Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	617,707	A→C	G1117G (GGA→GGC)	SAUSA300_RS02920 →	serine‑aspartate repeat‑containing protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	617,707	0	A	C	100.0%	48.2 / NA	24	G1117G (GGA→GGC)	SAUSA300_RS02920	serine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/3);  total (21/3)

CAGGTACAAATACAACTGAAGATGATAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGATACTACGAAGAAGAAACATCAGATAGCGACT‑CAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACT  >  NC_007793/617613‑617798                                                                                               |                                                                                             cAGGTACAAATACAACTGAAGATGATAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGctacta                                                                                         >  1:1822447/1‑100 (MQ=17)      aCAAATACAACTGAAGATGATAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACgaag                                                                                    >  1:2727923/1‑100 (MQ=17)            aCAACTGAAGATGATAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGaaa                                                                              >  1:4715439/1‑100 (MQ=17)                                 gCAGATGGTGGCGAAGTTGACGTAATAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATTAGATAGCGaaccgagat                                                         >  1:1494111/1‑92 (MQ=11)                                 gCAGATGGTGGCGAAGTTGACGTAACACTTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCTGCC‑CAGAtc                                                        >  1:6254680/1‑99 (MQ=11)                                 gCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAGTGGCTACTACGAAGAAGAAACATCAGATAGCGACT‑CAGAtc                                                        >  1:4751548/1‑99 (MQ=12)                                 gCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTACAGAt                                                         >  1:4016110/1‑100 (MQ=255)                                 gCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACT‑cgagat                                                        >  1:3105776/1‑95 (MQ=255)                                 gCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCAACC‑CAGAtc                                                        >  1:2906977/1‑99 (MQ=255)                                 gCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGC‑ACT‑CAGAtcg                                                       >  1:5164397/1‑98 (MQ=255)                                 gCAGATGGTGGCGAAGTTGACGTAACAATTACGGATAATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGTAACT‑CAGAtc                                                        >  1:1022652/1‑99 (MQ=11)                                  cAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGAGACT‑CAGAtcg                                                       >  1:4420876/1‑98 (MQ=9)                                   aGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACT‑CAGATTCg                                                      >  1:1415288/1‑100 (MQ=14)                                      tggtggCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACT‑CAGATTCGGAc                                                   >  1:1382264/1‑100 (MQ=17)                                        gtggCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACT‑CAGATTCGGACAg                                                 >  1:4157048/1‑100 (MQ=17)                                              caGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAAGATCCGATTGCGACT‑CAGATTTGGACAGCGACTc                                           >  1:4426852/2‑100 (MQ=11)                                              aaGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATGATGGCTACTACGAAGAAGAAACATCAGATAGCGACT‑CAGATTCGGACAGCGACTc                                           <  1:3320234/100‑1 (MQ=255)                                                                          tgatTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACT‑CAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGAt               <  1:4078325/100‑1 (MQ=255)                                                                             tttCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGTTAGCGACT‑CAGATTCGGACAGCGACTCAGATTCCGACAGAGACTCAGACTCAGATAGt            >  1:4665884/1‑100 (MQ=255)                                                                                cacaCTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACT‑CAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGAt         >  1:5972672/1‑100 (MQ=255)                                                                                cacaCTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACT‑CAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAAACTCAGATAGCGAt         >  1:4824185/1‑100 (MQ=255)                                                                                   acTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACT‑CAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTcg      <  1:313541/100‑2 (MQ=255)                                                                                      tGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACT‑CAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGat   >  1:1557380/1‑99 (MQ=255)                                                                                      tGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACT‑CAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGAGGTTCAGat   >  1:2553345/1‑99 (MQ=255)                                                                                       gATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACT‑CAGATTCAGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTAAGatc  >  1:3308832/1‑98 (MQ=17)                                                                                               |                                                                                             CAGGTACAAATACAACTGAAGATGATAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGATACTACGAAGAAGAAACATCAGATAGCGACT‑CAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACT  >  NC_007793/617613‑617798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑