:6434524/7‑100 (MQ=2)
                                                cgacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagat                                                >  1:1077778/6‑100 (MQ=0)
                                                  ccgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAg                                              >  1:1196543/4‑100 (MQ=2)
                                                    gcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCg                                            >  1:4293703/2‑100 (MQ=11)
                                                    gcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCg                                            >  1:1144248/2‑100 (MQ=11)
                                                     cagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGa                                           >  1:5253057/1‑100 (MQ=11)
                                                      agatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGACTCAGACGCAGATAGCGAt                                          >  1:4385546/1‑100 (MQ=11)
                                                      agatAGCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGACTCAGACGCAGATAGCGACTATGATACGGACAGCGACTCAGATTCAGACAGCGAt                                          >  1:2687747/1‑100 (MQ=2)
                                                      acatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGac                                          >  1:1276758/3‑99 (MQ=2)
                                                       gacaGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAtt                                         >  1:4648553/4‑100 (MQ=1)
                                                       cacaGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAtt                                         >  1:283096/4‑100 (MQ=11)
                                                        acaGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATACAGACAGCGACTCAGACGCAGATAGCGATTc                                        <  1:2402098/98‑1 (MQ=11)
                                                                 ctgATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcgga                               >  1:5810332/3‑94 (MQ=2)
                                                                 cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTTAGATTCAGATAGCGATTCAGATCCAGACAGCGACTCAGACGCAGATAGCGATTCAGAcgaaga                               >  1:804540/1‑95 (MQ=11)
                                                                 cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCCGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGa                               >  1:1479785/1‑100 (MQ=11)
                                                                 cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGa                               >  1:4890247/1‑100 (MQ=11)
                                                                 cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGa                               >  1:5853524/1‑100 (MQ=11)
                                                                 cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGa                               >  1:5594363/1‑100 (MQ=11)
                                                                  tgATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcggac                              >  1:4700714/2‑93 (MQ=2)
                                                                   gATGCGGACAGCGATTCAGACGCCGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGCCAGCGATTCAGACGCAGATAGCGATTCTGatgcggaca                             >  1:5194788/1‑92 (MQ=2)
                                                                   gATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGatgcggaca                             >  1:3850941/1‑92 (MQ=0)
                                                                   gATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGACTCTGatgcggaca                             >  1:37980/1‑92 (MQ=0)
                                                                   gATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACGGCGATTCAGACGCAGATAGCGATTCTGatgcggaca                             >  1:6292625/1‑92 (MQ=2)
                                                                   gATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcggaca                             >  1:2456012/1‑92 (MQ=2)
                                                                   gATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcggaca                             >  1:1327125/1‑92 (MQ=2)
                                                                   gATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcggaca                             >  1:621725/1‑92 (MQ=2)
                                                                   gATGCGGACAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGCTTCAGACGCAGTTAGCGATTCAGATTCAGATAGCGATTCTGatgcggaca                             >  1:5441170/1‑92 (MQ=0)
                                                                    ctgcGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAg                            >  1:3005421/4‑100 (MQ=2)
                                                                      tcagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCg                          >  1:3363472/4‑100 (MQ=1)
                                                                      gcGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCg                          >  1:1105978/2‑100 (MQ=11)
                                                                       cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                         >  1:5484034/3‑100 (MQ=11)
                                                                       cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                         >  1:1391311/3‑100 (MQ=11)
                                                                       cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                         >  1:2146989/3‑100 (MQ=11)
                                                                       cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                         >  1:3955793/3‑100 (MQ=11)
                                                                       cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                         >  1:3173560/3‑100 (MQ=11)
                                                                       cGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGa                         >  1:6370083/1‑100 (MQ=2)
                                                                        ggACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGAt                        >  1:2027849/1‑100 (MQ=2)
                                                                        agACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCTGACGCAGATAGCGATTCAGACGCAGATAGCGac                        >  1:4093735/2‑99 (MQ=11)
                                                                        agACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGac                        >  1:176495/2‑99 (MQ=11)
                                                                           cAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCa                     >  1:3905836/1‑100 (MQ=2)
                                                                                     gACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATa           >  1:5070029/1‑100 (MQ=35)
                                                                                     atggcAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATa           >  1:1555198/5‑100 (MQ=2)
                                                                                              aGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCgattcag  >  1:1423845/1‑100 (MQ=2)
                                                                                                   |                                                                                              
AGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAG  >  NC_007793/621766‑621959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑