Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 621,829 C→T D978D (GAC→GAT)  SAUSA300_RS02925 → MSCRAMM family adhesin SdrE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007793621,8290CT93.4% 459.0 / 38.4 604D978D (GAC→GAT) SAUSA300_RS02925MSCRAMM family adhesin SdrE
Reads supporting (aligned to +/- strand):  ref base C (36/4);  new base T (559/4);  total (596/8)
Fisher's exact test for biased strand distribution p-value = 9.63e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 609 total aligned reads displayed.

CAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCA  >  NC_007793/621735‑621928
                                                                                              |                                                                                                   
                        cagactcagacagcgactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGActcagactcaga                                                                        >  1:3361543/1‑100 (MQ=25)
                        cagacgcagatagcgtctcagacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGa                                                                        >  1:3700804/1‑100 (MQ=2)
                            ttatcccagcgattcagacgcagatagCGATTCTGATGCGCACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGc                                                                    >  1:1004773/7‑100 (MQ=2)
                                 atagcgactcagacgcagatcgCGACTCTGATGCGGACAGCGACTCAGACGCAGATCGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtc                                                               >  1:5824753/3‑100 (MQ=2)
                                       cctcagacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGCTAGCGATtctgatgc                                                         >  1:3801882/2‑98 (MQ=2)
                                          cggacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcaga                                                      >  1:4061804/3‑100 (MQ=2)
                                          cagatgcggacagcgattcagacgcagacagcgattcagacgcaGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGACtcagattcaga                                                      >  1:3098806/9‑100 (MQ=2)
                                          cagacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcaga                                                      >  1:1088490/1‑100 (MQ=2)
                                          cagacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcaga                                                      >  1:6085354/1‑100 (MQ=2)
                                          cagacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcaga                                                      >  1:3546540/1‑100 (MQ=2)
                                          cagacgcagatagCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcaga                                                      >  1:1981632/1‑100 (MQ=2)
                                          cagacacagatagCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATtctgatgcgga                                                      >  1:1247541/1‑95 (MQ=0)
                                             acccggacagCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCCGTTAGCGACTCAGCTTCAGATAg                                                   >  1:2978039/6‑100 (MQ=2)
                                              accagatccagattcagatgcgGACAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGc                                                  >  1:3924690/11‑100 (MQ=2)
                                               gcagatagCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGATGCAGATAGCg                                                 >  1:3163205/2‑100 (MQ=11)
                                                   gcagCGATTGAGACGCAGACAGCGATTCAGAGGCAGATAGCGATACTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGaccc                                             <  1:6144271/99‑4 (MQ=2)
                                                     agcgattcagatgcgGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGCTTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctg                                           >  1:5017585/4‑98 (MQ=0)
                                                      cagattcagatgcaGACAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATtctga                                          >  1:2298134/3‑100 (MQ=11)
                                                        gattcagatgcgGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatg                                        >  1:6236350/1‑98 (MQ=11)
                                                          gccagacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgca                                      >  1:5399113/9‑100 (MQ=0)
                                                          cccagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGCTtctgacgcg                                      >  1:6518824/3‑97 (MQ=11)
                                                           ccagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgg                                     >  1:3852429/2‑95 (MQ=11)
                                                            ctgatgcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATtcagacgcaga                                    >  1:6345301/9‑100 (MQ=2)
                                                            ctgatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcaga                                    >  1:2250189/9‑100 (MQ=2)
                                                            ctgatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcaga                                    >  1:246719/9‑100 (MQ=2)
                                                            ccgatgcggaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGATtctgacgcaga                                    >  1:552400/9‑100 (MQ=0)
                                                            cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                    >  1:3394458/1‑94 (MQ=11)
                                                            cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                    >  1:4115903/1‑94 (MQ=11)
                                                            cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                    >  1:3813640/1‑94 (MQ=11)
                                                            cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                    >  1:1174340/1‑94 (MQ=11)
                                                            cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                    >  1:2120235/1‑94 (MQ=11)
                                                            cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                    >  1:1938930/1‑94 (MQ=11)
                                                            cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgga                                    >  1:3619956/1‑94 (MQ=11)
                                                            cagattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcgaa                                    >  1:6394095/1‑94 (MQ=11)
                                                            cagattcagaCAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                    >  1:2670252/1‑100 (MQ=11)
                                                            cagatgcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATtcagacgcaga                                    >  1:3138081/1‑100 (MQ=2)
                                                            cagacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGTTtcagacgcaga                                    >  1:2446726/7‑100 (MQ=0)
                                                            cagacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                    >  1:4341591/7‑100 (MQ=0)
                                                             tgatgcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATtcagacgcagat                                   >  1:400975/8‑99 (MQ=2)
                                                             ctctgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcagat                                   >  1:847294/8‑99 (MQ=2)
                                                             acatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcagag                                   >  1:145265/8‑99 (MQ=2)
                                                              tacgcagaTAGCGACTCTGATGCGGACAGCGACTCAGATTCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACa                                  >  1:1802516/5‑100 (MQ=11)
                                                              gattcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATtctgatgcggaca                                  >  1:5884139/1‑92 (MQ=11)
                                                              gatgcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGAAAGCGATTCAGCTGCGGACAGCGATtcagacgcagata                                  >  1:4216073/7‑98 (MQ=0)
                                                              gatgcggaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagata                                  >  1:3060999/7‑98 (MQ=2)
                                                              gatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcagata                                  >  1:5308300/7‑98 (MQ=2)
                                                               gtgcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATCCAGACAGCGTTTCTGATGCGGACAg                                 >  1:4841777/6‑100 (MQ=2)
                                                               ctgcagaTAGCGATTCAGATTCAGATAGCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGACTCAGACGCAGATAGCGACTCTGATGCAGACAg                                 >  1:5738999/4‑100 (MQ=2)
                                                                ttcagaCAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagatcgg                                >  1:2835328/1‑96 (MQ=11)
                                                                tgcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGc                                >  1:5495706/5‑100 (MQ=2)
                                                                tgcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGc                                >  1:5632659/5‑100 (MQ=2)
                                                                ctcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGc                                >  1:348546/2‑100 (MQ=11)
                                                                ctcagaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGAAGCGGACAGc                                >  1:5146529/2‑100 (MQ=11)
                                                                 tcagaCAGCGATTCAGACGCAGGTAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCCGATTCGGATAGCGAATCTGACGCGGACAGCg                               >  1:1729530/1‑100 (MQ=2)
                                                                 tcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCg                               >  1:5669076/1‑100 (MQ=11)
                                                                 tcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCAGACAGCGATTCTGACGCGGACAGCg                               >  1:6580672/1‑100 (MQ=2)
                                                                 tcagaCAGCGATTCAGACACAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCg                               >  1:1899575/1‑100 (MQ=11)
                                                                 gcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCg                               >  1:3430254/4‑100 (MQ=2)
                                                                 gcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGAGAGCGATTCTGACGCGGACAGCg                               >  1:1317798/4‑100 (MQ=2)
                                                                 gcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAgat                               >  1:2716710/2‑98 (MQ=2)
                                                                  cggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                              >  1:2079537/3‑100 (MQ=2)
                                                                  cagaCAGCGATTCAGACGCAGATAGCGATTCTTATGCGGACAGCGATTCAGGCGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                              >  1:5963749/1‑100 (MQ=2)
                                                                  cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGTGAGTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                              >  1:1672126/1‑100 (MQ=2)
                                                                  cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                              >  1:949588/1‑100 (MQ=11)
                                                                  cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                              >  1:5108427/1‑100 (MQ=11)
                                                                  cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                              >  1:5218477/1‑100 (MQ=11)
                                                                  cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                              >  1:4777095/1‑100 (MQ=11)
                                                                  cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                              >  1:1611886/1‑100 (MQ=11)
                                                                  cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGACGCGGACAGCGa                              >  1:2526972/1‑100 (MQ=11)
                                                                  cagaCAGCGATTAAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                              >  1:1132983/1‑100 (MQ=2)
                                                                  cagaCAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagatcggaa                              >  1:1514788/1‑94 (MQ=2)
                                                                  cagaCAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGACAGCGa                              >  1:794435/1‑100 (MQ=11)
                                                                   tgataGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGat                             >  1:276907/5‑99 (MQ=0)
                                                                   agaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGat                             >  1:2019705/1‑99 (MQ=11)
                                                                   acccAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAAACGCAGATAgtaat                             >  1:4927245/4‑96 (MQ=2)
                                                                    gaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGatt                            >  1:2593336/1‑98 (MQ=2)
                                                                     ccAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATtc                           >  1:1398969/2‑100 (MQ=11)
                                                                      gtgCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGACtca                          >  1:666779/3‑100 (MQ=2)
                                                                      cAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTTAGATAGCGATTCTGATGCGGACAGCGATtca                          >  1:4530179/1‑100 (MQ=2)
                                                                       aGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGCTtcag                         >  1:4462548/1‑100 (MQ=2)
                                                                              ccgacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                  >  1:5184346/7‑100 (MQ=2)
                                                                              cagacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATTGCGATTCTGATGCGGACAGCGATtcagacgcaga                  >  1:2917995/7‑100 (MQ=0)
                                                                              cagacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                  >  1:5447487/7‑100 (MQ=2)
                                                                              cagacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                  >  1:6434524/7‑100 (MQ=2)
                                                                               gaccccagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagat                 >  1:121921/6‑100 (MQ=0)
                                                                                  cgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGc              >  1:6194397/3‑100 (MQ=2)
                                                                                   tcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCg             >  1:6039936/1‑100 (MQ=2)
                                                                                   tcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGCTTCTGACAGCg             >  1:3758130/1‑100 (MQ=2)
                                                                                   gcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCg             >  1:3273445/2‑100 (MQ=11)
                                                                                   gcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCg             >  1:4293703/2‑100 (MQ=11)
                                                                                   gcagatAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCg             >  1:912709/2‑100 (MQ=11)
                                                                                    cagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGa            >  1:1266195/1‑100 (MQ=2)
                                                                                    cagatAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGa            >  1:5053104/1‑100 (MQ=11)
                                                                                     agatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGAt           >  1:4697917/1‑100 (MQ=11)
                                                                                     agatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGACTCAGACGCAGATAGCGAt           >  1:4385546/1‑100 (MQ=11)
                                                                                     agatAGCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGACTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGAt           >  1:4175865/1‑100 (MQ=11)
                                                                                      gatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGCTTCAGATAGCGact          >  1:717914/1‑98 (MQ=2)
                                                                                      gacaGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGAtt          >  1:4648553/4‑100 (MQ=1)
                                                                                             aCTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCTGACGCAGATAGCGATTCAGAcgc   >  1:489406/1‑98 (MQ=11)
                                                                                              cTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATTCAGATAGCGAtactgatgcg  >  1:1353123/1‑91 (MQ=2)
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CAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCA  >  NC_007793/621735‑621928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑