Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 621,854 T→G S987A (TCA→GCA)  SAUSA300_RS02925 → MSCRAMM family adhesin SdrE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007793621,8540TG98.5% 740.6 / 9.0 817S987A (TCA→GCA) SAUSA300_RS02925MSCRAMM family adhesin SdrE
Reads supporting (aligned to +/- strand):  ref base T (12/0);  new base G (794/10);  total (807/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.45e-01

Only 100 of 819 total aligned reads displayed.

CAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAG  >  NC_007793/621759‑621951
                                                                                               |                                                                                                 
cagactcagacagcgactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGActcagactcaga                                                                                               >  1:3361543/1‑100 (MQ=25)
cagacgcagatagcgattcagatgcggacagTGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa                                                                                               >  1:4029003/1‑100 (MQ=2)
    ttatcccagcgattcagacgcagatagCGATTCTGATGCGCACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGc                                                                                           >  1:1004773/7‑100 (MQ=2)
       acagggcgactcagacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGat                                                                                        >  1:2740358/6‑99 (MQ=2)
                  cagatgcggacagcgattcagacgcagacagcgattcagacgcaGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGACtcagattcaga                                                                             >  1:3098806/9‑100 (MQ=2)
                  cagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcaga                                                                             >  1:1537446/1‑100 (MQ=255)
                  cagacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcaga                                                                             >  1:3810205/1‑100 (MQ=2)
                   tgacgcagatagCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcggac                                                                            >  1:3501881/2‑94 (MQ=0)
                   cgacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcagcc                                                                            >  1:1690216/2‑98 (MQ=2)
                   agacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcagac                                                                            >  1:4291587/1‑99 (MQ=2)
                    gacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcagaca                                                                           >  1:2495655/1‑98 (MQ=2)
                            aagcgattcagatgcgGACAGCGATTCAGAAGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtct                                                                   >  1:1411611/5‑99 (MQ=11)
                               ggattctgatgcgGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGAACAGCGATtcagac                                                                >  1:4941299/2‑100 (MQ=2)
                                  ctcagattccgaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGCTTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcg                                                             >  1:3630828/2‑96 (MQ=2)
                                  accacccgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgca                                                             >  1:1377372/9‑100 (MQ=2)
                                    ctgatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcaga                                                           >  1:2937919/9‑100 (MQ=2)
                                    ctgatgcggaCAGCGACGCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcaga                                                           >  1:566107/9‑100 (MQ=2)
                                    ccgacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                                           >  1:1325030/7‑100 (MQ=2)
                                    cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                                           >  1:1938930/1‑94 (MQ=11)
                                    cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                                           >  1:5935270/1‑94 (MQ=11)
                                    cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                                           >  1:2545974/1‑94 (MQ=11)
                                    cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                                           >  1:2626804/1‑94 (MQ=11)
                                    cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                                           >  1:1144004/1‑94 (MQ=11)
                                    cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                                           >  1:881015/1‑94 (MQ=11)
                                    cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATtcagacgcaga                                                           >  1:3725134/1‑100 (MQ=11)
                                    cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGACGCGGACAGCGATTAAGACGCTCATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                                           >  1:2287020/1‑94 (MQ=2)
                                    cagattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATtcagactcaga                                                           >  1:6125688/1‑100 (MQ=255)
                                    cagatgcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATtcagacgcaga                                                           >  1:3138081/1‑100 (MQ=2)
                                    cagacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                                           >  1:3781037/7‑100 (MQ=0)
                                    cagacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                                           >  1:3266920/7‑100 (MQ=0)
                                    cagacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                                           >  1:4341591/7‑100 (MQ=0)
                                     agacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGTGATTCTGATGCGGACAGCGATtcagacgcagat                                                          >  1:930333/6‑99 (MQ=0)
                                     agacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGAGTCTGATGCGGACAGCGATtcagacgcagat                                                          >  1:2019952/6‑99 (MQ=0)
                                      tacgcagaTAGCGACTCTGATGCGGACAGCGACTCAGATTCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACa                                                         >  1:1802516/5‑100 (MQ=11)
                                      gatgcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGCTGCGGACAGCGATtcagacgcagata                                                         >  1:5102881/7‑98 (MQ=2)
                                      gatgcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATtcagacgcagata                                                         >  1:1242416/7‑98 (MQ=2)
                                      gatgcggaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagata                                                         >  1:3060999/7‑98 (MQ=2)
                                      gatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcagata                                                         >  1:5308300/7‑98 (MQ=2)
                                      gatgcagaCAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagata                                                         >  1:6530459/1‑98 (MQ=2)
                                        ctcagaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGAAGCGGACAGc                                                       >  1:5146529/2‑100 (MQ=11)
                                         tcagaCAGCGATTCAGACACAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCg                                                      >  1:1899575/1‑100 (MQ=11)
                                         gcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCg                                                      >  1:6459498/4‑100 (MQ=2)
                                         gcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCg                                                      >  1:1270589/4‑100 (MQ=2)
                                         ccggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGCTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCg                                                      >  1:6192255/4‑100 (MQ=2)
                                          cggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                     >  1:2079537/3‑100 (MQ=2)
                                          cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGCCAGCGa                                                     >  1:1636687/1‑100 (MQ=2)
                                          cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCgt                                                     >  1:6301957/1‑99 (MQ=11)
                                          cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                     >  1:27948/1‑100 (MQ=11)
                                          cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                     >  1:5395213/1‑100 (MQ=11)
                                          cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                     >  1:1194979/1‑100 (MQ=11)
                                          cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                     >  1:1460514/1‑100 (MQ=11)
                                          cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                     >  1:1995082/1‑100 (MQ=11)
                                          cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                     >  1:3547800/1‑100 (MQ=11)
                                          cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                     >  1:601204/1‑100 (MQ=11)
                                          cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                     >  1:6072099/1‑100 (MQ=11)
                                          cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                     >  1:6380405/1‑100 (MQ=11)
                                          cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                     >  1:6227559/1‑100 (MQ=11)
                                           agaTAGCGATTCAGATTCAGATAGCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCAGATTCTGATAACGACTCTGATGCGGACAGCGAc                                                    >  1:5031291/1‑100 (MQ=2)
                                            tgcAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGTCAGCGatt                                                   >  1:2876723/3‑98 (MQ=2)
                                              cAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTTAGATAGCGATTCTGATGCGGACAGCGATtca                                                 >  1:4530179/1‑100 (MQ=2)
                                              cAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGATTCAGACGCAGATAGCGATtca                                                 >  1:5811473/1‑100 (MQ=2)
                                               aGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGCTtcag                                                >  1:4462548/1‑100 (MQ=2)
                                                      ccgacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                         >  1:2193026/7‑100 (MQ=2)
                                                      cagattcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCACACAGCGATtcagactcaga                                         >  1:3888702/1‑100 (MQ=2)
                                                      cagacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                         >  1:723468/7‑100 (MQ=2)
                                                        aacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGACTCTGATGCGGACAGCGACTCAGACGCAGATa                                       >  1:3396895/5‑100 (MQ=2)
                                                           gcagatAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCg                                    >  1:5857551/2‑100 (MQ=11)
                                                           ccagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCg                                    >  1:6588156/2‑100 (MQ=11)
                                                            cagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCGGATTCAGATAGCGa                                   >  1:4848088/1‑100 (MQ=2)
                                                            cagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGa                                   >  1:685401/1‑100 (MQ=11)
                                                             tgatAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGAt                                  >  1:804915/2‑100 (MQ=11)
                                                             tgacaGCGCCTCAGTTTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAt                                  >  1:5247547/5‑100 (MQ=1)
                                                             agatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGACTCAGACGCAGATAGCGAt                                  >  1:4385546/1‑100 (MQ=11)
                                                             agatAGCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGACTCAGACGCAGATAGCGACTATGATACGGACAGCGACTCAGATTCAGACAGCGAt                                  >  1:2687747/1‑100 (MQ=2)
                                                              gacaGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAtt                                 >  1:4648553/4‑100 (MQ=1)
                                                                  gCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGa                             >  1:2455196/1‑100 (MQ=2)
                                                                     attctgATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTc                          <  1:3176596/95‑1 (MQ=2)
                                                                     aCTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCTGACGCAGATAGCGATTCAGAcgc                          >  1:489406/1‑98 (MQ=11)
                                                                        ttgATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGatgcgga                       >  1:551923/3‑94 (MQ=0)
                                                                        ctgATGCGGACAGCGACTCAGACGCAGATAGCGACTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGAcgctga                       >  1:3847925/3‑95 (MQ=2)
                                                                        cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGa                       >  1:5594363/1‑100 (MQ=11)
                                                                         tgATGCGGACAGCGATTCAGACGCAGATAGCGATTCTAATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGACTCTGatgcggac                      >  1:4224296/2‑93 (MQ=0)
                                                                         tgATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcggac                      >  1:4700714/2‑93 (MQ=2)
                                                                         gccaccagACAGCGATTCAGACGCAGATAGCGATTCAGCTTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGat                      >  1:4200339/8‑99 (MQ=11)
                                                                          gATGCGGACAGCGATTCAGACGCCGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGCCAGCGATTCAGACGCAGATAGCGATTCTGatgcggaca                     >  1:5194788/1‑92 (MQ=2)
                                                                          gATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGACTCTGatgcggaca                     >  1:6403191/1‑92 (MQ=0)
                                                                          gATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGACTCTGatgcggaca                     >  1:5721654/1‑92 (MQ=0)
                                                                          gATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcggaca                     >  1:4129588/1‑92 (MQ=2)
                                                                          gATGCGGACAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGCTTCAGACGCAGTTAGCGATTCAGATTCAGATAGCGATTCTGatgcggaca                     >  1:5441170/1‑92 (MQ=0)
                                                                           ctgcGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAg                    >  1:3005421/4‑100 (MQ=2)
                                                                           atgcGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAg                    >  1:1075677/4‑100 (MQ=2)
                                                                            gtcagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGCTAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGc                   >  1:4804179/5‑100 (MQ=1)
                                                                              gggACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGATAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGAATCTGATGCGGACAGCGa                 >  1:3444765/2‑100 (MQ=2)
                                                                              cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                 >  1:2391650/3‑100 (MQ=11)
                                                                              cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                 >  1:2130163/3‑100 (MQ=11)
                                                                              cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                 >  1:3955793/3‑100 (MQ=11)
                                                                              cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                 >  1:1104702/3‑100 (MQ=11)
                                                                              cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                 >  1:5484034/3‑100 (MQ=11)
                                                                               agACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCTGACGCAGATAGCGATTCAGACGCAGATAGCGac                >  1:4093735/2‑99 (MQ=11)
                                                                               agACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGac                >  1:176495/2‑99 (MQ=11)
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CAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAG  >  NC_007793/621759‑621951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑