| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 67,926 | T→C | intergenic (‑944/‑40) | SAUSA300_RS00300 ← / → SAUSA300_RS00305 | hypothetical protein/transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 67,926 | 0 | T | C | 92.3% | 25.0 / ‑3.5 | 13 | intergenic (‑944/‑40) | SAUSA300_RS00300/SAUSA300_RS00305 | hypothetical protein/transposase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (7/5); minor base A (1/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
TACGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTTAAAAAAGTACAAGGTATTGGAAAGGAAGTGGTACCAACAGTAGTCAT > NC_007793/67829‑67958 | caCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCAtt > 1:406938/2‑100 (MQ=255) aCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAGAGTACAAGGCAtta > 1:4907242/1‑99 (MQ=255) aCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTg > 1:110105/1‑100 (MQ=255) aCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTg > 1:2403616/1‑100 (MQ=255) aCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTg > 1:5338146/1‑100 (MQ=255) aCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGAATTg > 1:1384590/1‑100 (MQ=255) taggCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTGGATAGGAAGTGGTAcc > 1:3511493/4‑100 (MQ=255) gCTGTCAGAAGGAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTAGATCGGAAgagcacacgtc > 1:554949/1‑90 (MQ=255) gAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTGGAAAGGAAGTGGTACCAACAGTAGTc < 1:1104708/100‑1 (MQ=255) gAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTGGAAAGGAAGTGGTACCAACAGTAGTc < 1:6396514/100‑1 (MQ=255) aGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTGGAAAGGAAGTGGTACCAACAGTAGTCAt < 1:3688598/100‑1 (MQ=255) aGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTGGAAAGGAAGTGGTACCAACAGTAGTCAt < 1:4194097/100‑1 (MQ=255) aGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTGGAAAGGAAGTGGTACCAACAGTAGTCAt < 1:4646498/100‑1 (MQ=255) | TACGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTTAAAAAAGTACAAGGTATTGGAAAGGAAGTGGTACCAACAGTAGTCAT > NC_007793/67829‑67958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |