Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	62,578	G→A	A4V (GCT→GTT)	SAUSA300_RS00270 ←	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	62,578	0	G	A	100.0%	10.6 / NA	6	A4V (GCT→GTT)	SAUSA300_RS00270	transposase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/3);  total (3/3)

CTAATATTTAATTCTTTCATAATTTCTTTTGTTGGAAATCCTGCTGCTTTCATTTCAACTGCTTTATACTTTGTTTCTACTGAATAAGAAGCTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAGTGTTTTTATATAATTCCCACT‑ATTGGGGTC  >  NC_007793/62488‑62667                                                                                           |                                                                                           cTAATATTTAATTCTTTCATAATTTCTTTTGTTGAAAATCCTGCTACTTTCATTTCAACTGCTTTATACTTTGTTTCTACTGAATAAGAAACTCTTTTCa                                                                                   >  1:2787583/1‑100 (MQ=255)                     aaTTTCTTTTGTTGAAAATCCTGCTACTTTCATTTCGACTGCTTTATACTTTGTTTCTACTGAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGtc                                                               <  1:722948/100‑2 (MQ=255)                                              actTTCATTTCAACTGCTTTATACTTTGTTTCTACTGAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAAc                                      >  1:4536374/2‑100 (MQ=255)                                                        caACTGCTTTATACTTTGTTTCTACTGAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACAAAAGTGttt                            >  1:255158/1‑100 (MQ=255)                                                                                  gAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACAAAAGTGTTTTTATATAATTCCCACTAATTGGGGTc  <  1:3806674/100‑1 (MQ=255)                                                                                  gAATAAGAAACCCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACAAAAGTGTTTTTATATAATTCCCACTAATTGGGGTc  <  1:2275236/100‑1 (MQ=255)                                                                                           |                                                                                           CTAATATTTAATTCTTTCATAATTTCTTTTGTTGGAAATCCTGCTGCTTTCATTTCAACTGCTTTATACTTTGTTTCTACTGAATAAGAAGCTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAGTGTTTTTATATAATTCCCACT‑ATTGGGGTC  >  NC_007793/62488‑62667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑