Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 621,836 T→G S981A (TCG→GCG)  SAUSA300_RS02925 → MSCRAMM family adhesin SdrE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007793621,8360TG92.3% 473.8 / 94.2 714S981A (TCG→GCG) SAUSA300_RS02925MSCRAMM family adhesin SdrE
Reads supporting (aligned to +/- strand):  ref base T (53/2);  new base G (650/8);  total (704/10)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

Only 100 of 716 total aligned reads displayed.

TCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACA  >  NC_007793/621746‑621932
                                                                                          |                                                                                                
gcagacagcgactcagacgcagatagcgattcagatgCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGACTCCGATGCGGACcgcg                                                                                         >  1:1958130/2‑100 (MQ=11)
                 ttatcccagcgattcagacgcagatagCGATTCTGATGCGCACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGc                                                                        >  1:1004773/7‑100 (MQ=2)
                 ctcagacagcgactcagacgcagatagCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGAAGCAGATAGc                                                                        >  1:6634854/1‑100 (MQ=2)
                      atagcgactcagacgcagatcgCGACTCTGATGCGGACAGCGACTCAGACGCAGATCGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtc                                                                   >  1:5824753/3‑100 (MQ=2)
                        cgcgactcagacgcagatagCGATTCAGATGCGGACAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtcag                                                                 >  1:3809096/2‑100 (MQ=11)
                          tgtggcagacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgat                                                               >  1:3644317/6‑100 (MQ=2)
                          tgactcagacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgat                                                               >  1:1993531/2‑100 (MQ=2)
                               cagatgcggacagcgattcagacgcagacagcgattcagacgcaGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGACtcagattcaga                                                          >  1:3098806/9‑100 (MQ=2)
                               cagacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcaga                                                          >  1:2595148/1‑100 (MQ=2)
                               cagacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcaga                                                          >  1:2079915/1‑100 (MQ=2)
                                agacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcagac                                                         >  1:4291587/1‑99 (MQ=2)
                                 gacgcagatagCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcggaca                                                        >  1:4040136/1‑93 (MQ=0)
                                 gacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcagaca                                                        >  1:6537472/1‑98 (MQ=2)
                                         tagcgattcagatgcgGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGACtca                                                >  1:5074594/5‑100 (MQ=1)
                                         aagcgactcagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGGTTCAGATAGCGATtct                                                >  1:5554264/8‑99 (MQ=1)
                                          agcgattcagatgcgGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctg                                               >  1:3839523/4‑98 (MQ=1)
                                          agcgattcagatgcgGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctg                                               >  1:6412924/4‑98 (MQ=1)
                                           cagattcagatgcaGACAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATtctga                                              >  1:2298134/3‑100 (MQ=11)
                                             tattcagattcagACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGCTAGCGATtctgatg                                            >  1:4858439/2‑98 (MQ=11)
                                             gattcagatgcgGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatg                                            >  1:6236350/1‑98 (MQ=11)
                                               ctcagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcg                                          >  1:5176113/2‑96 (MQ=11)
                                                ccagattcagaCAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcag                                         >  1:896986/2‑100 (MQ=11)
                                                 ctgatgcggaCAGCGATTCAGACGCGGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                        >  1:1772038/9‑100 (MQ=2)
                                                 ctgatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcaga                                        >  1:2250189/9‑100 (MQ=2)
                                                 ctgatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcaga                                        >  1:246719/9‑100 (MQ=2)
                                                 cagattcagtCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATACAGACGCAGATAGCGATTCCGATTCAGATAGCGATtctgttgcgga                                        >  1:3522333/1‑91 (MQ=2)
                                                 cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                        >  1:1174340/1‑94 (MQ=11)
                                                 cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                        >  1:2684762/1‑94 (MQ=11)
                                                 cagattcagaCAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                        >  1:3398899/1‑100 (MQ=11)
                                                 cagacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                        >  1:5960208/7‑100 (MQ=0)
                                                 cagacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                        >  1:822816/7‑100 (MQ=0)
                                                 cagacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                        >  1:4341591/7‑100 (MQ=0)
                                                  agattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcggac                                       >  1:733087/1‑93 (MQ=11)
                                                  agacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGTGATTCTGATGCGGACAGCGATtcagacgcagat                                       >  1:930333/6‑99 (MQ=0)
                                                  agacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagat                                       >  1:3371473/6‑99 (MQ=0)
                                                  agacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagat                                       >  1:3454406/6‑99 (MQ=0)
                                                   gattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcggaca                                      >  1:5884139/1‑92 (MQ=11)
                                                   gatgcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATtcagacgcagata                                      >  1:294536/7‑98 (MQ=2)
                                                   gatgcggaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagata                                      >  1:5143488/7‑98 (MQ=2)
                                                   cacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACtcagacgcagatc                                      >  1:6135782/5‑98 (MQ=1)
                                                    cggcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAg                                     >  1:1416421/6‑100 (MQ=2)
                                                     tgcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGc                                    >  1:3573460/5‑100 (MQ=2)
                                                      tcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCg                                   >  1:1533908/1‑100 (MQ=11)
                                                      tcagaCAGCGATTCAGACACAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCg                                   >  1:1899575/1‑100 (MQ=11)
                                                      gcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCCGATAGCGATTCTGATGCGGACAGCg                                   >  1:3794447/4‑100 (MQ=2)
                                                      gcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCg                                   >  1:1270589/4‑100 (MQ=2)
                                                       cagaCAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGCTAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGa                                  >  1:3972225/1‑100 (MQ=2)
                                                       cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCCGATAGCGATTCAGATTCAGCTAGCGATTCTGATGCGGACAGCGa                                  >  1:1704504/1‑100 (MQ=2)
                                                       cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAgatc                                  >  1:2027906/1‑97 (MQ=2)
                                                       cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCgt                                  >  1:6301957/1‑99 (MQ=11)
                                                       cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                  >  1:3273676/1‑100 (MQ=11)
                                                       cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                  >  1:4903580/1‑100 (MQ=11)
                                                       cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                  >  1:4808293/1‑100 (MQ=11)
                                                       cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                  >  1:4966323/1‑100 (MQ=11)
                                                       cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                  >  1:27948/1‑100 (MQ=11)
                                                       cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                  >  1:546093/1‑100 (MQ=11)
                                                       cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                  >  1:2430916/1‑100 (MQ=11)
                                                       cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                  >  1:5639408/1‑100 (MQ=11)
                                                       cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                  >  1:6027450/1‑100 (MQ=11)
                                                       cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                  >  1:6072099/1‑100 (MQ=11)
                                                       cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                  >  1:1379063/1‑100 (MQ=11)
                                                       cagaCAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGATTCAGACGCAGATAGCGa                                  >  1:3066348/1‑100 (MQ=11)
                                                        atgcAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGat                                 >  1:1250653/4‑99 (MQ=2)
                                                        agaTAGCGATTCAGATTCAGATAGCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCAGATTCTGATAACGACTCTGATGCGGACAGCGAc                                 >  1:5031291/1‑100 (MQ=2)
                                                         tgcAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGTCAGCGatt                                >  1:2876723/3‑98 (MQ=2)
                                                         caCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGatt                                >  1:5376805/2‑98 (MQ=2)
                                                          ccAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGAAtc                               >  1:5514970/2‑100 (MQ=2)
                                                          ccAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagatgtcgattc                               >  1:2760218/2‑91 (MQ=2)
                                                          ataGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGGGATtc                               <  1:3143623/98‑1 (MQ=2)
                                                           cAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTTAGATAGCGATTCTGATGCGGACAGCGATtca                              >  1:4530179/1‑100 (MQ=2)
                                                            aGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGCTtcag                             >  1:4462548/1‑100 (MQ=2)
                                                                   cgctggcagatAGCGACTCTGATGCGGACAGCGCCTCAGACGCAGATAGCGACTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACtcagattcaga                      >  1:3729352/7‑100 (MQ=2)
                                                                   cagacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATCCAGATTCAGATAGCGATTATGATGCGGACAGCGATtcagacgcaga                      >  1:568846/7‑100 (MQ=2)
                                                                    gaccccagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagat                     >  1:121921/6‑100 (MQ=0)
                                                                    cgacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCCGATTCAGATAGCGATTCTGATGCGGGCAGCGATtcagacgcagat                     >  1:6922/6‑100 (MQ=2)
                                                                    agattcagatAGCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGACTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACtcagatgcagac                     <  1:4223672/100‑2 (MQ=11)
                                                                       cgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGc                  >  1:6194397/3‑100 (MQ=2)
                                                                        tgtgatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCg                 >  1:4662597/4‑100 (MQ=2)
                                                                        tcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCg                 >  1:1120930/1‑100 (MQ=11)
                                                                         cagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGa                >  1:301003/1‑100 (MQ=11)
                                                                          ccatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGGTAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGAt               >  1:5214882/3‑100 (MQ=2)
                                                                          agatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGACTCAGACGCAGATAGCGAt               >  1:4385546/1‑100 (MQ=11)
                                                                          agatAGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGAt               >  1:574837/1‑100 (MQ=2)
                                                                              aGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTctg           >  1:5421324/1‑98 (MQ=2)
                                                                              aGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTcag           <  1:4182668/100‑3 (MQ=11)
                                                                                  gatctgATGCGGACAGCGACTCAGACGCAGATAGCGACTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGGCAGCGATTCGGAcgc       <  1:5301699/95‑3 (MQ=0)
                                                                                  catgggATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGACTCAGACAGCGATTCTGatgc       >  1:2529571/6‑97 (MQ=2)
                                                                                    tCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGAGTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGatgcgg     >  1:2152904/1‑95 (MQ=2)
                                                                                    cctgATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcgg     >  1:181684/4‑95 (MQ=2)
                                                                                     ctgATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcgga    >  1:6480265/3‑94 (MQ=2)
                                                                                     cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCGGACGCAGa    >  1:318936/1‑100 (MQ=11)
                                                                                     cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGa    >  1:1467790/1‑100 (MQ=11)
                                                                                     cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGa    >  1:3881615/1‑100 (MQ=11)
                                                                                     cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGa    >  1:1087507/1‑100 (MQ=11)
                                                                                      tgATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcggac   >  1:4700714/2‑93 (MQ=2)
                                                                                       gATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGata  >  1:4098062/1‑98 (MQ=11)
                                                                                       gATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGatgcggaca  >  1:6382565/1‑92 (MQ=0)
                                                                                       gATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcggaca  >  1:1628174/1‑92 (MQ=2)
                                                                                       gATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcggaca  >  1:1327125/1‑92 (MQ=2)
                                                                                       gATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcggaca  >  1:4129588/1‑92 (MQ=2)
                                                                                          |                                                                                                
TCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACA  >  NC_007793/621746‑621932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑