Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	62,533	G→A	A19V (GCA→GTA)	SAUSA300_RS00270 ←	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	62,533	0	G	A	100.0%	10.6 / NA	6	A19V (GCA→GTA)	SAUSA300_RS00270	transposase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/5);  total (1/5)

CTTTCCCCATTTCGATACCATCGCCACCAATTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTGTTGGAAATCCTGCTGCTTTCATTTCAACTGCTTTATACTTTGTTTCTACTGAATAAGAAGCTCTTTTCATAGAAAAAAACACCTCCGTA  >  NC_007793/62437‑62607                                                                                                 |                                                                           cTTTCCCTATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTGTTGAAAATCCTGCTACtt                                                                         <  1:368995/100‑1 (MQ=255) cTTTCCCTATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTGTTGAAAATCCTGCTACtt                                                                         <  1:4056677/100‑1 (MQ=255)                tACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTGTTGAAAATCCTGCTACTTTCATTTCAACTGCta                                                          <  1:5603318/100‑2 (MQ=255)                       gCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTGTTGAAAATCCTGCTACTTTCATTTCAACTGCTTTATACtt                                                   <  1:2358190/100‑1 (MQ=255)                                                    cTAATATTTAATTCTTTCATAATTTCTTTTGTTGAAAATCCTGCTACTTTCATTTCAACTGCTTTATACTTTGTTTCTACTGAATAAGAAACTCTTTTCa                      >  1:2787583/1‑100 (MQ=255)                                                                        aaTTTCTTTTGTTGAAAATCCTGCTACTTTCATTTCGACTGCTTTATACTTTGTTTCTACTGAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGtc  <  1:722948/100‑2 (MQ=255)                                                                                                 |                                                                           CTTTCCCCATTTCGATACCATCGCCACCAATTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTGTTGGAAATCCTGCTGCTTTCATTTCAACTGCTTTATACTTTGTTTCTACTGAATAAGAAGCTCTTTTCATAGAAAAAAACACCTCCGTA  >  NC_007793/62437‑62607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑